##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547539_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3657123
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.356404747666403	31.0	31.0	33.0	30.0	34.0
2	31.760742802470684	31.0	31.0	34.0	30.0	34.0
3	31.77125789862687	31.0	31.0	34.0	30.0	34.0
4	35.594755494961476	37.0	35.0	37.0	33.0	37.0
5	35.52259275939037	37.0	35.0	37.0	33.0	37.0
6	35.57588328311626	37.0	35.0	37.0	33.0	37.0
7	35.989632560895544	37.0	35.0	37.0	35.0	37.0
8	35.95646441205286	37.0	35.0	37.0	35.0	37.0
9	37.6145437274054	39.0	37.0	39.0	35.0	39.0
10	37.12456540291371	39.0	37.0	39.0	33.0	39.0
11	36.85743246809035	39.0	37.0	39.0	32.0	39.0
12	36.62672160602747	39.0	35.0	39.0	32.0	39.0
13	36.55000693167826	39.0	35.0	39.0	32.0	39.0
14	37.62483186920429	40.0	36.0	41.0	32.0	41.0
15	37.80202525318399	40.0	36.0	41.0	33.0	41.0
16	37.8557940216941	40.0	36.0	41.0	33.0	41.0
17	37.774804402258276	40.0	36.0	41.0	33.0	41.0
18	37.64122699728721	39.0	36.0	41.0	33.0	41.0
19	37.568411836298644	39.0	36.0	41.0	33.0	41.0
20	37.390415088581925	39.0	35.0	41.0	32.0	41.0
21	37.264871320981	39.0	35.0	41.0	32.0	41.0
22	37.22502196398644	39.0	35.0	41.0	32.0	41.0
23	37.1378020372845	39.0	35.0	41.0	32.0	41.0
24	37.06242803427722	39.0	35.0	41.0	32.0	41.0
25	36.96999882147798	39.0	35.0	41.0	31.0	41.0
26	36.96013286947144	39.0	35.0	41.0	32.0	41.0
27	36.91418801063021	39.0	35.0	41.0	31.0	41.0
28	36.83601344554175	39.0	35.0	41.0	31.0	41.0
29	36.777551370298454	39.0	35.0	41.0	31.0	41.0
30	36.65290803727411	38.0	35.0	41.0	31.0	41.0
31	36.46969626124142	38.0	35.0	41.0	30.0	41.0
32	36.24703052098603	38.0	35.0	41.0	30.0	41.0
33	36.02921804926988	38.0	35.0	41.0	29.0	41.0
34	35.83092201164686	38.0	35.0	41.0	28.0	41.0
35	35.65279866168024	38.0	35.0	41.0	27.0	41.0
36	35.56343798116716	38.0	35.0	41.0	26.0	41.0
37	35.50293468390316	38.0	35.0	41.0	26.0	41.0
38	35.346895633534885	38.0	35.0	41.0	25.0	41.0
39	35.28478971038163	38.0	35.0	41.0	25.0	41.0
40	35.193169330099096	38.0	34.0	40.0	24.0	41.0
41	35.10510830508025	38.0	34.0	40.0	24.0	41.0
42	35.07577185673	38.0	34.0	40.0	24.0	41.0
43	35.04827510586874	38.0	34.0	40.0	24.0	41.0
44	34.93825009440481	38.0	34.0	40.0	24.0	41.0
45	34.86169674905657	38.0	34.0	40.0	23.0	41.0
46	34.83531398861892	38.0	34.0	40.0	23.0	41.0
47	34.775169443302836	38.0	34.0	40.0	23.0	41.0
48	34.67673633071679	38.0	34.0	40.0	23.0	41.0
49	34.59663347390831	38.0	34.0	40.0	23.0	41.0
50	34.50954042289526	37.0	34.0	40.0	23.0	41.0
51	34.114316362889625	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	6.0
11	6.0
12	8.0
13	9.0
14	47.0
15	92.0
16	257.0
17	674.0
18	1420.0
19	2862.0
20	5313.0
21	8348.0
22	12888.0
23	18957.0
24	29341.0
25	45316.0
26	61908.0
27	69489.0
28	71834.0
29	76267.0
30	86151.0
31	103012.0
32	124774.0
33	157894.0
34	237240.0
35	318839.0
36	289446.0
37	357066.0
38	526053.0
39	1051273.0
40	328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.615896703501633	23.75766415294208	29.390589269215173	16.235849874341117
2	34.0503723828813	23.984263039553223	29.327944397823096	12.637420179742382
3	27.079373595036316	24.379628467513946	35.34759427014077	13.193403667308976
4	24.88693981580603	26.222552536515725	34.901943412895875	13.98856423478237
5	22.6867403694106	30.42090736352045	33.00102293524172	13.891329331827231
6	21.17341965255202	39.64843402860664	28.87337942967737	10.304766889163968
7	85.28884590428049	2.7051318755207303	10.154375447585439	1.8516467726133357
8	85.89142339483796	2.1855157729176735	9.936089106108819	1.9869717261355442
9	80.69611002965993	5.504818951946653	11.346760828115434	2.4523101902779865
10	35.33952782009246	40.11631547530668	16.146572045840404	8.39758465876045
11	24.184119593461855	24.080240123178793	34.78567715660644	16.949963126752916
