##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547548_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4439903
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40060762588732	31.0	31.0	33.0	30.0	34.0
2	31.808300316470877	31.0	31.0	34.0	30.0	34.0
3	31.805533138899655	31.0	31.0	34.0	30.0	34.0
4	35.62815133573864	37.0	35.0	37.0	33.0	37.0
5	35.561541772421606	37.0	35.0	37.0	33.0	37.0
6	35.61571682984966	37.0	35.0	37.0	33.0	37.0
7	36.01566926124287	37.0	35.0	37.0	35.0	37.0
8	35.98076061571616	37.0	35.0	37.0	35.0	37.0
9	37.63645421983318	39.0	38.0	39.0	35.0	39.0
10	37.18166095070095	39.0	37.0	39.0	33.0	39.0
11	36.936400412351354	39.0	37.0	39.0	33.0	39.0
12	36.75799516340785	39.0	35.0	39.0	32.0	39.0
13	36.698112548855235	39.0	35.0	39.0	32.0	39.0
14	37.793589184268214	40.0	37.0	41.0	33.0	41.0
15	37.956313685231414	40.0	37.0	41.0	33.0	41.0
16	37.999162143857646	40.0	37.0	41.0	33.0	41.0
17	37.92276317748383	40.0	36.0	41.0	33.0	41.0
18	37.7648595025612	39.0	36.0	41.0	33.0	41.0
19	37.67711772081507	39.0	36.0	41.0	33.0	41.0
20	37.494112146143735	39.0	35.0	41.0	33.0	41.0
21	37.38061056739303	39.0	35.0	41.0	32.0	41.0
22	37.334181399908964	39.0	35.0	41.0	32.0	41.0
23	37.24867232459808	39.0	35.0	41.0	32.0	41.0
24	37.17871426470353	39.0	35.0	41.0	32.0	41.0
25	37.08518361775021	39.0	35.0	41.0	32.0	41.0
26	37.07449216795953	39.0	35.0	41.0	32.0	41.0
27	37.036175565096805	39.0	35.0	41.0	32.0	41.0
28	36.95676279414212	39.0	35.0	41.0	32.0	41.0
29	36.882258463754724	39.0	35.0	41.0	31.0	41.0
30	36.73925601527781	39.0	35.0	41.0	31.0	41.0
31	36.55545560342197	39.0	35.0	41.0	31.0	41.0
32	36.31549405471246	39.0	35.0	41.0	30.0	41.0
33	36.07707578296193	39.0	35.0	41.0	29.0	41.0
34	35.869994907546406	39.0	35.0	41.0	28.0	41.0
35	35.66597648642324	39.0	35.0	41.0	27.0	41.0
36	35.59957638714179	38.0	35.0	41.0	26.0	41.0
37	35.54732209239706	38.0	35.0	41.0	26.0	41.0
38	35.424429767947636	38.0	35.0	41.0	25.0	41.0
39	35.34616454458577	38.0	35.0	41.0	25.0	41.0
40	35.28185705858889	38.0	35.0	41.0	24.0	41.0
41	35.20106295115006	38.0	35.0	40.0	24.0	41.0
42	35.187273460703985	38.0	35.0	40.0	24.0	41.0
43	35.16319140305543	38.0	34.0	40.0	24.0	41.0
44	35.04737558455669	38.0	34.0	40.0	24.0	41.0
45	34.99292371927945	38.0	34.0	40.0	24.0	41.0
46	34.96807565390505	38.0	34.0	40.0	23.0	41.0
47	34.91091562135479	38.0	34.0	40.0	23.0	41.0
48	34.80479866339422	38.0	34.0	40.0	23.0	41.0
49	34.730519788382765	38.0	34.0	40.0	24.0	41.0
50	34.647047469280295	38.0	34.0	40.0	23.0	41.0
51	34.24805947337138	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	7.0
12	11.0
13	18.0
14	36.0
15	96.0
16	251.0
17	630.0
18	1495.0
19	2994.0
20	5483.0
21	9064.0
22	14148.0
23	21807.0
24	33945.0
25	54929.0
26	75347.0
27	85258.0
28	86474.0
29	89793.0
30	100302.0
31	119712.0
32	146462.0
33	185468.0
34	276197.0
35	370071.0
36	353294.0
37	439182.0
38	651980.0
39	1315022.0
40	422.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.08820237739428	23.268323654818584	29.109554870905963	17.53391909688117
2	34.75064657944104	24.44774131326743	28.56548893072664	12.236123176564893
3	27.666505326805563	24.159131404447347	35.69602759339563	12.478335675351467
4	25.375757082981316	26.327985093368028	34.82648156952978	13.469776254120866
5	22.743672553206682	30.285031001803418	33.14696289536055	13.82433354962935
6	20.478532976959183	39.01348745682057	29.953334566092998	10.554645000127255
7	83.54198278656088	2.660981557479972	11.62998380820482	2.167051847754332
8	83.5331087188166	2.381223193389585	11.338896367781008	2.746771720012802
9	78.11159387941584	5.406199189486798	13.192112530386362	3.2900944007110065
10	34.4734558390127	38.41534375863617	17.781064135860625	9.330136266490506
11	22.54666374468091	23.80306506696205	35.79902533906709	17.851245849289953
12	23.69957181496983	22.01951258845069	35.76472278786271	18.516192808716767
