##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547540_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2901554
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.359262312540107	31.0	31.0	33.0	30.0	34.0
2	31.775864588423996	31.0	31.0	34.0	30.0	34.0
3	31.929467795533014	31.0	31.0	34.0	30.0	34.0
4	35.66285169946863	37.0	35.0	37.0	33.0	37.0
5	35.55839767241967	37.0	35.0	37.0	33.0	37.0
6	35.59540163650237	37.0	35.0	37.0	33.0	37.0
7	35.932963853162825	37.0	35.0	37.0	35.0	37.0
8	35.87349020559328	37.0	35.0	37.0	35.0	37.0
9	37.496906485283404	39.0	37.0	39.0	35.0	39.0
10	37.09055251082696	39.0	37.0	39.0	33.0	39.0
11	36.87820526517859	39.0	37.0	39.0	32.0	39.0
12	36.525727248226296	39.0	35.0	39.0	32.0	39.0
13	36.38886093451992	39.0	35.0	39.0	32.0	39.0
14	37.422784824959315	40.0	36.0	41.0	32.0	41.0
15	37.622608092077556	40.0	36.0	41.0	32.0	41.0
16	37.68471653465694	40.0	36.0	41.0	33.0	41.0
17	37.614233614125396	40.0	36.0	41.0	32.0	41.0
18	37.52857882362348	39.0	36.0	41.0	32.0	41.0
19	37.517688107820845	39.0	36.0	41.0	32.0	41.0
20	37.41271539319965	39.0	35.0	41.0	32.0	41.0
21	37.2754761758699	39.0	35.0	41.0	32.0	41.0
22	37.23660734902745	39.0	35.0	41.0	32.0	41.0
23	37.14338730211466	39.0	35.0	41.0	32.0	41.0
24	37.078601328805185	39.0	35.0	41.0	31.0	41.0
25	36.99231032750037	39.0	35.0	41.0	31.0	41.0
26	36.99671968882881	39.0	35.0	41.0	31.0	41.0
27	36.97705402001824	39.0	35.0	41.0	31.0	41.0
28	36.92275656424109	39.0	35.0	41.0	31.0	41.0
29	36.879434951064155	39.0	35.0	41.0	31.0	41.0
30	36.78640170060595	39.0	35.0	41.0	31.0	41.0
31	36.665719473082355	39.0	35.0	41.0	30.0	41.0
32	36.56208121578988	39.0	35.0	41.0	30.0	41.0
33	36.47649742172643	39.0	35.0	41.0	30.0	41.0
34	36.38429028031186	39.0	35.0	41.0	30.0	41.0
35	36.2879119258163	39.0	35.0	41.0	30.0	41.0
36	36.22883427294477	39.0	35.0	41.0	30.0	41.0
37	36.19100075338939	39.0	35.0	41.0	30.0	41.0
38	36.05814091345534	39.0	35.0	41.0	29.0	41.0
39	35.984212942443946	38.0	35.0	41.0	29.0	41.0
40	35.895024872878466	38.0	35.0	41.0	29.0	41.0
41	35.82634374545502	38.0	35.0	41.0	28.0	41.0
42	35.807225024934915	38.0	35.0	40.0	28.0	41.0
43	35.78432832888859	38.0	35.0	40.0	28.0	41.0
44	35.67379514563575	38.0	35.0	40.0	28.0	41.0
45	35.615375071427245	38.0	34.0	40.0	28.0	41.0
46	35.58720878536123	38.0	34.0	40.0	28.0	41.0
47	35.510146631770425	38.0	34.0	40.0	28.0	41.0
48	35.416620197314955	38.0	34.0	40.0	27.0	41.0
49	35.35675675861969	38.0	34.0	40.0	27.0	41.0
50	35.26569589950764	38.0	34.0	40.0	27.0	41.0
51	34.84419590329872	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	11.0
11	4.0
12	4.0
13	24.0
14	28.0
15	72.0
16	220.0
17	454.0
18	1101.0
19	2023.0
20	3372.0
21	5582.0
22	8314.0
23	12333.0
24	17852.0
25	25903.0
26	34471.0
27	42191.0
28	47079.0
29	55103.0
30	66162.0
31	80578.0
32	99422.0
33	128099.0
34	201874.0
35	265346.0
36	218461.0
37	284520.0
38	437183.0
39	863442.0
