##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547544_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3301312
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.347232251904696	31.0	31.0	33.0	30.0	34.0
2	31.759445941492352	31.0	31.0	34.0	30.0	34.0
3	31.907806048019697	31.0	31.0	34.0	30.0	34.0
4	35.65229127086443	37.0	35.0	37.0	33.0	37.0
5	35.551151481592775	37.0	35.0	37.0	33.0	37.0
6	35.59447031967896	37.0	35.0	37.0	33.0	37.0
7	35.92257775090631	37.0	35.0	37.0	35.0	37.0
8	35.85168108921544	37.0	35.0	37.0	35.0	37.0
9	37.49185808551267	39.0	37.0	39.0	35.0	39.0
10	37.075426678847684	39.0	37.0	39.0	33.0	39.0
11	36.85031254240738	39.0	37.0	39.0	32.0	39.0
12	36.418092564410756	39.0	35.0	39.0	32.0	39.0
13	36.24068461266309	39.0	35.0	39.0	32.0	39.0
14	37.23041293885583	39.0	35.0	41.0	32.0	41.0
15	37.43934350948956	39.0	35.0	41.0	32.0	41.0
16	37.5275263289262	40.0	35.0	41.0	32.0	41.0
17	37.43446181396972	39.0	35.0	41.0	32.0	41.0
18	37.37237558885679	39.0	35.0	41.0	32.0	41.0
19	37.36332857966772	39.0	35.0	41.0	32.0	41.0
20	37.25081149555086	39.0	35.0	41.0	32.0	41.0
21	37.15369132029932	39.0	35.0	41.0	32.0	41.0
22	37.10837812360661	39.0	35.0	41.0	32.0	41.0
23	37.016620664753894	39.0	35.0	41.0	32.0	41.0
24	36.95521386648702	39.0	35.0	41.0	31.0	41.0
25	36.87566731045112	39.0	35.0	41.0	31.0	41.0
26	36.897857276137486	39.0	35.0	41.0	31.0	41.0
27	36.862540711086986	39.0	35.0	41.0	31.0	41.0
28	36.80877208818797	39.0	35.0	41.0	31.0	41.0
29	36.75379970145203	39.0	35.0	41.0	31.0	41.0
30	36.649340019967816	38.0	35.0	41.0	31.0	41.0
31	36.513681530252214	38.0	35.0	41.0	30.0	41.0
32	36.39861212754202	38.0	35.0	41.0	30.0	41.0
33	36.29808209584553	38.0	35.0	41.0	30.0	41.0
34	36.189700640230306	38.0	35.0	41.0	30.0	41.0
35	36.11821542465541	38.0	35.0	41.0	30.0	41.0
36	36.041465635480684	38.0	35.0	41.0	29.0	41.0
37	35.99459941986701	38.0	35.0	41.0	29.0	41.0
38	35.873830464978774	38.0	35.0	41.0	29.0	41.0
39	35.808998058953534	38.0	35.0	40.0	29.0	41.0
40	35.70724881501658	38.0	35.0	40.0	28.0	41.0
41	35.63242735009596	38.0	35.0	40.0	28.0	41.0
42	35.63162433602156	38.0	35.0	40.0	28.0	41.0
43	35.58844483647714	38.0	34.0	40.0	28.0	41.0
44	35.48936331979528	38.0	34.0	40.0	27.0	41.0
45	35.42128886939496	38.0	34.0	40.0	27.0	41.0
46	35.37524747736657	38.0	34.0	40.0	27.0	41.0
47	35.30776612449838	38.0	34.0	40.0	27.0	41.0
48	35.22450892251323	38.0	34.0	40.0	27.0	41.0
49	35.14545853284997	37.0	34.0	40.0	27.0	41.0
50	35.054994196246824	37.0	34.0	40.0	27.0	41.0
51	34.62905656902468	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	5.0
11	1.0
12	17.0
13	15.0
14	41.0
15	103.0
16	222.0
17	572.0
18	1137.0
19	2154.0
20	3875.0
21	6527.0
22	10031.0
23	14725.0
24	21544.0
25	30643.0
26	40118.0
27	47827.0
28	55252.0
29	64532.0
30	78190.0
31	96493.0
32	119540.0
33	153513.0
34	247703.0
35	331156.0
36	244959.0
37	316614.0
38	486712.0
39	926786.0
40	302.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.04885330438323	23.31921369443421	28.695773074462515	12.936159926720043
