##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547546_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2956595
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.432478915779807	31.0	31.0	33.0	30.0	34.0
2	31.85567553215777	31.0	31.0	34.0	30.0	34.0
3	31.990976105959728	33.0	31.0	34.0	30.0	34.0
4	35.7243572420301	37.0	35.0	37.0	35.0	37.0
5	35.642687618696506	37.0	35.0	37.0	33.0	37.0
6	35.67820448860936	37.0	35.0	37.0	33.0	37.0
7	35.98740544443862	37.0	35.0	37.0	35.0	37.0
8	35.930627968998124	37.0	35.0	37.0	35.0	37.0
9	37.56640290604564	39.0	37.0	39.0	35.0	39.0
10	37.164350545137225	39.0	37.0	39.0	33.0	39.0
11	36.95415740065853	39.0	37.0	39.0	33.0	39.0
12	36.64272144138781	39.0	35.0	39.0	32.0	39.0
13	36.52844031732449	39.0	35.0	39.0	32.0	39.0
14	37.62196919091049	40.0	36.0	41.0	32.0	41.0
15	37.80742036024549	40.0	36.0	41.0	33.0	41.0
16	37.849055078561655	40.0	36.0	41.0	33.0	41.0
17	37.771834492042366	40.0	36.0	41.0	33.0	41.0
18	37.67390968326741	39.0	36.0	41.0	33.0	41.0
19	37.6570419688865	39.0	36.0	41.0	33.0	41.0
20	37.54558943649705	39.0	35.0	41.0	32.0	41.0
21	37.42934930215332	39.0	35.0	41.0	32.0	41.0
22	37.365586764504435	39.0	35.0	41.0	32.0	41.0
23	37.27302657279743	39.0	35.0	41.0	32.0	41.0
24	37.20385713971646	39.0	35.0	41.0	32.0	41.0
25	37.125757839677064	39.0	35.0	41.0	32.0	41.0
26	37.13785790749156	39.0	35.0	41.0	32.0	41.0
27	37.098601262601065	39.0	35.0	41.0	32.0	41.0
28	37.026159822363226	39.0	35.0	41.0	31.0	41.0
29	36.98123145036774	39.0	35.0	41.0	31.0	41.0
30	36.9043051212628	39.0	35.0	41.0	31.0	41.0
31	36.771336283799435	39.0	35.0	41.0	31.0	41.0
32	36.63612534012944	39.0	35.0	41.0	30.0	41.0
33	36.5104483366846	39.0	35.0	41.0	30.0	41.0
34	36.41827642947377	39.0	35.0	41.0	30.0	41.0
35	36.287577094597	39.0	35.0	41.0	30.0	41.0
36	36.22612397031044	39.0	35.0	41.0	30.0	41.0
37	36.17867952830875	39.0	35.0	41.0	29.0	41.0
38	36.064835731644	39.0	35.0	41.0	29.0	41.0
39	35.997132174004214	39.0	35.0	41.0	29.0	41.0
40	35.94213850730316	39.0	35.0	41.0	29.0	41.0
41	35.87194154086035	39.0	35.0	41.0	28.0	41.0
42	35.84612975399065	38.0	35.0	41.0	28.0	41.0
43	35.819686159247375	38.0	35.0	41.0	28.0	41.0
44	35.721818510820725	38.0	35.0	40.0	28.0	41.0
45	35.66701120714876	38.0	35.0	40.0	28.0	41.0
46	35.6491443704667	38.0	35.0	40.0	28.0	41.0
47	35.58976457715717	38.0	35.0	40.0	28.0	41.0
48	35.47512324143145	38.0	34.0	40.0	27.0	41.0
49	35.4083613075176	38.0	34.0	40.0	27.0	41.0
50	35.31792721018604	38.0	34.0	40.0	27.0	41.0
51	34.90851841391871	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	5.0
12	11.0
13	16.0
14	40.0
15	81.0
16	192.0
17	489.0
18	1124.0
19	2024.0
20	3674.0
21	5696.0
22	8870.0
23	12853.0
24	18510.0
25	27333.0
26	37050.0
27	43557.0
28	47658.0
29	53104.0
30	62536.0
31	76412.0
32	95029.0
33	122629.0
34	196866.0
35	262313.0
36	214540.0
37	284329.0
38	456126.0
39	923252.0
40	271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.61583341648078	23.78289890904909	27.716004390185333	13.885263284284793