12	24.944772161067593	22.696420109468562	34.54387506244663	17.814932667017217
13	22.24431609218503	25.32233671112511	35.13507749124107	17.298269705448792
14	16.61262145134304	30.240875136001716	34.89092382181294	18.25557959084231
15	15.347118486307407	30.725764487549366	37.76536911665262	16.161747909490604
16	20.82773808810915	28.76646478666427	35.65359983790537	14.75219728732121
17	20.37377468572974	28.01497242504559	31.962255576309573	19.6489973129151
18	19.758536970181208	29.280119919401127	34.04941534643489	16.91192776398278
19	21.153376574974374	30.227039123376493	31.23818914485512	17.381395156794017
20	22.165538320696353	30.434223842074772	31.203735832784407	16.196502004444476
21	22.28596084955305	28.084261863765587	33.83159385123224	15.798183435449124
22	20.73386648466568	24.51366825780812	34.44303076489361	20.309434492632597
23	17.12272187727894	29.118490135551905	34.62834036481682	19.130447622352325
24	17.624865228760424	29.57239885013438	35.153015088636614	17.64972083246858
25	18.688296784111444	30.719803517683165	32.1911513503921	18.400748347813295
26	18.23187243086984	32.39248447481805	30.03615136816563	19.33949172614648
27	16.953435801858454	31.40001033599362	33.65826087883836	17.98829298330956
28	15.89610193586598	30.07727659146274	35.16439015039964	18.862231322271633
29	17.161030679033765	29.145095748762074	34.089501501590185	19.604372070613977
30	18.74768773158573	28.69230813401682	33.57546355427476	18.98454058012268
31	20.313426701809046	29.391163491083017	31.1884779374388	19.106931869669136
32	20.102386493426664	30.157612965164148	30.600720839851437	19.139279701557754
33	19.80906849455159	29.80873216460043	31.311443448853097	19.07075589199488
34	17.485848848944922	29.457609164362257	31.970021243474722	21.0865207432181
35	17.782858274113288	29.85086911214088	30.943339887665793	21.422932726080035
36	20.46395486287992	29.871021565312404	30.352000739379015	19.31302283242866
37	18.81063338586096	31.458143464138338	29.97758073764541	19.753642412355283
38	18.388498281299263	31.466045850795833	29.697360466136907	20.448095401768
39	18.76887925289907	29.12590033203696	30.415137800943526	21.690082614120442
40	20.121663941847185	28.844367553401952	30.81632747927811	20.217641025472755
41	18.384205289239656	28.817351781714752	30.089499314078306	22.708943614967282
42	19.230745041935972	30.098495456674552	29.47964834652813	21.191111154861346
43	19.02894707123605	29.80282588253116	30.389297816890494	20.7789292293423
44	19.597454064301363	30.242789208894532	30.18353498091259	19.97622174589151
45	18.845715607596464	30.820210312860684	29.06352889962957	21.27054517991328
46	19.98087020863121	30.44948173742037	29.27249097172832	20.297157082220092
47	18.994985949337774	29.88324428792797	30.081542239623882	21.04022752311038
48	18.25276316930002	30.318805246637865	30.71086206288386	20.71756952117826
49	19.268753060807633	28.810105648620514	30.87046839824638	21.05067289232547
50	18.115469455088057	29.657629781661704	31.44786215831406	20.779038604936176
51	17.32807455477981	29.901756107191364	30.55204870057693	22.2181206374519
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2986.0
1	4140.5
2	5295.0
3	45918.0
4	86541.0
5	56917.5
6	27294.0
7	26771.5
8	26249.0
9	29298.5
10	32348.0
11	35101.0
12	37854.0
13	40382.5
14	42911.0
15	40850.0
16	38789.0
17	36921.0
18	35053.0
19	32974.5
20	30896.0
21	30170.0
22	29444.0
23	31329.0
24	33214.0
25	38323.5
26	48503.0
27	53573.0
28	62731.0
29	71889.0
30	84428.0
31	96967.0
32	107892.0
33	118817.0
34	131893.5
35	144970.0
36	151553.5
37	158137.0
38	177191.0
39	196245.0
40	208154.0
41	220063.0
42	233421.5
43	246780.0
44	252780.0
45	258780.0
46	265466.0
47	272152.0
48	287329.0
49	302506.0
50	294978.0
51	287450.0
52	259480.0
