FastQCFastQC Report
Thu 26 May 2016
SRR1547543_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547543_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1468628
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68940.4694177150374363No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC57970.39472214883551177No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20620.1404031517852036No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT18860.12841917762700972No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT15640.10649395217849586Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTGC453.8562575E-1045.00000416
GTCGCAC453.8562575E-1045.00000413
TCGCACC453.8562575E-1045.00000426
GGTCGAT351.2122291E-745.0000048
CGACGGT7200.045.00000428
GTTTACG900.045.0000041
TAGGTGC453.8562575E-1045.0000045
CGTTGAT351.2122291E-745.00000425
CTCCCGA351.2122291E-745.00000443
GCGCATC453.8562575E-1045.00000421
ATCGTCC650.045.00000422
AGTGCGC351.2122291E-745.00000435
AGGTTCC453.8562575E-1045.00000424
CGGATAG453.8562575E-1045.00000426
TACGAGT453.8562575E-1045.00000433
TCGTCCT650.045.00000423
TCGTCCA351.2122291E-745.00000421
TTGCGCG650.045.00000415
CGGTGAT453.8562575E-1045.00000432
TCTCCCG351.2122291E-745.00000442