##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547541_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3310792
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.434005518921154	31.0	31.0	33.0	30.0	34.0
2	31.860589852820716	31.0	31.0	34.0	30.0	34.0
3	31.99355078784774	33.0	31.0	34.0	30.0	34.0
4	35.72877426307663	37.0	35.0	37.0	35.0	37.0
5	35.631293056162995	37.0	35.0	37.0	33.0	37.0
6	35.67855455733854	37.0	35.0	37.0	33.0	37.0
7	36.02439386104594	37.0	35.0	37.0	35.0	37.0
8	35.984321576227075	37.0	35.0	37.0	35.0	37.0
9	37.67775082215977	39.0	38.0	39.0	35.0	39.0
10	37.218333860900955	39.0	37.0	39.0	33.0	39.0
11	36.90351009667777	39.0	37.0	39.0	32.0	39.0
12	36.6073401772144	39.0	35.0	39.0	32.0	39.0
13	36.523942911545035	39.0	35.0	39.0	32.0	39.0
14	37.60116370946891	40.0	36.0	41.0	32.0	41.0
15	37.79754542115602	40.0	36.0	41.0	33.0	41.0
16	37.84808468789341	40.0	36.0	41.0	33.0	41.0
17	37.77453642512124	40.0	36.0	41.0	33.0	41.0
18	37.694494247902014	39.0	36.0	41.0	33.0	41.0
19	37.68038855959541	39.0	36.0	41.0	33.0	41.0
20	37.5794876271297	39.0	35.0	41.0	33.0	41.0
21	37.45878871279138	39.0	35.0	41.0	32.0	41.0
22	37.4117356209632	39.0	35.0	41.0	32.0	41.0
23	37.34172306807555	39.0	35.0	41.0	32.0	41.0
24	37.268868899042886	39.0	35.0	41.0	32.0	41.0
25	37.18052719711779	39.0	35.0	41.0	32.0	41.0
26	37.18615515562439	39.0	35.0	41.0	32.0	41.0
27	37.14919572114467	39.0	35.0	41.0	32.0	41.0
28	37.10016847932459	39.0	35.0	41.0	32.0	41.0
29	37.06610412251812	39.0	35.0	41.0	32.0	41.0
30	36.98392831684986	39.0	35.0	41.0	31.0	41.0
31	36.85604471679284	39.0	35.0	41.0	31.0	41.0
32	36.75607860596498	39.0	35.0	41.0	31.0	41.0
33	36.643155172538776	39.0	35.0	41.0	30.0	41.0
34	36.55330144569638	39.0	35.0	41.0	30.0	41.0
35	36.45019107210601	39.0	35.0	41.0	30.0	41.0
36	36.37375920927681	39.0	35.0	41.0	30.0	41.0
37	36.331340658066104	39.0	35.0	41.0	30.0	41.0
38	36.20264335542674	39.0	35.0	41.0	30.0	41.0
39	36.14131301513354	39.0	35.0	41.0	29.0	41.0
40	36.0670271644972	39.0	35.0	41.0	29.0	41.0
41	36.02655225698262	39.0	35.0	41.0	29.0	41.0
42	36.01273954993246	39.0	35.0	41.0	29.0	41.0
43	35.99372174392109	38.0	35.0	41.0	29.0	41.0
44	35.88377252331164	38.0	35.0	40.0	29.0	41.0
45	35.818946946833265	38.0	35.0	40.0	29.0	41.0
46	35.773506762128214	38.0	35.0	40.0	29.0	41.0
47	35.72035996220844	38.0	35.0	40.0	28.0	41.0
48	35.61521442603462	38.0	35.0	40.0	28.0	41.0
49	35.539164042923865	38.0	34.0	40.0	28.0	41.0
50	35.450236982570935	38.0	34.0	40.0	28.0	41.0
51	35.02909032038256	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	1.0
12	5.0
13	6.0
14	20.0
15	58.0
16	147.0
17	390.0
18	837.0
19	1766.0
20	3132.0
21	5047.0
22	8017.0
23	12241.0
24	18557.0
25	27677.0
26	37575.0
27	44737.0
28	50098.0
29	58113.0
30	70114.0
31	86175.0
32	108071.0
33	140132.0
34	224225.0
35	307432.0
36	244935.0
37	318339.0
38	508543.0
39	1034082.0
