FastQCFastQC Report
Thu 26 May 2016
SRR1547545_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547545_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4368762
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT674521.5439614243119677No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT70330.16098382104587067No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC50800.11628008117631494No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC46990.10755907508809132No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGA253.892606E-545.00000424
AACGTTC207.0354587E-445.035
CGTTCGA406.8230293E-945.030
CGGTCGC207.0354587E-445.018
CGCACCG351.2129749E-745.031
GCGTAAT207.0354587E-445.031
ACGGTCG207.0354587E-445.017
TACGATC551.8189894E-1244.99999637
TCACGAC15250.043.8196725
CGTTTTT240100.043.6599351
CGACGGT14500.043.6034528
TCTACGG2900.043.4482732
CGGTCTA14450.043.44290531
CACGACG15100.043.2119226
TCGCGAG1100.042.9545441
CGCATCG1000.042.75000421
TACGGGA18550.042.4528274
TGCGTAG3050.042.0491831
CGCCGAC1150.041.08695613
ACGGTCT15450.040.9223330