2	30.934670821782372	25.96943275885466	29.50790473605645	13.587991683306516
3	29.815752040400906	25.336623742318203	30.85264282806351	13.99498138921738
4	26.899487234166298	27.75302667545509	30.105303588391525	15.24218250198709
5	24.4865677645736	31.305371924858967	28.73772609192951	15.470334218637923
6	22.224921485760813	40.340658501832	26.38169309656282	11.052726915844367
7	86.15765489599286	4.212931101331834	7.244119913537405	2.3852940891378944
8	85.82920972025667	4.339820047302405	6.786726004691468	3.0442442277494526
9	80.41775512281177	6.488935308144156	8.833185109435279	4.260124459608786
10	39.78106280169823	35.475501861078264	14.125141761820755	10.618293575402749
11	32.435225752670455	23.521284870984626	26.927415524494503	17.116073851850416
12	31.17721075742008	21.80490665529341	28.805820231471607	18.2120623558149
13	25.478718763933855	27.193491557296007	28.780466675067366	18.547323003702772
14	17.861686505243977	32.08836365663106	30.254759319930987	19.795190518193976
15	17.636715342263923	29.05069257313456	35.307750373184966	18.004841711416553
16	22.869907479208265	26.92341711416552	33.08448277533296	17.122192631293256
17	22.251426099684004	27.00547539887172	27.4972798693368	23.245818632107476
18	23.370284299090784	27.117612634007326	30.502660760328016	19.00944230657387
19	24.612760017835335	28.988353721187217	26.551928445418067	19.846957815559392
20	25.908032927514878	28.526597910164202	27.311777862861796	18.253591299459124
21	24.124166392028382	28.656213044995443	29.46498240699455	17.754638155981624
22	23.371344483647714	23.844822906771608	30.414635151115675	22.369197458465
23	20.581786877459628	28.463138291685247	30.197509353856887	20.757565476998234
24	20.76571375259291	27.482619031463855	31.38709700870442	20.36457020723882
25	20.860039887172128	29.27530024426652	28.557949082061917	21.306710786499426
26	19.659032530097125	30.72511777135878	26.867621115483782	22.74822858306031
27	19.517997693038406	30.406456584533665	29.614226101622627	20.461319620805305
28	17.25147456526375	29.068746001589673	32.17623781090669	21.503541622239887
29	19.70637734331078	27.97369651823275	31.143587761471803	21.176338376984667
30	21.624493534691663	28.669601661400073	30.041692514975864	19.6642122889324
31	24.627420855708277	28.371174854118603	26.222029302289513	20.779374987883607
32	24.561780286140785	28.166559234631567	26.89330787274877	20.378352606478877
33	23.825466965860844	27.65364194598996	26.42924994668786	22.091641141461334
34	19.18216151639106	28.49909369365876	30.049659044646493	22.269085745303684
35	20.093829362386835	28.54416668282186	28.24149913731268	23.120504817478626
36	23.543033799895312	28.220507483085512	26.971579784037374	21.264878932981794
37	20.579363598472362	29.92319417249869	27.967608029777253	21.52983419925169
38	20.419154566426922	29.913955421359752	26.257560630440263	23.409329381773063
39	20.288236919140026	28.135935046430028	27.04906412965512	24.52676390477483
40	22.634213306709576	27.2972987709129	27.2958145125332	22.77267340984433
