##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547543_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1468628
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.387610068717198	31.0	31.0	33.0	30.0	34.0
2	31.786032950481673	31.0	31.0	34.0	30.0	34.0
3	31.913786200453757	31.0	31.0	34.0	30.0	34.0
4	35.64306277695918	37.0	35.0	37.0	33.0	37.0
5	35.53100376678097	37.0	35.0	37.0	33.0	37.0
6	35.59006637487505	37.0	35.0	37.0	33.0	37.0
7	35.956692232478204	37.0	35.0	37.0	35.0	37.0
8	35.91531688078942	37.0	35.0	37.0	35.0	37.0
9	37.5996923659361	39.0	37.0	39.0	35.0	39.0
10	37.1250602603246	39.0	37.0	39.0	33.0	39.0
11	36.80708797598847	39.0	35.0	39.0	32.0	39.0
12	36.01758784389239	37.0	35.0	39.0	32.0	39.0
13	35.638494567718986	37.0	35.0	39.0	31.0	39.0
14	36.67607590213451	38.0	35.0	41.0	31.0	41.0
15	36.98743316891684	38.0	35.0	41.0	32.0	41.0
16	37.13816568933726	38.0	35.0	41.0	32.0	41.0
17	37.09210705502006	38.0	35.0	41.0	32.0	41.0
18	37.038207088520714	38.0	35.0	41.0	32.0	41.0
19	36.97760835282999	38.0	35.0	41.0	32.0	41.0
20	36.826469330558865	38.0	35.0	41.0	32.0	41.0
21	36.691669367600234	38.0	35.0	41.0	32.0	41.0
22	36.60812064048895	38.0	35.0	41.0	31.0	41.0
23	36.581277219282214	38.0	35.0	40.0	31.0	41.0
24	36.49190741290511	38.0	35.0	40.0	31.0	41.0
25	36.40578689770316	38.0	35.0	40.0	31.0	41.0
26	36.37756055311488	38.0	35.0	40.0	31.0	41.0
27	36.347700030232296	38.0	35.0	40.0	31.0	41.0
28	36.326587127577575	38.0	35.0	40.0	31.0	41.0
29	36.32208973273014	38.0	35.0	40.0	31.0	41.0
30	36.264047124254745	38.0	35.0	40.0	31.0	41.0
31	36.07676756809757	37.0	35.0	40.0	30.0	41.0
32	35.87372363866139	37.0	35.0	40.0	30.0	41.0
33	35.769326201052955	38.0	35.0	40.0	30.0	41.0
34	35.70120207431698	37.0	35.0	40.0	30.0	41.0
35	35.589446068030846	37.0	35.0	40.0	29.0	41.0
36	35.482690647325256	37.0	35.0	40.0	29.0	41.0
37	35.43710456289816	37.0	34.0	40.0	29.0	41.0
38	35.36090895720359	37.0	34.0	40.0	29.0	41.0
39	35.31562179122283	37.0	34.0	40.0	28.0	41.0
40	35.211344874263595	37.0	34.0	40.0	27.0	41.0
41	35.19783430521548	37.0	34.0	40.0	28.0	41.0
42	35.16530258172934	37.0	34.0	40.0	27.0	41.0
43	35.080522773636346	37.0	34.0	40.0	27.0	41.0
44	34.95294247420041	36.0	34.0	40.0	27.0	41.0
45	34.87144055540273	36.0	34.0	40.0	27.0	41.0
46	34.77283696075521	36.0	34.0	40.0	26.0	41.0
47	34.70683726580182	36.0	34.0	40.0	26.0	41.0
48	34.65554381368189	36.0	34.0	40.0	26.0	41.0
49	34.62824282255275	36.0	34.0	40.0	26.0	41.0
50	34.50327584657245	36.0	34.0	40.0	26.0	41.0
51	34.02086777591058	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	0.0
13	6.0
14	3.0
15	20.0
16	78.0
17	204.0
18	478.0
19	996.0
20	1746.0
21	2980.0
22	4775.0
23	7131.0
24	10577.0
25	15281.0
26	20017.0
27	23282.0
28	25870.0
29	30182.0
30	36221.0
31	44385.0
32	56188.0
33	74641.0
34	134506.0
35	222182.0
36	100841.0
37	121675.0
38	185916.0
39	348292.0
40	149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.46363272387562	24.375539619290933	31.36893753898196	11.791890117851493
2	30.554640113085135	26.55880182047462	29.58754701667134	13.2990110497689
3	28.772296320102846	25.93522661967496	31.497969533469334	13.794507526752861
4	26.80801401035524	27.440645282535808	31.536849358721202	14.214491348387748
5	23.80575612067862	32.44722285017036	30.164275773034422	13.582745256116594
6	21.46731507229877	40.824769785132794	26.562546812399056	11.145368330169383
7	87.08570175701404	4.875366668754784	6.300574413670446	1.7383571605607413
8	87.23897406286683	3.999174739961379	6.099774755758435	2.662076441413346
9	82.71114264469968	6.048638593299324	8.143178531255021	3.0970402307459755
10	49.27558238028963	26.926287664405145	13.784770547749329	10.013359407555896
11	41.74365462186476	23.531418439523147	20.86968245192111	13.85524448669098
