FastQCFastQC Report
Thu 26 May 2016
SRR1547539_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547539_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3657123
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT648341.772814313327717No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT46230.12641084262137203No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAG453.8562575E-1045.00000419
GTACGAT453.8562575E-1045.00000431
CGTTAGT207.035309E-445.022
CGTCGAG207.035309E-445.021
AATCGCG207.035309E-445.01
CGGACCG253.8924816E-545.06
GCTAGCG1150.044.9999961
CGTTTTT260000.044.0913471
CCAGTCG1850.043.78378328
CGACGGT9050.042.76243228
CGGTCTA9200.042.06521631
TACGGGA19800.041.818184
TAGTACG700.041.7857131
TCACGAC9350.041.6310225
CTACGGG8300.041.204823
TATAGCG3400.041.029411
GACGGTC9400.040.9308529
ACGGGAT24650.040.527385
CGTAAGG4150.040.1204832
TCGCGAG900.040.0000041
Produced by FastQC (version 0.11.5)