FastQCFastQC Report
Thu 26 May 2016
SRR1547546_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547546_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2956595
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202760.6857888889076793No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG900.045.0000041
TACGCGC351.212793E-745.01
TCGCTAA207.0350897E-445.017
CATACCG406.8212103E-945.019
CGACTTA253.8922994E-545.035
TCGACTC207.0350897E-445.044
ATCGATC302.1666656E-644.99999631
CGTTTTT101350.042.5135651
CTCACGA7100.041.51408424
CGACGGT6950.041.4388528
GTCCGCG451.9301297E-840.00000410
TCGGTCA451.9301297E-840.00000439
TCACGAC7300.039.76027325
GGGCGAT26050.039.7312857
CGGTCTA6950.039.49640331
TATAGCG1850.038.918921
CACGACG7300.038.83561726
TACGGGA16300.038.7883454
CGTATAC356.2516247E-638.57142620
TCGCGGA356.2516247E-638.57142629
Produced by FastQC (version 0.11.5)