##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547542_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2518483
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.380514381077816	31.0	31.0	33.0	30.0	34.0
2	31.792617619416134	31.0	31.0	34.0	30.0	34.0
3	31.936497486780734	31.0	31.0	34.0	30.0	34.0
4	35.65988374747815	37.0	35.0	37.0	33.0	37.0
5	35.5597703061724	37.0	35.0	37.0	33.0	37.0
6	35.60832969688499	37.0	35.0	37.0	33.0	37.0
7	35.977182692914745	37.0	35.0	37.0	35.0	37.0
8	35.92725541526387	37.0	35.0	37.0	35.0	37.0
9	37.596183496176074	39.0	37.0	39.0	35.0	39.0
10	37.11831646272776	39.0	37.0	39.0	33.0	39.0
11	36.84789653136432	39.0	37.0	39.0	32.0	39.0
12	36.371030100262736	38.0	35.0	39.0	32.0	39.0
13	36.15297224559387	38.0	35.0	39.0	32.0	39.0
14	37.238020665614975	39.0	35.0	41.0	32.0	41.0
15	37.46886200939216	39.0	35.0	41.0	32.0	41.0
16	37.548465882040894	39.0	35.0	41.0	33.0	41.0
17	37.47097042147991	39.0	35.0	41.0	32.0	41.0
18	37.39864315145268	39.0	35.0	41.0	32.0	41.0
19	37.36966538983984	39.0	35.0	41.0	32.0	41.0
20	37.268770525749034	39.0	35.0	41.0	32.0	41.0
21	37.13060322424253	39.0	35.0	41.0	32.0	41.0
22	37.05484055282486	39.0	35.0	41.0	32.0	41.0
23	36.97701354347041	39.0	35.0	41.0	32.0	41.0
24	36.924614142720046	39.0	35.0	41.0	32.0	41.0
25	36.835595078465886	38.0	35.0	41.0	31.0	41.0
26	36.811748183331	38.0	35.0	41.0	31.0	41.0
27	36.77358354215613	38.0	35.0	41.0	31.0	41.0
28	36.731771864253204	38.0	35.0	41.0	31.0	41.0
29	36.71548110509382	38.0	35.0	41.0	31.0	41.0
30	36.659432285228846	38.0	35.0	41.0	31.0	41.0
31	36.529058564222986	38.0	35.0	41.0	31.0	41.0
32	36.378812165895106	38.0	35.0	41.0	30.0	41.0
33	36.26804310372554	38.0	35.0	41.0	30.0	41.0
34	36.21080944362142	38.0	35.0	41.0	30.0	41.0
35	36.0915106435104	38.0	35.0	41.0	30.0	41.0
36	36.01188453525396	38.0	35.0	41.0	30.0	41.0
37	35.9509819998785	38.0	35.0	41.0	30.0	41.0
38	35.84037732238018	38.0	35.0	40.0	29.0	41.0
39	35.80253986229012	38.0	35.0	40.0	29.0	41.0
40	35.73063308348716	38.0	35.0	40.0	29.0	41.0
41	35.69284605057886	38.0	35.0	40.0	29.0	41.0
42	35.65178363324271	38.0	35.0	40.0	29.0	41.0
43	35.57835093586099	38.0	35.0	40.0	28.0	41.0
44	35.45684564874966	38.0	34.0	40.0	28.0	41.0
45	35.39250652079049	38.0	34.0	40.0	28.0	41.0
46	35.3301928184546	38.0	34.0	40.0	27.0	41.0
47	35.24928975101281	37.0	34.0	40.0	27.0	41.0
48	35.14924063414365	37.0	34.0	40.0	27.0	41.0
49	35.09419678433406	37.0	34.0	40.0	27.0	41.0
50	34.99983164468452	37.0	34.0	40.0	27.0	41.0
51	34.55333587719274	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	7.0
12	9.0
13	17.0
14	26.0
15	74.0
16	126.0
17	383.0
18	765.0
19	1533.0
20	2784.0
21	4556.0
22	6897.0
23	10542.0
24	15680.0
25	22827.0
26	30320.0
27	35989.0
28	41109.0
29	48242.0
30	57729.0
31	71261.0
32	89393.0
33	116171.0
34	194216.0
35	296592.0
36	187947.0
37	232646.0
38	351302.0
39	699108.0
40	230.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.205227114894164	24.57082299145954	29.337819631897453	12.886130261748837
2	30.52242163238743	26.288960457545272	29.927460300506297	13.261157609560994
