FastQCFastQC Report
Thu 26 May 2016
SRR1547542_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547542_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2518483
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125790.4994673380761355No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44670.17736867789061905No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34340.13635192296314885No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT32660.1296812406516145No Hit
ATGCTGGGGAAACTTATGTCTTTTAGAAAGACAGTGCCTCTGTATGCTTTG30340.12046934603092416No Hit
GTATTTGGGATTGGTTCTTTTTTTTATAGAGTGTAAACCAAGTTTTATATT27700.10998684525565589No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC26080.10355440159810489No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA207.034888E-445.00000434
ACCGGTC207.034888E-445.00000412
ACGTTAC453.8562575E-1045.00000430
GACCGTA207.034888E-445.0000049
CGGATAC406.8212103E-945.00000432
TCGAATA453.8562575E-1045.0000049
GCGTACC207.034888E-445.00000414
TCGCGCG207.034888E-445.0000042
CTTACCG650.045.0000041
TGTCACG502.1827873E-1145.01
GTCGCGC253.892134E-545.022
ATTAGCG1100.045.01
GGTCGAC351.212702E-745.09
CATTGCG253.892134E-545.01
CCGGATA253.892134E-545.010
TCTACGC253.892134E-545.032
TTGCGTA551.8189894E-1245.014
AGTTCGG502.1827873E-1145.02
CGTCGGA351.212702E-745.017
TAATGCG1650.045.01