40	323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.57447285144443	24.13065550391273	28.225495717122616	13.069375927520218
2	31.567325646877503	25.192707080412774	29.105920482610355	14.134046790099376
3	30.410876378657782	25.05619402568417	30.45264709876156	14.08028249689649
4	27.744132971504236	26.773618550611154	29.8383555846281	15.643892893256512
5	24.253520699597527	31.191113451619373	28.51730486491032	16.03806098387278
6	22.765042456559485	39.888004841543534	26.135787926056175	11.211164775840809
7	86.15173110684826	4.1570482575888645	7.2635215474190735	2.4276990881438016
8	85.94256732771474	3.7221778398747705	7.154959032297866	3.180295800112629
9	79.74850717925635	6.920395071055027	9.28454200748978	4.046555742198835
10	38.17992013934602	35.65341193029666	14.835739744978035	11.330928185379282
11	30.045796149235894	23.89560904260269	28.605774698661474	17.452820109499946
12	29.347446230537155	22.42953258839918	30.15966616509636	18.063355015967307
13	25.341834065469744	25.793109485468825	30.52995050238596	18.33510594667547
14	18.765254756589055	29.97128435314318	31.60778672394172	19.655674166326044
15	17.706442823397392	28.909094919481078	35.62832192680198	17.756140330319546
16	22.76735156402397	26.729676580204952	33.32035178390614	17.18262007186494
17	23.168378048452656	26.101220242669964	28.425560923560273	22.304840785317108
18	22.761044598859783	26.834206773335943	31.011795748071552	19.39295287973272
19	24.287019989977786	28.592264696779722	27.762295652605467	19.358419660637026
20	25.164722076514863	28.059619086875514	28.427180745214457	18.34847809139516
21	24.451483584313785	26.584340667104595	31.193491487664886	17.770684260916735
22	23.374474505730376	23.740416342415134	31.292645251475587	21.5924639003789
23	20.484781603237437	27.18384700060726	31.153754160701475	21.17761723545383
24	20.450524098465856	27.175368785140652	32.40680683523381	19.967300281159684
25	21.490656386198566	27.643290457458313	29.64845734389227	21.217595812450845
26	19.80997079496022	30.614698192761537	27.798241907612265	21.777089104665983
27	19.364381982896063	29.241813180109695	31.55478064513016	19.839024191864084
28	18.164748958661463	28.11207373703884	32.82158457157785	20.901592732721845
29	19.71026560250128	26.898999639503522	32.447578090912664	20.943156667082537
30	21.12647222833006	27.15855021136949	31.49274492220376	20.22223263809669
31	23.19998180285461	26.462716185878328	29.264869790464004	21.07243222080306
32	23.03679338726765	27.827777804583338	28.998012789008925	20.13741601914009
33	22.40168544166333	27.023967156909713	29.573945547799557	21.0004018536274
34	19.27601554201645	27.21669146946774	31.388524907687398	22.11876808082841
35	19.824445796976377	26.808978912679205	30.796945361003104	22.56962992934131
36	22.18866166199216	27.96098228742253	29.30860497512712	20.541751075458187
37	20.014137251969117	29.171781741783885	29.963943459263554	20.850137546983444
38	20.432292488783595	29.185774243732844	28.44503324770106	21.936900019782502