2	31.9503685827785	25.44775324317331	29.194123645612606	13.40775452843558
3	29.748545201490227	25.37422271227544	31.197407828938356	13.679824257295978
4	26.88413529752976	27.23440308868817	31.329891310781488	14.55157030300058
5	24.771705289361577	31.232076087526362	29.09925099650104	14.896967626611016
6	22.456000906448125	40.2311780950722	26.606958342282255	10.705862656197416
7	86.93233263263991	3.659209326945354	7.274787382106782	2.133670658307952
8	87.20332003537854	3.4194740909728925	7.202778872317649	2.174427001330923
9	81.1891720036055	6.692428283210923	8.789299853378633	3.3290998598049444
10	37.292831787918196	38.24091564789902	14.64062544920762	9.825627114975166
11	29.327080645134014	23.651227171797288	29.816156761409662	17.20553542165904
12	28.26775395344983	22.193942694214122	31.507866312430345	18.030437039905703
13	25.773026065457056	24.87814529889958	30.45723205241164	18.891596583231724
14	18.575591178365652	30.580312826071882	31.77239358112964	19.07170241443282
15	18.30467142100964	29.691756902788512	35.361623759764186	16.64194791643766
16	23.412709552711817	27.86614331688987	32.91894899369038	15.802198136707938
17	22.64276980783638	27.041749039012785	29.79325879939593	20.52222235375491
18	22.014750075678272	28.007758925385453	31.386747254865817	18.59074374407046
19	23.68400135967219	28.743334815894634	28.986519966380246	18.586143858052928
20	25.253780108537015	29.066544454008753	28.790314534117794	16.88936090333644
21	25.343072013583196	25.884438010616943	31.638354255486462	17.134135720313402
22	23.418729991764174	23.634180535379382	32.42693030327116	20.520159169585284
23	20.864947684752224	26.226858937392507	32.617250587246474	20.29094279060879
24	20.106270896081472	27.870607912142177	32.92679585807322	19.096325333703128
25	20.641075291001982	29.358028407678425	29.904298694951454	20.096597606368135
26	20.50142139860211	31.47810234408162	28.218102242613547	19.80237401470272
27	19.408542597142997	30.331952803816552	31.4609542395898	18.79855035945065
28	18.347592416276157	29.39154669476205	33.15442257055836	19.106438318403434
29	19.7197113571524	28.005053110081025	32.62056521099441	19.654670321772176
30	20.677908201833528	27.604795381173275	32.02146387990239	19.69583253709081
31	22.547322172972624	28.47958546909536	28.965414607005695	20.00767775092632
32	22.81932425645041	29.112509491492748	29.63084223574754	18.4373240163093
33	22.46878588376156	28.363100120239665	29.542125316453554	19.62598867954522
34	20.6330254904713	28.5730713878634	30.405212753183985	20.38869036848131
35	20.596767565391946	28.671766001092475	29.845751616301865	20.88571481721372
36	22.468007961861534	28.30509420465096	29.533162303257633	19.693735530229876
37	20.553880392816737	30.084573639609076	29.833744560888455	19.527801406685732
38	21.116520862681565	30.124247656510278	28.356403227361206	20.402828253446955
