FastQCFastQC Report
Thu 26 May 2016
SRR1547547_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547547_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2494963
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104910.42048719760573605No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC55500.22244818861041227No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG39010.15635502410256186No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC34040.1364348890143862No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG28910.11587346185093726No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG26420.10589335392949716No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCAA207.034876E-445.00000440
CGCAATC406.8212103E-945.00000420
TTCGATC207.034876E-445.00000434
ATCTCGC253.8921236E-545.011
CGTGATA253.8921236E-545.035
CGTCTAG253.8921236E-545.020
TAAGTCG351.212702E-745.033
TCGATCT253.8921236E-545.035
ATCGTAC502.1827873E-1145.010
CCCGTCG351.212702E-745.017
CTAACCG351.212702E-745.02
TATTCGC253.8921236E-545.018
CCGTTGT253.8921236E-545.033
TACGTAA351.212702E-745.041
CTTACGT351.212702E-745.036
ACGCATG600.044.9999961
TACGACG302.1665292E-644.9999961
TTACCGT302.1665292E-644.99999638
ATCGCTA302.1665292E-644.99999610
TACCGAT302.1665292E-644.99999639