40	313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.27913200225203	24.783435504253966	30.509588038149182	13.427844455344824
2	30.44422603413322	26.26256798977405	30.166950989370516	13.126254986722211
3	28.397253587661197	25.819652820231532	32.607394242827695	13.175699349279569
4	26.27262600610368	28.13936967347994	31.337093964223666	14.250910356192717
5	23.6229578904383	31.90182288709167	30.189332341022933	14.2858868814471
6	21.90143627265017	40.62644225309231	26.72973717466999	10.742384299587531
7	88.83203777223093	3.3622166539003357	6.1560194660371295	1.6497261078316006
8	89.19376390905862	2.86568893485305	6.007233314566425	1.9333138415219078
9	84.78134536992961	5.485575656821691	7.16456968604491	2.5685092872037867
10	42.56625000906127	35.59577285435026	12.421408533063993	9.416568603524473
11	27.588383685837105	25.274496253464427	29.99067896744948	17.14644109324899
12	27.99940316395594	23.540802321619722	30.197638510664515	18.262156003759824
13	24.736649115981916	27.000004832680517	29.494996967492977	18.76834908384459
14	16.84137813550353	31.87451824216079	30.86285698406907	20.421246638266613
15	17.734668925139363	29.914594453532565	34.900380331956825	17.450356289371243
16	22.213597229907528	27.70720721809162	33.23038717019976	16.848808381801092
17	22.1947195716312	27.735780441658676	28.147343596335862	21.922156390374266
18	21.60700521204594	28.993757384939915	30.662361151047847	18.736876251966297
19	23.537328832496875	29.291118258108632	27.508191393479265	19.663361515915227
20	24.521111564846116	29.20914391480951	28.49263861939983	17.777105900944548
21	23.954117322984953	27.622907147292853	30.991043834828645	17.43193169489355
22	22.951034072813997	24.014676850735412	31.676317932385967	21.35797114406462
23	19.808462748490392	28.379342465488623	30.933112077110252	20.879082708910737
24	19.73908357879323	28.656526897491595	32.654392060872446	18.949997462842727
25	20.519229235784067	30.0930109774338	29.3347634040435	20.05299638273863
26	19.34654910365858	31.85573119664419	27.635894976186965	21.161824723510268
27	18.777440564070467	30.911062972243496	31.180635932429464	19.13086053125657
28	17.852012448985015	29.90822135609848	32.85950914463971	19.380257050276793
29	19.251919178251004	28.570988452309898	31.947823964779428	20.22926840465967
30	20.654514086055542	29.4509893705192	31.159251321134036	18.735245222291223
31	23.06907229448422	29.40284379085125	28.212192128046702	19.315891786617826
32	23.573543732134183	30.164353423591695	27.856265207841506	18.40583763643261
33	22.54149460310403	29.43322926961283	28.23445266268615	19.79082346459699
34	19.505000616166765	29.30446853804165	30.459811428806162	20.730719416985423
35	20.1986714961254	28.866778704310025	29.696036477072557	21.238513322492018
36	22.591331620953536	29.959598790863335	28.041205850443035	19.407863737740094
37	21.49186659868696	29.60083267085338	29.401363782442385	19.505936948017272
38	21.30348267121583	30.565949174698986	27.3929923716138	20.737575782471385