13	22.682905459871535	23.247287159201452	36.133064168293764	17.93674321263325
14	16.19636735307055	28.38476426174175	35.80445338558072	19.614414999606975
15	15.002985425582496	30.2273946074948	38.16961766957521	16.600002297347487
16	20.812459191112957	28.052684934783485	35.86546823207624	15.269387642027315
17	20.339025424654547	27.921803697062753	32.52221951695792	19.216951361324785
18	18.892011829988178	28.647044766518547	34.93828581390179	17.522657589591482
19	20.090709188916968	29.71664020587837	32.17329297509428	18.019357630110388
20	20.613400788260464	30.20984917913747	31.839501899027972	17.33724813357409
21	21.608377480318826	27.210436804587847	35.06637419781468	16.114811517278643
22	19.87151521103051	24.04133603819723	35.485977959428396	20.601170791343865
23	16.920865163045228	28.38321017373578	35.524199515169585	19.171725148049408
24	17.405267637603792	28.965137301423027	35.725037236173854	17.904557824799326
25	19.263033449154182	29.996218385852124	32.0689888945772	18.671759270416494
26	17.72520705970378	32.059281475293496	30.750041160809143	19.465470304193584
27	17.156861309807894	30.422961943087497	34.43068463432647	17.989492112778137
28	16.66164778825123	29.270526856104738	35.26779751719801	18.80002783844602
29	17.570316288441436	29.039846140782803	34.30919549368534	19.080642077090424
30	19.492993428009576	28.396566321381343	33.64199623280058	18.468444017808498
31	20.414905460772452	28.497897363973944	32.037253967034864	19.04994320821874
32	20.627094781124722	29.685558445758836	32.01846977287567	17.66887700024077
33	20.47465901845153	28.184422047058234	32.63627606278786	18.704642871702376
34	19.09255675180291	28.38746702349128	33.109191799910946	19.410784424794866
35	18.62547447545588	28.759209379123824	32.621253212063415	19.994062933356876
36	21.099537534941643	28.695739524039148	32.50332270772582	17.701400233293384
37	18.61335709361218	29.657404677534622	32.69251603019255	19.036722198660648
38	19.052916246143216	30.000520281636785	31.682133596161897	19.264429876058102
39	18.667389805588094	28.60823761239829	31.85150666579878	20.872865916214835
40	19.965098336607802	27.8648655162061	32.31987275397683	19.850163393209268
41	17.715702347551286	28.28933424896895	32.45203780352859	21.54292559995117
42	18.693921916762594	29.657967752899104	32.08666946102201	19.56144086931629
43	19.170621520335015	28.718825613983007	32.397712292363146	19.712840573318832
44	19.828203453994377	29.302149168574175	31.039011437862495	19.83063593956895
45	18.89401637828574	29.139217681107	30.270345996297664	21.696419944309593
46	20.389679684443557	28.790786645564104	30.631119643830058	20.18841402616228
47	18.96663057728964	28.999620036744044	31.95333321471212	20.080416171254193
48	18.463488954601033	29.05356265666164	32.25795698689814	20.224991401839183
49	19.60887433802045	27.915384637907632	31.858150955099696	20.617590068972227
50	17.963455507924383	28.36503410097022	32.78990554523376	20.88160484587163
51	17.353915164362824	28.653846716921517	32.30455710406286	21.6876810146528
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3382.0
1	5295.5
2	7209.0
3	61090.5
4	114972.0
5	76105.0
6	37238.0
7	36597.0
8	35956.0
9	38660.5
10	41365.0
11	44081.0
12	46797.0
13	47733.0
14	48669.0
15	46898.0
16	45127.0
17	43320.5
18	41514.0
19	40112.0
20	38710.0
21	38282.0
22	37854.0
23	38866.0
24	39878.0
25	46614.5
26	60219.5
27	67088.0
28	77007.5
29	86927.0
30	99180.0
31	111433.0
32	125676.0
33	139919.0
34	154593.0
35	169267.0
36	181802.0
37	194337.0
38	205856.5
39	217376.0
40	241552.0
41	265728.0
42	290258.0
43	314788.0
44	350504.0
45	386220.0
46	395683.0
47	405146.0
48	389193.5
49	373241.0
50	331655.0
51	290069.0
52	253995.0
53	217921.0
54	193901.0
55	169881.0
56	153365.0
57	136849.0
58	122607.5
59	108366.0
60	94175.0
61	79984.0
62	65093.0
63	50202.0
64	39717.5
65	29233.0
66	22560.0
67	15887.0
68	12161.5
69	8436.0
70	6632.5
71	4829.0