3	27.733719068185092	25.784807759274138	32.764128247043956	13.717344925496816
4	26.12949938514574	27.17691562738363	32.10694692003083	14.586638067439802
5	23.715466810774583	31.51230323968834	30.28803450331013	14.484195446226956
6	21.747774354641265	41.152431840913756	26.21923594481281	10.880557859632168
7	87.48159110067449	4.060499912050231	6.481878178252544	1.976030809022733
8	87.64172718259366	3.3640092071298477	6.401750577629469	2.592513032647034
9	83.07266715717358	6.09497860418355	7.6503196567139815	3.182034581928883
10	42.64241608936808	34.25641546915345	13.220974689922466	9.880193751555996
11	31.935613621374454	25.074658038192037	25.777025296577342	17.212703043856163
12	31.59159700502247	23.16334873016812	28.450618884463385	16.794435380346027
13	23.094458052724594	32.61121873762896	27.362106474413366	16.932216735233073
14	16.829654994693232	36.22430645749842	29.01353711738376	17.932501430424587
15	15.84267195768246	29.186101315752378	38.7903749995533	16.180851727011856
16	18.672748634793244	26.80331771149537	37.11936908051394	17.404564573197437
17	18.81148294429623	26.51679602363804	27.788037481293305	26.88368355077243
18	21.276379471292838	27.70814811932421	31.025264018061666	19.990208391321286
19	23.809610785540343	29.810921892266094	27.510330623633354	18.869136698560204
20	25.757172075412065	27.833541064204127	27.515770406232644	18.893516454151168
21	23.357989710472534	27.990302098525184	30.07631975280357	18.575388438198708
22	21.936578487923086	25.2509149356974	29.71042488672745	23.102081689652067
23	18.435105577444837	29.266308329260116	29.757556433773825	22.54102965952123
24	19.02105354691693	28.038704251726138	33.85172740892037	19.08851479243656
25	18.95506143976354	29.28167472244204	30.2951419564873	21.46812188130712
26	18.08703890397513	34.066976032794344	27.082851065502528	20.763133997727998
27	18.24649997637467	33.66594096525567	29.889778886734593	18.197780171635067
28	16.081148850319813	31.324491767464778	33.730781585581475	18.863577796633926
29	16.65677314478597	29.205557472494352	33.14106150408798	20.9966078786317
30	19.036618472310515	30.580829809055686	31.042020136725164	19.340531581908632
31	24.084736724448806	30.48259607072988	25.59012707252739	19.842540132293923
32	22.854789966817325	31.39516923481318	26.858708198546505	18.891332599822988
33	22.249544666372575	30.06980789626136	25.98520617371648	21.695441263649588
34	19.336640350560238	30.706738937685902	28.34063203920773	21.615988672546134
35	18.485890117185626	30.090097888292277	28.661857157661974	22.762154836860127
36	23.681398683254958	29.41449277203777	27.42940889416367	19.4746996505436
37	20.053937231261834	31.10904461137915	29.028387326815384	19.808630830543624
38	18.52607303682415	33.054183808268704	26.282607426772387	22.137135728134755
39	19.6662038219039	30.59722856973821	28.41988609809953	21.31668151025836
40	20.63909107188732	29.55271089779046	27.49714808477961	22.311049945542614
41	18.16327527324981	28.83056983112453	28.555165947119754	24.450988948505906