39	20.41121882619023	29.449509362110337	28.801356291787584	21.337915519911853
40	21.698373078103366	28.671659228365904	28.910121807712475	20.71984588581826
41	19.131917680120043	28.510579945825654	29.68042087814638	22.677081495907927
42	20.163513745351565	29.282026777882752	28.784894973770626	21.769564502995053
43	20.83262856742435	28.69298343115484	28.732430185889058	21.74195781553175
44	21.0656241769341	29.524929382455923	28.389007826526104	21.02043861408388
45	20.380410487883264	30.47234619390164	27.659424089462583	21.487819228752517
46	21.742259858064173	30.190178060113716	27.622182245215043	20.44537983660707
47	20.73848191006865	29.465547820581904	28.879917554470353	20.9160527148791
48	20.815714185608762	29.228414228377986	29.306492223008874	20.649379363004382
49	21.05025021203386	27.959261711397154	29.612159265819177	21.378328810749814
50	19.28200261448016	29.1294046862503	30.625572370598935	20.963020328670602
51	19.149556964013446	29.398373561371415	29.106328636773316	22.34574083784182
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2423.0
1	3805.5
2	5188.0
3	18749.5
4	32311.0
5	22245.0
6	12179.0
7	12119.5
8	12060.0
9	13950.0
10	15840.0
11	15772.5
12	15705.0
13	15560.0
14	15415.0
15	14881.0
16	14347.0
17	14244.5
18	14142.0
19	14046.5
20	13951.0
21	15128.0
22	16305.0
23	18445.0
24	20585.0
25	24984.5
26	36089.5
27	42795.0
28	51065.5
29	59336.0
30	72374.5
31	85413.0
32	92340.0
33	99267.0
34	115324.0
35	131381.0
36	143170.5
37	154960.0
38	163104.5
39	171249.0
40	193236.5
41	215224.0
42	236534.0
43	257844.0
44	261178.5
45	264513.0
46	273560.0
47	282607.0
48	285287.5
49	287968.0
50	268944.0
51	249920.0
52	229179.5
53	208439.0
54	183969.5
55	159500.0
56	145377.5
57	131255.0
58	118245.0
59	105235.0
60	90078.0
61	74921.0
62	60818.0
63	46715.0
64	37041.0
65	27367.0
66	21435.0
67	15503.0
68	12431.5
69	9360.0
70	7128.0
71	4896.0
72	3704.0
73	2512.0
74	1997.5
75	994.5
76	506.0
77	469.5
78	433.0
79	295.0
80	157.0
81	123.0
82	89.0
83	71.0
84	53.0
85	39.0
86	25.0
87	20.5
88	16.0
89	14.0
90	12.0
91	7.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3310792.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.05544096300158
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.20586805102583	12.826692426155576
2	10.03315183858652	3.422396577116863
3	3.707777446265	1.8971333801616443
4	1.9302030446798353	1.3168185630057132
5	1.1880881813283883	1.0131683917743124
6	0.7854470674581556	0.8037687653157176
7	0.5735055949372988	0.6846973571482938
8	0.450474628938757	0.6146434751356009
9	0.3527898624911402	0.5415288004856779
>10	3.0129927156462495	11.659877262406463
>50	1.099072398873595	13.736095672262227
>100	1.6500439412425933	49.07253760871314
>500	0.007714658078697363	0.8831257624316028
>1k	0.002691159794894429	0.8755065422089678
>5k	0.0	0.0
>10k+	1.7941065299296194E-4	0.6520094156783408
