FastQCFastQC Report
Thu 26 May 2016
SRR1547541_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547541_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3310792
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213080.6435922280831898No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT36200.10933939673649085No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGA302.1667438E-645.00000443
CTCGTCG207.0352113E-445.030
TAGCACG700.045.01
GTATCGT453.8562575E-1045.045
ACGTCGA406.8212103E-945.036
CGACGGT7550.043.50993328
TCGTTAG2450.043.1632651
CGTTTTT103450.042.738041
ATAACGG2800.042.5892832
TATAGCG1750.042.428571
GTCGACA700.041.78571338
TCGATAG2550.041.470591
TTACGCG1600.040.781251
ACGGGAT14700.040.5612265
CCCGTAG501.0822987E-940.530
TCACGAC8550.040.26315725
CGTACGT451.9301297E-840.08
TACGGGA11250.039.84
TCTCACG8650.039.79768823
TAGGGAC31050.039.7101445
Produced by FastQC (version 0.11.5)