72	3483.0
73	2137.0
74	1635.5
75	938.5
76	743.0
77	591.5
78	440.0
79	272.5
80	105.0
81	94.0
82	83.0
83	72.5
84	62.0
85	48.5
86	35.0
87	21.0
88	7.0
89	6.0
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4439903.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.064278900812383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.21031084139584	15.631866848791429
2	9.407272749097695	3.9631483376601175
3	3.780998649506044	2.3893203023037084
4	2.0993995337894824	1.768893492159086
5	1.4091059691599959	1.4840900567592843
6	0.9948616326788324	1.2573625739078693
7	0.7548625090225215	1.1130444095272176
8	0.617177174265125	1.0400313703948703
9	0.4809601016807748	0.9117969949770232
>10	4.252522334321337	19.72961050493417
>50	1.1319359960882496	16.998470060426538
>100	0.8515940878656458	29.05313721525875
>500	0.00531232090713153	0.7418755502075561
>1k	0.0030356119469197454	1.3326629812156878
>5k	5.420735619499545E-4	0.7080771196719038
>10k+	1.084147123899909E-4	1.8766121818047699
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82175	1.8508287230599405	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT	7522	0.16941811566604045	No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	6565	0.1478635907135809	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCT	5970	0.1344623970388542	No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	5549	0.12498020790093839	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	5400	0.12162427872861187	No Hit
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	4654	0.10482210985239993	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15874220675541786	0.0
2	0.0	0.0	0.0	0.44759986873587104	0.0
3	0.0	0.0	0.0	0.7037541135470753	0.0
4	0.0	0.0	0.0	0.9955622904374263	0.0
5	0.0	0.0	0.0	1.6884828339718232	0.0
6	0.0	0.0	0.0	2.6704412236033086	0.0
7	0.0	0.0	0.0	3.3853667523817523	0.0
8	0.0	0.0	0.0	4.566293452807415	0.0
9	0.0	0.0	0.0	5.189798065408186	0.0
10	0.0	0.0	0.0	5.918124787861356	0.0
11	0.0	0.0	0.0	6.734156129086604	0.0
12	0.0	0.0	0.0	7.3517597118675795	0.0
13	0.0	0.0	0.0	7.6444462863265255	0.0
14	0.0	0.0	0.0	7.820283461147687	0.0
15	0.0	0.0	0.0	7.997945000149778	0.0
16	0.0	0.0	0.0	8.28612697169285	0.0
17	0.0	0.0	0.0	8.656405331377735	0.0
18	0.0	0.0	0.0	9.073081101096127	0.0
19	0.0	0.0	0.0	9.363650512184613	0.0
20	0.0	0.0	0.0	9.666540012248015	0.0
21	0.0	0.0	0.0	10.000713979562166	0.0
22	0.0	0.0	0.0	10.379213239568521	0.0
23	0.0	0.0	0.0	10.76196934933038	0.0
24	0.0	0.0	0.0	11.056705518116049	0.0
25	0.0	0.0	0.0	11.313512930350054	0.0
26	0.0	0.0	0.0	11.566468907090988	0.0
27	0.0	0.0	0.0	11.834087366323093	0.0
28	0.0	0.0	0.0	12.117989965096085	0.0
29	0.0	0.0	0.0	12.428086829824887	0.0
30	0.0	0.0	0.0	12.787734326628307	0.0
31	2.2523014579372568E-5	0.0	0.0	13.097696053269631	0.0
32	2.2523014579372568E-5	0.0	0.0	13.383715815413085	0.0
33	2.2523014579372568E-5	0.0	0.0	13.67502848598269	0.0
34	2.2523014579372568E-5	0.0	0.0	13.977467525754504	0.0
35	2.2523014579372568E-5	0.0	0.0	14.302632287236905	0.0
36	2.2523014579372568E-5	0.0	0.0	14.607841657801984	0.0
37	2.2523014579372568E-5	0.0	0.0	14.925889146677303	0.0
38	2.2523014579372568E-5	0.0	0.0	15.280851856448216	0.0
39	2.2523014579372568E-5	0.0	0.0	15.783880864063923	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGAC	40	6.8230293E-9	45.000004	4
CGTTTTT	34605	0.0	43.784134	1
CGACGGT	960	0.0	42.656254	28
TACGGGA	2325	0.0	41.03226	4
CACGACG	1020	0.0	40.14706	26
ACGGGAT	2960	0.0	39.98311	5
GGCGATA	700	0.0	39.857143	8
TCACGAC	1055	0.0	39.668243	25
TACGACG	125	0.0	39.6	1
CGTTAGG	660	0.0	39.204548	2
AGGGATC	6265	0.0	39.07422	6
CGAGGGA	3235	0.0	39.01855	4
TTGCGAG	375	0.0	39.0	1
CTCACGA	1085	0.0	38.7788	24
TAGGGAT	8215	0.0	38.727936	5
TAACGGG	1205	0.0	38.65145	3
GGCACCG	760	0.0	38.486843	8
CGGGATT	2785	0.0	38.375225	6
GACGGTC	1075	0.0	38.093025	29
CGGTCTA	1085	0.0	37.94931	31
>>END_MODULE