39	19.90995170174327	27.83939227048678	29.31518765461542	22.935468373154524
40	21.85422018683781	27.14945163867362	29.070456727670756	21.92587144681781
41	19.034662115542222	25.6845125060571	29.996374356637855	25.28445102176282
42	20.07475993898442	27.408175067567242	29.64718216514323	22.86988282830511
43	20.335413368146863	26.395820997989354	30.379686195742007	22.88907943812178
44	20.245323712741516	27.632985634594426	29.75281521557069	22.368875437093365
45	19.59501701502023	28.343811626459477	28.70758221284181	23.353589145678487
46	21.153595624965106	27.683303498745843	29.291028186964642	21.87207268932441
47	19.944002420771767	27.15599985387141	31.01693092735824	21.88306679799859
48	19.587400406816485	27.2849652289773	30.970025027967772	22.157609336238444
49	20.75453360509575	25.936480934009843	31.062320397966054	22.246665062928347
50	19.19385267342948	27.152863603434575	31.442702772376457	22.21058095075949
51	19.0207385421743	28.050451585598612	28.989810287866437	23.93899958436066
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1927.0
1	3473.0
2	5019.0
3	15425.5
4	25832.0
5	18025.0
6	10218.0
7	10032.0
8	9846.0
9	10512.5
10	11179.0
11	11372.0
12	11565.0
13	11372.0
14	11179.0
15	11300.5
16	11422.0
17	10701.0
18	9980.0
19	10656.5
20	11333.0
21	12499.0
22	13665.0
23	15058.0
24	16451.0
25	20719.0
26	30104.5
27	35222.0
28	41950.0
29	48678.0
30	58891.0
31	69104.0
32	74657.5
33	80211.0
34	96508.5
35	112806.0
36	117800.0
37	122794.0
38	131173.0
39	139552.0
40	158367.5
41	177183.0
42	193512.0
43	209841.0
44	226690.0
45	243539.0
46	251192.5
47	258846.0
48	253042.0
49	247238.0
50	224289.0
51	201340.0
52	183119.5
53	164899.0
54	151764.5
55	138630.0
56	132242.0
57	125854.0
58	115386.5
59	104919.0
60	96221.0
61	87523.0
62	74538.5
63	61554.0
64	49493.5
65	37433.0
66	30450.0
67	23467.0
68	19691.5
69	15916.0
70	12606.0
71	9296.0
72	7107.0
73	4918.0
74	3752.5
75	2183.0
76	1779.0
77	1418.0
78	1057.0
79	696.5
80	336.0
81	293.0
82	250.0
83	161.5
84	73.0
85	64.0
86	55.0
87	38.0
88	21.0
89	16.0
90	11.0
91	10.0
92	9.0
93	7.5
94	6.0
95	3.5
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2901554.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.514584918079887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.20219757762268	14.108520579951966
2	9.493377423105503	3.5153188491893848
3	3.4165559416273923	1.8976834532589224
4	1.7013138167271717	1.259964765283913
5	1.0739385110758612	0.9941762880055157
6	0.6882935493601846	0.7646081620917449
7	0.511504796661145	0.6629209295651569
8	0.40182839014990246	0.5951748681540564
9	0.30466133616381474	0.5076610361694568
>10	3.249518656133213	14.42886375637239
>50	1.5428914489112477	20.86710574709938
>100	1.4040839289888627	38.08500988550001
>500	0.007186840230252957	0.8999850449301935
>1k	0.0024586558682444326	0.8518728570952356
>5k	0.0	0.0
>10k+	1.8912737448034098E-4	0.5611337773327503