41	19.18912844347944	26.485136818331622	28.409462662117363	25.91627207607157
42	21.31516197196751	26.810249985460327	27.28318317081209	24.591404871760076
43	21.572817110288273	26.73100876257682	27.85416828218599	23.84200584494892
44	20.844652065603007	28.219810790376677	27.733882771455715	23.201654372564605
45	20.35733066126437	29.002560194250044	26.230541069732276	24.40956807475331
46	21.40942752457205	29.101248231006338	27.162382713297017	22.326941531124593
47	19.607659015567144	27.590394364422387	29.4215754221352	23.38037119787527
48	20.4856735746273	27.21542223213074	29.51468991722079	22.78421427602117
49	21.40370252796464	26.08187290386368	28.998016546148925	23.51640802202276
50	18.845416610123493	27.359486167923542	30.361171558459183	23.43392566349379
51	18.83787415427563	27.783408535757903	28.085954917317725	25.292762392648743
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2320.0
1	3711.0
2	5102.0
3	14648.0
4	24194.0
5	17360.0
6	10526.0
7	10823.5
8	11121.0
9	12240.0
10	13359.0
11	13585.0
12	13811.0
13	14019.5
14	14228.0
15	13257.5
16	12287.0
17	12108.5
18	11930.0
19	11644.5
20	11359.0
21	12877.5
22	14396.0
23	14468.5
24	14541.0
25	19387.5
26	30058.0
27	35882.0
28	40949.0
29	46016.0
30	56409.5
31	66803.0
32	76524.5
33	86246.0
34	98798.0
35	111350.0
36	122482.0
37	133614.0
38	139403.0
39	145192.0
40	172652.5
41	200113.0
42	209504.0
43	218895.0
44	231649.0
45	244403.0
46	247771.5
47	251140.0
48	272494.0
49	293848.0
50	289892.0
51	285936.0
52	270374.5
53	254813.0
54	221940.5
55	189068.0
56	174857.0
57	160646.0
58	144811.5
59	128977.0
60	113538.5
61	98100.0
62	82133.0
63	66166.0
64	54836.0
65	43506.0
66	33491.0
67	23476.0
68	18657.5
69	13839.0
70	11827.0
71	9815.0
72	7225.5
73	4636.0
74	3578.5
75	2016.0
76	1511.0
77	1067.0
78	623.0
79	540.0
80	457.0
81	301.0
82	145.0
83	119.0
84	93.0
85	64.5
86	36.0
87	26.0
88	16.0
89	14.0
90	12.0
91	7.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3301312.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.48433487015219
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.69564207893401	13.060036194491131
2	10.453846270804748	3.6555709775968377
3	3.986402327448968	2.0909877966081547
4	2.063307601745337	1.4430224419618434
5	1.2397824937575868	1.0838386143505008
6	0.8349058849960955	0.8758664446999502
7	0.5529454940745392	0.6767518928338697
8	0.4200308780297695	0.5875168421821233
9	0.3442734733626075	0.5417453425664024
>10	2.8294363220368766	10.909661588982422
>50	0.9937204077886471	12.764508644816837
>100	1.572161271814008	49.254689041189636
>500	0.008795776108279637	1.0327191580470871
>1k	0.004573803576305411	1.5877411144135303
>5k	0.0	0.0
>10k+	1.7591552216559273E-4	0.4353439052596639
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14154	0.4287386348215497	No Hit