39	20.387134524681265	29.194123645612606	29.40798452273646	21.010757306969673
40	21.176860543970342	27.512019738922643	29.9763748501232	21.334744866983808
41	19.984204803160395	27.211708062822265	29.987502515562664	22.816584618454673
42	20.746230038270376	28.49375717675231	29.30387827889853	21.456134506078783
43	21.0695073217671	27.71397502870701	29.570976072137036	21.645541577388855
44	20.41436179118209	29.441266050980943	29.486453166564917	20.657918991272055
45	20.114591278142594	29.888165271198798	28.38735098990562	21.609892460752995
46	21.441726039582694	29.184653292047102	28.832254671336454	20.54136599703375
47	20.592641197052693	29.070670822348006	29.451784907976915	20.88490307262239
48	20.25082907872062	28.639330040130623	30.440726579054623	20.669114302094133
49	20.548502584899182	27.65654409887049	30.43183121124131	21.363122104989017
50	19.70357793339974	28.52720781845332	30.33421892413401	21.434995324012927
51	19.579719237839473	29.456587730142274	29.097018698874887	21.86667433314336
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2043.0
1	3463.0
2	4883.0
3	18813.0
4	32743.0
5	22934.0
6	13125.0
7	13028.5
8	12932.0
9	13578.5
10	14225.0
11	14484.0
12	14743.0
13	14582.5
14	14422.0
15	13361.0
16	12300.0
17	12299.5
18	12299.0
19	13000.0
20	13701.0
21	15076.0
22	16451.0
23	17778.0
24	19105.0
25	24971.5
26	35557.0
27	40276.0
28	49370.5
29	58465.0
30	68882.5
31	79300.0
32	87303.5
33	95307.0
34	107888.0
35	120469.0
36	125844.5
37	131220.0
38	144090.5
39	156961.0
40	170891.5
41	184822.0
42	205332.0
43	225842.0
44	229557.0
45	233272.0
46	238999.0
47	244726.0
48	233427.0
49	222128.0
50	211017.0
51	199906.0
52	183504.5
53	167103.0
54	152965.5
55	138828.0
56	131248.5
57	123669.0
58	109478.5
59	95288.0
60	85466.5
61	75645.0
62	65685.0
63	55725.0
64	46504.5
65	37284.0
66	29915.5
67	22547.0
68	17907.5
69	13268.0
70	10917.0
71	8566.0
72	6891.0
73	5216.0
74	4313.0
75	2674.0
76	1938.0
77	1399.0
78	860.0
79	667.0
80	474.0
81	312.0
82	150.0
83	107.0
84	64.0
85	43.0
86	22.0
87	14.5
88	7.0
89	6.5
90	6.0
91	8.0
92	10.0
93	10.0
94	10.0
95	5.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2956595.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.626256478431813
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.18081698659807	12.998543149125187
2	8.314942480389291	2.764927325647207
3	2.8605018277970524	1.4267831113793037
4	1.460629021984781	0.9713917095743997
5	0.8771932741952239	0.7292220178962577
6	0.6126811390854242	0.611195625475921
7	0.42849605269787794	0.4986999690505387
8	0.3443797347924423	0.45805966373067797
9	0.28801059075965146	0.43096841554271664
>10	3.3046236000229134	13.356166485464247
>50	1.5986180723468877	19.494648684830036
>100	1.7220867234618207	44.481644387849514
>500	0.004336188624704096	0.5116998941417045
>1k	0.0024778220712594837	0.5699591843368385
>5k	0.0	0.0
>10k+	2.0648517260495695E-4	0.6960903759555317