42	20.695752165093033	30.07334176962878	26.51127682815409	22.719629237124096
43	20.87784590962099	29.291085149274387	27.78668746225406	22.04438147885056
44	19.453853768320055	31.68728953103912	27.5359809853789	21.322875715261922
45	18.987819254686254	32.865181142775235	25.98008404265584	22.166915559882675
46	22.27884802081253	31.412441537226975	26.455409863794994	19.853300578165506
47	20.408952532139388	30.475965094860673	27.59268178502694	21.522400587973
48	20.206687914907505	28.915025433961635	29.689062820753602	21.189223830377255
49	20.761029556284477	27.783788891963933	28.746948063576365	22.70823348817522
50	19.283870488702924	29.224775390582348	29.725711867024714	21.765642253690018
51	18.070243078869304	30.52996585643024	27.429369187721335	23.970421876979117
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4204.0
1	4880.0
2	5556.0
3	13045.0
4	20534.0
5	14568.5
6	8603.0
7	9445.0
8	10287.0
9	11503.0
10	12719.0
11	13834.0
12	14949.0
13	15146.0
14	15343.0
15	14793.0
16	14243.0
17	13222.0
18	12201.0
19	12649.0
20	13097.0
21	13042.5
22	12988.0
23	14369.5
24	15751.0
25	19164.0
26	28348.0
27	34119.0
28	37280.0
29	40441.0
30	45595.0
31	50749.0
32	61977.0
33	73205.0
34	79727.0
35	86249.0
36	92574.0
37	98899.0
38	106765.0
39	114631.0
40	139467.5
41	164304.0
42	190363.0
43	216422.0
44	220818.5
45	225215.0
46	229779.5
47	234344.0
48	235485.5
49	236627.0
50	216892.5
51	197158.0
52	173490.0
53	149822.0
54	134032.0
55	118242.0
56	105442.5
57	92643.0
58	82071.5
59	71500.0
60	62261.5
61	53023.0
62	44102.0
63	35181.0
64	27517.5
65	19854.0
66	15000.0
67	10146.0
68	7507.0
69	4868.0
70	4029.5
71	3191.0
72	2631.5
73	2072.0
74	1629.5
75	1003.0
76	819.0
77	538.5
78	258.0
79	169.5
80	81.0
81	74.5
82	68.0
83	72.5
84	77.0
85	48.5
86	20.0
87	16.5
88	13.0
89	7.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2518483.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.291276068978302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.38523051289478	12.862155562530523
2	10.801078047700233	3.735288447307319
3	4.187101084576975	2.1720096234641684
4	2.1647449337550433	1.4972480907392243
5	1.3194584528772635	1.1407560185123882
6	0.864788837796471	0.8971981521425807
7	0.6104700630194784	0.7389064474061469
8	0.4766050129161257	0.6592887083353359
9	0.37248179547294996	0.5796617000572342
>10	2.645756492877417	9.40481331989853
>50	0.6761823397561739	8.55118223391442
>100	1.478388797466701	54.046340071538566
>500	0.012585999474732894	1.45125629160993
>1k	0.004894555351285014	1.7569430167379867
>5k	0.0	0.0
>10k+	2.3307406434690543E-4	0.5069523158056459
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12579	0.4994673380761355	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4467	0.17736867789061905	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3434	0.13635192296314885	No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	3266	0.1296812406516145	No Hit
ATGCTGGGGAAACTTATGTCTTTTAGAAAGACAGTGCCTCTGTATGCTTTG	3034	0.12046934603092416	No Hit