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16025	0.5522902554975713	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	3386	0.11669608768266936	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.892858102933807E-5	0.0	0.0	0.12786251780942212	0.0
2	6.892858102933807E-5	0.0	0.0	0.3666655867855639	0.0
3	6.892858102933807E-5	0.0	0.0	0.5349202530781781	0.0
4	6.892858102933807E-5	0.0	0.0	0.765279570878226	0.0
5	6.892858102933807E-5	0.0	0.0	1.2797624996812054	0.0
6	6.892858102933807E-5	0.0	0.0	1.7962098930435209	0.0
7	6.892858102933807E-5	0.0	0.0	2.118933509422882	0.0
8	6.892858102933807E-5	0.0	0.0	2.8871080807043397	0.0
9	6.892858102933807E-5	0.0	0.0	3.1514147246613367	0.0
10	6.892858102933807E-5	0.0	0.0	3.614166753401798	0.0
11	6.892858102933807E-5	0.0	0.0	4.206642371639473	0.0
12	6.892858102933807E-5	0.0	0.0	4.702411190692987	0.0
13	6.892858102933807E-5	0.0	0.0	4.906439790539828	0.0
14	6.892858102933807E-5	0.0	0.0	5.005421232897957	0.0
15	6.892858102933807E-5	0.0	0.0	5.146104466778836	0.0
16	6.892858102933807E-5	0.0	0.0	5.422301290963394	0.0
17	6.892858102933807E-5	0.0	0.0	5.738993656502688	0.0
18	6.892858102933807E-5	0.0	0.0	6.110691029703394	0.0
19	6.892858102933807E-5	0.0	0.0	6.347529634120199	0.0
20	6.892858102933807E-5	0.0	0.0	6.589124310628029	0.0
21	6.892858102933807E-5	0.0	0.0	6.895442924722407	0.0
22	6.892858102933807E-5	0.0	0.0	7.21692582664324	0.0
23	6.892858102933807E-5	0.0	0.0	7.533893906506651	0.0
24	6.892858102933807E-5	0.0	0.0	7.782484833988959	0.0
25	6.892858102933807E-5	0.0	0.0	8.002918436120781	0.0
26	6.892858102933807E-5	0.0	0.0	8.222455966699224	0.0
27	6.892858102933807E-5	0.0	0.0	8.451815820074346	0.0
28	6.892858102933807E-5	0.0	0.0	8.688068531552403	0.0
29	6.892858102933807E-5	0.0	0.0	8.948101603485581	0.0
30	6.892858102933807E-5	0.0	0.0	9.249836466941508	0.0
31	6.892858102933807E-5	0.0	0.0	9.516314361200928	0.0
32	6.892858102933807E-5	0.0	0.0	9.765422253040956	0.0
33	6.892858102933807E-5	0.0	0.0	10.008567822621947	0.0
34	6.892858102933807E-5	0.0	0.0	10.277906252994086	0.0
35	6.892858102933807E-5	0.0	0.0	10.559996470856651	0.0
36	6.892858102933807E-5	0.0	0.0	10.82106347150527	0.0
37	6.892858102933807E-5	0.0	0.0	11.099879581768942	0.0
38	6.892858102933807E-5	0.0	0.0	11.411609089474123	0.0
39	6.892858102933807E-5	0.0	0.0	11.881977726418326	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAACG	20	7.035067E-4	45.0	22
CGCGTAG	20	7.035067E-4	45.0	14
CGGTCTA	710	0.0	43.732395	31
ACGGTCT	745	0.0	41.979866	30
CGACGGT	740	0.0	41.959457	28
CTATCGG	70	0.0	41.785713	2
CGTTTTT	7790	0.0	41.47625	1
CACGACG	785	0.0	40.700638	26
TAGTACG	135	0.0	40.000004	1
TCACGAC	795	0.0	39.622643	25
GGGCGAT	2600	0.0	39.375	7
TATAGCG	115	0.0	39.130432	1
CTAACCT	830	0.0	39.036144	15
AACCCGC	110	0.0	38.863636	21
TAGGGCG	610	0.0	38.360657	5
AGGGCGA	1460	0.0	38.219177	6
TAGGGAC	2975	0.0	38.117645	5
TTAGGGA	3685	0.0	37.91723	4
GTAGGGA	4230	0.0	37.87234	4
TAGGGAT	4950	0.0	37.5	5
>>END_MODULE