12	38.75058898509357	23.36936242533848	23.593244851657467	14.286803737910486
13	21.867620663639805	40.63166438335644	23.408991248975234	14.091723704028523
14	15.856840534158412	41.2670192860275	26.928738931846596	15.947401247967491
15	14.045353894927784	28.429323150586804	43.79373129206307	13.731591662422343
16	15.646031534193819	24.023850832205294	41.49001653243708	18.84010110116381
17	17.04325397581961	26.00665382928829	26.18818380148002	30.761908393412085
18	21.443891850080483	27.968552962356704	31.73975983026335	18.847795357299464
19	26.844578749690186	30.665832327859743	23.23849198027002	19.251096942180048
20	28.59430706754876	27.112175445381677	24.900519396334538	19.392998090735027
21	20.858651748434593	30.90850780456317	27.401901638808468	20.830938808193768
22	21.64544050637738	26.049619100275905	26.181919451351877	26.12302094199484
23	18.45409456989789	31.448603730829046	26.135345369964348	23.961956329308716
24	20.075948436227552	26.751090133103823	35.28633527346612	17.886626157202503
25	16.62681087382237	29.894363991426008	31.55148887260763	21.92733626214399
26	15.684911359445685	38.54543151839676	24.81383985597442	20.955817266183132
27	16.909455627973863	37.0153639995969	29.40220396179291	16.672976410636323
28	14.938977058860377	31.054085854280324	36.385252085620046	17.62168500123925
29	15.029537772669457	28.439877218737486	36.210395008130035	20.32019000046302
30	19.240747146316153	34.28267743771738	29.18227079968515	17.29430461628132
31	28.880832995149213	30.571594712888494	22.90096607173498	17.646606220227316
32	26.122748578945792	32.83615728421356	24.741936011025256	16.299158125815385
33	25.15136576450946	31.342314050937336	23.831902973387407	19.674417211165796
34	19.022924797838527	30.84606857556849	27.595415585158396	22.535591041434593
35	19.783021976974428	30.12798339674853	28.391124232957566	21.69787039331948
36	25.743346851619336	29.706365396819344	27.451539804497806	17.098747947063515
37	19.081210490335195	34.64675874353478	28.192163025626638	18.07986774050338
38	19.373660314252486	34.562939015189684	24.075940265336083	21.987460405221746
39	19.641733645279814	31.933341867375535	28.56945393932296	19.855470548021692
40	23.462510588113535	29.167563195036454	25.949661861274603	21.42026435557541
41	17.29927524192648	27.023793635964992	27.942474200410178	27.734456921698346
42	21.38165689337259	28.656269661207602	24.065726650996712	25.896346794423092
43	21.76834433226113	29.549756643615677	25.53212930708117	23.14976971704203
44	18.856783337918113	32.936046432452606	27.089637403072803	21.117532826556488
45	18.212576636152928	38.47005504457221	22.123233385173098	21.19413493410176
46	22.413231941648938	34.68400439049235	23.92675340521902	18.97601026263969
47	21.151169663114146	30.42744656917885	26.794532039427278	21.62685172827973
48	21.988958401991518	26.998940507739196	30.563355730654735	20.448745359614552
49	20.207159335107324	27.00506867634282	29.827022227548433	22.960749761001427
50	18.836151836952585	31.940355215888573	28.287626274318615	20.935866672840227
51	16.31059737387548	34.53536225647339	26.018773984971006	23.135266384680122
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2385.0
1	2611.0
2	2837.0
3	6647.0
4	10457.0
5	7705.5
6	4954.0
7	5390.0
8	5826.0
9	6931.5
10	8037.0
11	8315.0
12	8593.0
13	8783.0
14	8973.0
15	8543.0
16	8113.0
17	7229.5
18	6346.0
19	6462.0
20	6578.0
21	7000.5
22	7423.0
23	6194.5
24	4966.0
25	7380.0
26	12001.5
27	14209.0
28	16429.5
29	18650.0
30	23832.0
31	29014.0
32	31467.0
33	33920.0
34	40544.0
35	47168.0
36	47962.0
37	48756.0
38	57258.0
39	65760.0
40	86635.0
41	107510.0
42	134105.0
43	160700.0
44	155451.5
45	150203.0
46	152703.5
47	155204.0
48	143280.5
49	131357.0
50	120121.5
51	108886.0
52	98437.0
53	87988.0
54	73975.5
55	59963.0
56	53925.5