GTATTTGGGATTGGTTCTTTTTTTTATAGAGTGTAAACCAAGTTTTATATT	2770	0.10998684525565589	No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	2608	0.10355440159810489	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9706442330561694E-5	0.0	0.0	0.1421093571010803	0.0
2	3.9706442330561694E-5	0.0	0.0	0.44896074343166104	0.0
3	3.9706442330561694E-5	0.0	0.0	0.6587298782640184	0.0
4	3.9706442330561694E-5	0.0	0.0	0.9418765185232538	0.0
5	3.9706442330561694E-5	0.0	0.0	1.5166669777004649	0.0
6	3.9706442330561694E-5	0.0	0.0	2.0069621275982406	0.0
7	3.9706442330561694E-5	0.0	0.0	2.314012046140474	0.0
8	3.9706442330561694E-5	0.0	0.0	3.0429429144449256	0.0
9	3.9706442330561694E-5	0.0	0.0	3.251917920430672	0.0
10	3.9706442330561694E-5	0.0	0.0	3.7260922547422397	0.0
11	3.9706442330561694E-5	0.0	0.0	4.488058883065719	0.0
12	3.9706442330561694E-5	0.0	0.0	5.048832968100242	0.0
13	3.9706442330561694E-5	0.0	0.0	5.2715066966900315	0.0
14	3.9706442330561694E-5	0.0	0.0	5.35699466702773	0.0
15	3.9706442330561694E-5	0.0	0.0	5.50112905268767	0.0
16	3.9706442330561694E-5	0.0	0.0	5.851339874043224	0.0
17	3.9706442330561694E-5	0.0	0.0	6.2773105873654895	0.0
18	3.9706442330561694E-5	0.0	0.0	6.784441268811423	0.0
19	3.9706442330561694E-5	0.0	0.0	7.071320314649731	0.0
20	3.9706442330561694E-5	0.0	0.0	7.360700866354866	0.0
21	3.9706442330561694E-5	0.0	0.0	7.7259604293537025	0.0
22	3.9706442330561694E-5	0.0	0.0	8.11961009861889	0.0
23	3.9706442330561694E-5	0.0	0.0	8.527593793565412	0.0
24	3.9706442330561694E-5	0.0	0.0	8.830752480759251	0.0
25	3.9706442330561694E-5	0.0	0.0	9.095197386680791	0.0
26	3.9706442330561694E-5	0.0	0.0	9.339114061917432	0.0
27	3.9706442330561694E-5	0.0	0.0	9.586802849175475	0.0
28	3.9706442330561694E-5	0.0	0.0	9.861730255872285	0.0
29	3.9706442330561694E-5	0.0	0.0	10.160521234409762	0.0
30	3.9706442330561694E-5	0.0	0.0	10.510652642880654	0.0
31	3.9706442330561694E-5	0.0	0.0	10.836046937779608	0.0
32	3.9706442330561694E-5	0.0	0.0	11.117406788133968	0.0
33	3.9706442330561694E-5	0.0	0.0	11.415760995805808	0.0
34	3.9706442330561694E-5	0.0	0.0	11.722294730597746	0.0
35	3.9706442330561694E-5	0.0	0.0	12.066748117815367	0.0
36	3.9706442330561694E-5	0.0	0.0	12.381223141073416	0.0
37	3.9706442330561694E-5	0.0	0.0	12.707689509915294	0.0
38	3.9706442330561694E-5	0.0	0.0	13.027961673753605	0.0
39	3.9706442330561694E-5	0.0	0.0	13.370112087316055	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCGA	20	7.034888E-4	45.000004	34
ACCGGTC	20	7.034888E-4	45.000004	12
ACGTTAC	45	3.8562575E-10	45.000004	30
GACCGTA	20	7.034888E-4	45.000004	9
CGGATAC	40	6.8212103E-9	45.000004	32
TCGAATA	45	3.8562575E-10	45.000004	9
GCGTACC	20	7.034888E-4	45.000004	14
TCGCGCG	20	7.034888E-4	45.000004	2
CTTACCG	65	0.0	45.000004	1
TGTCACG	50	2.1827873E-11	45.0	1
GTCGCGC	25	3.892134E-5	45.0	22
ATTAGCG	110	0.0	45.0	1
GGTCGAC	35	1.212702E-7	45.0	9
CATTGCG	25	3.892134E-5	45.0	1
CCGGATA	25	3.892134E-5	45.0	10
TCTACGC	25	3.892134E-5	45.0	32
TTGCGTA	55	1.8189894E-12	45.0	14
AGTTCGG	50	2.1827873E-11	45.0	2
CGTCGGA	35	1.212702E-7	45.0	17
TAATGCG	165	0.0	45.0	1
>>END_MODULE