FastQCFastQC Report
Thu 26 May 2016
SRR1547540_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547540_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2901554
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160250.5522902554975713No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT33860.11669608768266936No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG207.035067E-445.022
CGCGTAG207.035067E-445.014
CGGTCTA7100.043.73239531
ACGGTCT7450.041.97986630
CGACGGT7400.041.95945728
CTATCGG700.041.7857132
CGTTTTT77900.041.476251
CACGACG7850.040.70063826
TAGTACG1350.040.0000041
TCACGAC7950.039.62264325
GGGCGAT26000.039.3757
TATAGCG1150.039.1304321
CTAACCT8300.039.03614415
AACCCGC1100.038.86363621
TAGGGCG6100.038.3606575
AGGGCGA14600.038.2191776
TAGGGAC29750.038.1176455
TTAGGGA36850.037.917234
GTAGGGA42300.037.872344
TAGGGAT49500.037.55
Produced by FastQC (version 0.11.5)