57	47888.0
58	43477.0
59	39066.0
60	33467.5
61	27869.0
62	22526.0
63	17183.0
64	13649.5
65	10116.0
66	7619.0
67	5122.0
68	3851.5
69	2581.0
70	2322.0
71	2063.0
72	1417.5
73	772.0
74	661.0
75	487.5
76	425.0
77	359.0
78	293.0
79	189.0
80	85.0
81	56.0
82	27.0
83	17.5
84	8.0
85	7.5
86	7.0
87	3.5
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1468628.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.98258430739446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.08791602849865	14.253626964775314
2	11.00008163088189	4.176199538928733
3	4.200145118689168	2.3918882645642534
4	2.091122623574744	1.5877964599642989
5	1.2879908242088687	1.2224697203847663
6	0.8578403416954585	0.9770415965110867
7	0.5898723596090961	0.7838111257816959
8	0.43738525748263596	0.6642162019980457
9	0.35554803250501266	0.6074298452119167
>10	2.376950087819898	9.248913242443042
>50	0.47814696638178567	6.492813051472468
>100	1.2177294063074295	54.08240993490991
>500	0.01599883364512597	1.9311128307030838
>1k	0.0025452689889973136	0.704306073503977
>5k	7.272197111420895E-4	0.87596514884741
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6894	0.4694177150374363	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5797	0.39472214883551177	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2062	0.1404031517852036	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1886	0.12841917762700972	No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	1564	0.10649395217849586	Illumina Single End Adapter 2 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12787445152890997	0.0
2	0.0	0.0	0.0	0.4206647292575111	0.0
3	0.0	0.0	0.0	0.6074376901434536	0.0
4	0.0	0.0	0.0	0.9108501267849993	0.0
5	0.0	0.0	0.0	1.4881917000084433	0.0
6	0.0	0.0	0.0	2.027879081700744	0.0
7	0.0	0.0	0.0	2.3426626756401214	0.0
8	0.0	0.0	0.0	3.1076623896589197	0.0
9	0.0	0.0	0.0	3.317109574378263	0.0
10	0.0	0.0	0.0	3.796672812992807	0.0
11	0.0	0.0	0.0	4.569502964671789	0.0
12	0.0	0.0	0.0	5.113480064386625	0.0
13	0.0	0.0	0.0	5.330417232954839	0.0
14	0.0	0.0	0.0	5.4200927668545065	0.0
15	0.0	0.0	0.0	5.572547983560167	0.0
16	0.0	0.0	0.0	5.96012060235812	0.0
17	0.0	0.0	0.0	6.41060908548659	0.0
18	0.0	0.0	0.0	6.985839845079898	0.0
19	0.0	0.0	0.0	7.284894472936646	0.0
20	0.0	0.0	0.0	7.575642027797373	0.0
21	0.0	0.0	0.0	7.983369512225016	0.0
22	0.0	0.0	0.0	8.395454805437456	0.0
23	0.0	0.0	0.0	8.907701609937982	0.0
24	0.0	0.0	0.0	9.235354357944967	0.0
25	0.0	0.0	0.0	9.5384263407752	0.0
26	0.0	0.0	0.0	9.812287386594836	0.0
27	0.0	0.0	0.0	10.096974863614204	0.0
28	0.0	0.0	0.0	10.42639797144001	0.0
29	0.0	0.0	0.0	10.762357792443014	0.0
30	0.0	0.0	0.0	11.149385685142867	0.0
31	0.0	0.0	0.0	11.52449769444679	0.0
32	0.0	0.0	0.0	11.867675136249614	0.0
33	0.0	0.0	0.0	12.222904642972898	0.0
34	0.0	0.0	0.0	12.571393164232195	0.0
35	0.0	0.0	0.0	12.939628006547608	0.0
36	0.0	0.0	0.0	13.291657247444554	0.0
37	0.0	0.0	0.0	13.641099039375526	0.0
38	0.0	0.0	0.0	13.997417998295008	0.0
39	0.0	0.0	0.0	14.385194889379747	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTGC	45	3.8562575E-10	45.000004	16
GTCGCAC	45	3.8562575E-10	45.000004	13
TCGCACC	45	3.8562575E-10	45.000004	26
GGTCGAT	35	1.2122291E-7	45.000004	8
CGACGGT	720	0.0	45.000004	28
GTTTACG	90	0.0	45.000004	1
TAGGTGC	45	3.8562575E-10	45.000004	5
CGTTGAT	35	1.2122291E-7	45.000004	25
CTCCCGA	35	1.2122291E-7	45.000004	43
GCGCATC	45	3.8562575E-10	45.000004	21
ATCGTCC	65	0.0	45.000004	22
AGTGCGC	35	1.2122291E-7	45.000004	35
AGGTTCC	45	3.8562575E-10	45.000004	24
CGGATAG	45	3.8562575E-10	45.000004	26
TACGAGT	45	3.8562575E-10	45.000004	33
TCGTCCT	65	0.0	45.000004	23
TCGTCCA	35	1.2122291E-7	45.000004	21
TTGCGCG	65	0.0	45.000004	15
CGGTGAT	45	3.8562575E-10	45.000004	32
TCTCCCG	35	1.2122291E-7	45.000004	42
>>END_MODULE