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	4510	0.13661235290696547	RNA PCR Primer, Index 16 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	3702	0.1121372351356067	Illumina PCR Primer Index 7 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG	3572	0.10819940678130392	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3444	0.10432216040168273	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.029098734079057E-5	0.0	0.0	0.15178813756470155	0.0
2	3.029098734079057E-5	0.0	0.0	0.46205872089641936	0.0
3	3.029098734079057E-5	0.0	0.0	0.6596165403330555	0.0
4	3.029098734079057E-5	0.0	0.0	0.9542266832095846	0.0
5	3.029098734079057E-5	0.0	0.0	1.6246268150359615	0.0
6	3.029098734079057E-5	0.0	0.0	2.1985198611945798	0.0
7	3.029098734079057E-5	0.0	0.0	2.6046917104472405	0.0
8	3.029098734079057E-5	0.0	0.0	3.478677568191071	0.0
9	3.029098734079057E-5	0.0	0.0	3.7682291161816877	0.0
10	3.029098734079057E-5	0.0	0.0	4.380864335149177	0.0
11	3.029098734079057E-5	0.0	0.0	5.074346199329236	0.0
12	3.029098734079057E-5	0.0	0.0	5.7434741096873	0.0
13	3.029098734079057E-5	0.0	0.0	5.982106507958048	0.0
14	3.029098734079057E-5	0.0	0.0	6.1073597406122175	0.0
15	3.029098734079057E-5	0.0	0.0	6.283562413973596	0.0
16	3.029098734079057E-5	0.0	0.0	6.60634317507706	0.0
17	3.029098734079057E-5	0.0	0.0	6.978255917647287	0.0
18	3.029098734079057E-5	0.0	0.0	7.407206589380222	0.0
19	3.029098734079057E-5	0.0	0.0	7.6848234883585675	0.0
20	3.029098734079057E-5	0.0	0.0	7.967135490374736	0.0
21	3.029098734079057E-5	0.0	0.0	8.293854079832503	0.0
22	3.029098734079057E-5	0.0	0.0	8.641715778454142	0.0
23	3.029098734079057E-5	0.0	0.0	8.99684731403757	0.0
24	3.029098734079057E-5	0.0	0.0	9.270920167497044	0.0
25	3.029098734079057E-5	0.0	0.0	9.511794098831011	0.0
26	3.029098734079057E-5	0.0	0.0	9.751335226721983	0.0
27	3.029098734079057E-5	0.0	0.0	10.014139832890681	0.0
28	3.029098734079057E-5	0.0	0.0	10.274369705135413	0.0
29	3.029098734079057E-5	0.0	0.0	10.570252069480254	0.0
30	3.029098734079057E-5	0.0	0.0	10.901968671849252	0.0
31	3.029098734079057E-5	0.0	0.0	11.18573464125778	0.0
32	6.058197468158114E-5	0.0	0.0	11.452143874920031	0.0
33	6.058197468158114E-5	0.0	0.0	11.72870058934145	0.0
34	6.058197468158114E-5	0.0	0.0	12.027854380319097	0.0
35	6.058197468158114E-5	0.0	0.0	12.358813708004575	0.0
36	6.058197468158114E-5	0.0	0.0	12.639399123742319	0.0
37	6.058197468158114E-5	0.0	0.0	12.941703177403408	0.0
38	6.058197468158114E-5	0.0	0.0	13.300348467518369	0.0
39	6.058197468158114E-5	0.0	0.0	13.83531759494407	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	135	0.0	45.000004	1
CCGTTCG	35	1.2128476E-7	45.000004	43
CGCGAAA	50	2.1827873E-11	45.0	29
CGCTAAC	20	7.0352084E-4	45.0	26
TAATCCG	20	7.0352084E-4	45.0	1
CGGTCTA	1355	0.0	43.33948	31
CGACGGT	1380	0.0	42.717392	28
CACGACG	1405	0.0	42.11744	26
GTTTACG	150	0.0	42.0	1
TACGTAG	150	0.0	42.0	1
ACGGTCT	1415	0.0	41.819786	30
CTCACGA	1435	0.0	41.550526	24
TATAGCG	190	0.0	41.44737	1
TCTCACG	1445	0.0	41.107265	23
CGTAAGT	55	6.184564E-11	40.909092	24
AGAATCG	50	1.0822987E-9	40.5	13
CGTTTTT	8120	0.0	40.40025	1
GACGGTC	1460	0.0	40.37671	29
CGACTAG	45	1.9301297E-8	40.0	17
TACGGGA	1445	0.0	39.86159	4
>>END_MODULE