FastQCFastQC Report
Thu 26 May 2016
SRR1547544_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547544_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3301312
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141540.4287386348215497No Hit
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT45100.13661235290696547RNA PCR Primer, Index 16 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC37020.1121372351356067Illumina PCR Primer Index 7 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG35720.10819940678130392No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT34440.10432216040168273No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG1350.045.0000041
CCGTTCG351.2128476E-745.00000443
CGCGAAA502.1827873E-1145.029
CGCTAAC207.0352084E-445.026
TAATCCG207.0352084E-445.01
CGGTCTA13550.043.3394831
CGACGGT13800.042.71739228
CACGACG14050.042.1174426
GTTTACG1500.042.01
TACGTAG1500.042.01
ACGGTCT14150.041.81978630
CTCACGA14350.041.55052624
TATAGCG1900.041.447371
TCTCACG14450.041.10726523
CGTAAGT556.184564E-1140.90909224
AGAATCG501.0822987E-940.513
CGTTTTT81200.040.400251
GACGGTC14600.040.3767129
CGACTAG451.9301297E-840.017
TACGGGA14450.039.861594
Produced by FastQC (version 0.11.5)