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20276	0.6857888889076793	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.764538261073972E-5	0.0	0.0	0.04552534249702783	0.0
2	6.764538261073972E-5	0.0	0.0	0.13806422590851977	0.0
3	6.764538261073972E-5	0.0	0.0	0.19931711986254458	0.0
4	6.764538261073972E-5	0.0	0.0	0.29821466923944606	0.0
5	6.764538261073972E-5	0.0	0.0	0.5393028128641224	0.0
6	6.764538261073972E-5	0.0	0.0	0.7624311074056473	0.0
7	6.764538261073972E-5	0.0	0.0	0.91859047316254	0.0
8	6.764538261073972E-5	0.0	0.0	1.2402780901679127	0.0
9	6.764538261073972E-5	0.0	0.0	1.36163390657158	0.0
10	6.764538261073972E-5	0.0	0.0	1.6239965230273339	0.0
11	6.764538261073972E-5	0.0	0.0	1.9089526972750748	0.0
12	6.764538261073972E-5	0.0	0.0	2.168372739587262	0.0
13	6.764538261073972E-5	0.0	0.0	2.2717010615251665	0.0
14	6.764538261073972E-5	0.0	0.0	2.320304268930983	0.0
15	6.764538261073972E-5	0.0	0.0	2.3939362679027734	0.0
16	6.764538261073972E-5	0.0	0.0	2.527603543941595	0.0
17	6.764538261073972E-5	0.0	0.0	2.691542128698723	0.0
18	6.764538261073972E-5	0.0	0.0	2.871512669134596	0.0
19	6.764538261073972E-5	0.0	0.0	3.0053828813212498	0.0
20	6.764538261073972E-5	0.0	0.0	3.139083980051377	0.0
21	6.764538261073972E-5	0.0	0.0	3.2962580265474304	0.0
22	6.764538261073972E-5	0.0	0.0	3.482147537961743	0.0
23	6.764538261073972E-5	0.0	0.0	3.6620166103237	0.0
24	6.764538261073972E-5	0.0	0.0	3.805763048371522	0.0
25	6.764538261073972E-5	0.0	0.0	3.9334775307405985	0.0
26	6.764538261073972E-5	0.0	0.0	4.0619699350096985	0.0
27	6.764538261073972E-5	0.0	0.0	4.1969562960094295	0.0
28	1.0146807391610957E-4	0.0	0.0	4.340736556748557	0.0
29	1.0146807391610957E-4	0.0	0.0	4.5105941124841245	0.0
30	1.0146807391610957E-4	0.0	0.0	4.692052851337434	0.0
31	1.0146807391610957E-4	0.0	0.0	4.860083981742512	0.0
32	1.0146807391610957E-4	0.0	0.0	5.026559268347541	0.0
33	1.0146807391610957E-4	0.0	0.0	5.1897537538959515	0.0
34	1.0146807391610957E-4	0.0	0.0	5.365022940240378	0.0
35	1.0146807391610957E-4	0.0	0.0	5.551893309702546	0.0
36	1.0146807391610957E-4	0.0	0.0	5.743194451725718	0.0
37	1.0146807391610957E-4	0.0	0.0	5.939129302457726	0.0
38	1.3529076522147943E-4	0.0	0.0	6.153666633407687	0.0
39	1.3529076522147943E-4	0.0	0.0	6.4857039939525025	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCACG	90	0.0	45.000004	1
TACGCGC	35	1.212793E-7	45.0	1
TCGCTAA	20	7.0350897E-4	45.0	17
CATACCG	40	6.8212103E-9	45.0	19
CGACTTA	25	3.8922994E-5	45.0	35
TCGACTC	20	7.0350897E-4	45.0	44
ATCGATC	30	2.1666656E-6	44.999996	31
CGTTTTT	10135	0.0	42.513565	1
CTCACGA	710	0.0	41.514084	24
CGACGGT	695	0.0	41.43885	28
GTCCGCG	45	1.9301297E-8	40.000004	10
TCGGTCA	45	1.9301297E-8	40.000004	39
TCACGAC	730	0.0	39.760273	25
GGGCGAT	2605	0.0	39.731285	7
CGGTCTA	695	0.0	39.496403	31
TATAGCG	185	0.0	38.91892	1
CACGACG	730	0.0	38.835617	26
TACGGGA	1630	0.0	38.788345	4
CGTATAC	35	6.2516247E-6	38.571426	20
TCGCGGA	35	6.2516247E-6	38.571426	29
>>END_MODULE