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21308	0.6435922280831898	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3620	0.10933939673649085	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0204253242124544E-5	0.0	0.0	0.08001106683838792	0.0
2	3.0204253242124544E-5	0.0	0.0	0.23933850269059487	0.0
3	3.0204253242124544E-5	0.0	0.0	0.35704447757515423	0.0
4	3.0204253242124544E-5	0.0	0.0	0.5198151982969634	0.0
5	3.0204253242124544E-5	0.0	0.0	0.8825682797348792	0.0
6	3.0204253242124544E-5	0.0	0.0	1.2599704239952254	0.0
7	3.0204253242124544E-5	0.0	0.0	1.508491019671426	0.0
8	3.0204253242124544E-5	0.0	0.0	2.0817677462069497	0.0
9	3.0204253242124544E-5	0.0	0.0	2.29374119546018	0.0
10	3.0204253242124544E-5	0.0	0.0	2.661628999949257	0.0
11	3.0204253242124544E-5	0.0	0.0	3.1778196878571654	0.0
12	3.0204253242124544E-5	0.0	0.0	3.5729215245173966	0.0
13	3.0204253242124544E-5	0.0	0.0	3.7437265766016106	0.0
14	3.0204253242124544E-5	0.0	0.0	3.8253988773683156	0.0
15	3.0204253242124544E-5	0.0	0.0	3.933983167773753	0.0
16	3.0204253242124544E-5	0.0	0.0	4.159790165011876	0.0
17	6.040850648424909E-5	0.0	0.0	4.43948155003395	0.0
18	6.040850648424909E-5	0.0	0.0	4.765989527581316	0.0
19	6.040850648424909E-5	0.0	0.0	4.981376057450906	0.0
20	9.061275972637363E-5	0.0	0.0	5.192564196119841	0.0
21	9.061275972637363E-5	0.0	0.0	5.459418773514011	0.0
22	9.061275972637363E-5	0.0	0.0	5.756507808403549	0.0
23	9.061275972637363E-5	0.0	0.0	6.058822179103973	0.0
24	9.061275972637363E-5	0.0	0.0	6.2950496437106285	0.0
25	9.061275972637363E-5	0.0	0.0	6.508261467346786	0.0
26	9.061275972637363E-5	0.0	0.0	6.712230789490853	0.0
27	9.061275972637363E-5	0.0	0.0	6.9342320508204685	0.0
28	9.061275972637363E-5	0.0	0.0	7.154753303741219	0.0
29	9.061275972637363E-5	0.0	0.0	7.407895150163466	0.0
30	9.061275972637363E-5	0.0	0.0	7.7006951810926205	0.0
31	9.061275972637363E-5	0.0	0.0	7.960481963228134	0.0
32	9.061275972637363E-5	0.0	0.0	8.197615555432053	0.0
33	9.061275972637363E-5	0.0	0.0	8.444535325686422	0.0
34	9.061275972637363E-5	0.0	0.0	8.713413588047814	0.0
35	1.2081701296849817E-4	0.0	0.0	9.013976112060195	0.0
36	1.2081701296849817E-4	0.0	0.0	9.282552331889168	0.0
37	1.2081701296849817E-4	0.0	0.0	9.561760448859367	0.0
38	1.2081701296849817E-4	0.0	0.0	9.885187592576036	0.0
39	1.2081701296849817E-4	0.0	0.0	10.328435008904213	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGA	30	2.1667438E-6	45.000004	43
CTCGTCG	20	7.0352113E-4	45.0	30
TAGCACG	70	0.0	45.0	1
GTATCGT	45	3.8562575E-10	45.0	45
ACGTCGA	40	6.8212103E-9	45.0	36
CGACGGT	755	0.0	43.509933	28
TCGTTAG	245	0.0	43.163265	1
CGTTTTT	10345	0.0	42.73804	1
ATAACGG	280	0.0	42.589283	2
TATAGCG	175	0.0	42.42857	1
GTCGACA	70	0.0	41.785713	38
TCGATAG	255	0.0	41.47059	1
TTACGCG	160	0.0	40.78125	1
ACGGGAT	1470	0.0	40.561226	5
CCCGTAG	50	1.0822987E-9	40.5	30
TCACGAC	855	0.0	40.263157	25
CGTACGT	45	1.9301297E-8	40.0	8
TACGGGA	1125	0.0	39.8	4
TCTCACG	865	0.0	39.797688	23
TAGGGAC	3105	0.0	39.710144	5
>>END_MODULE