53	231510.0
54	199622.5
55	167735.0
56	142929.0
57	118123.0
58	105946.0
59	93769.0
60	76601.0
61	59433.0
62	49396.5
63	39360.0
64	31105.5
65	22851.0
66	17256.5
67	11662.0
68	8714.5
69	5767.0
70	4289.5
71	2812.0
72	2160.5
73	1509.0
74	1294.0
75	758.5
76	438.0
77	325.5
78	213.0
79	171.0
80	129.0
81	81.5
82	34.0
83	39.0
84	44.0
85	26.5
86	9.0
87	7.5
88	6.0
89	4.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3657123.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.654252698425694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.87540565001196	15.464955491924778
2	9.122211842387129	3.768249371224704
3	3.7834618186920834	2.3443372943433465
4	2.1147834351664794	1.7471708588949284
5	1.4518279996178	1.4993211189377964
6	1.0172721704363552	1.2606597882761068
7	0.731093447072075	1.0570132161392825
8	0.5935531357232241	0.9807517164136347
9	0.47469751712302943	0.8824070226576877
>10	3.8628267951630804	17.653858523276927
>50	1.0451535298498866	15.468391383368244
>100	0.9181794260568245	33.74349300248367
>500	0.00577364797319107	0.8363057178700318
>1k	0.0036253138436316014	1.4950670264747783
>5k	0.0	0.0
>10k+	1.342708830974667E-4	1.798018467714075
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64834	1.772814313327717	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	4623	0.12641084262137203	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7343898468823716E-5	0.0	0.0	0.10068023416220893	0.0
2	2.7343898468823716E-5	0.0	0.0	0.287110933922649	0.0
3	2.7343898468823716E-5	0.0	0.0	0.4479204008178013	0.0
4	2.7343898468823716E-5	0.0	0.0	0.6292651354630402	0.0
5	2.7343898468823716E-5	0.0	0.0	1.090912173312191	0.0
6	2.7343898468823716E-5	0.0	0.0	1.7134780536503695	0.0
7	2.7343898468823716E-5	0.0	0.0	2.2012658584357156	0.0
8	2.7343898468823716E-5	0.0	0.0	3.056227531860427	0.0
9	2.7343898468823716E-5	0.0	0.0	3.4702961863738246	0.0
10	2.7343898468823716E-5	0.0	0.0	3.9980881146190597	0.0
11	2.7343898468823716E-5	0.0	0.0	4.619614926815423	0.0
12	2.7343898468823716E-5	0.0	0.0	5.08902763182972	0.0
13	2.7343898468823716E-5	0.0	0.0	5.29613031883259	0.0
14	2.7343898468823716E-5	0.0	0.0	5.405970758981855	0.0
15	2.7343898468823716E-5	0.0	0.0	5.529428460568594	0.0
16	2.7343898468823716E-5	0.0	0.0	5.753648428012949	0.0
17	2.7343898468823716E-5	0.0	0.0	6.060064154254587	0.0
18	2.7343898468823716E-5	0.0	0.0	6.412034815345287	0.0
19	2.7343898468823716E-5	0.0	0.0	6.6435556036808165	0.0
20	2.7343898468823716E-5	0.0	0.0	6.883060810369244	0.0
21	2.7343898468823716E-5	0.0	0.0	7.142773158026132	0.0
22	2.7343898468823716E-5	0.0	0.0	7.452798278865655	0.0
23	2.7343898468823716E-5	0.0	0.0	7.76230386563427	0.0
24	2.7343898468823716E-5	0.0	0.0	8.001371569947196	0.0
25	2.7343898468823716E-5	0.0	0.0	8.212903968502017	0.0
26	2.7343898468823716E-5	0.0	0.0	8.410955825111706	0.0
27	2.7343898468823716E-5	0.0	0.0	8.633097656272431	0.0
28	2.7343898468823716E-5	0.0	0.0	8.865903607835996	0.0
29	2.7343898468823716E-5	0.0	0.0	9.125780018883697	0.0
30	2.7343898468823716E-5	0.0	0.0	9.413137047892565	0.0
31	2.7343898468823716E-5	0.0	0.0	9.673204866229547	0.0
32	2.7343898468823716E-5	0.0	0.0	9.911807724268503	0.0
33	2.7343898468823716E-5	0.0	0.0	10.153773881819124	0.0
34	2.7343898468823716E-5	0.0	0.0	10.41490811219639	0.0
35	2.7343898468823716E-5	0.0	0.0	10.697534646770153	0.0
36	2.7343898468823716E-5	0.0	0.0	10.965012661592185	0.0
37	5.468779693764743E-5	0.0	0.0	11.232408644718813	0.0
38	5.468779693764743E-5	0.0	0.0	11.546726757617941	0.0
39	5.468779693764743E-5	0.0	0.0	12.01633086992152	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTAG	45	3.8562575E-10	45.000004	19
GTACGAT	45	3.8562575E-10	45.000004	31
CGTTAGT	20	7.035309E-4	45.0	22
CGTCGAG	20	7.035309E-4	45.0	21
AATCGCG	20	7.035309E-4	45.0	1
CGGACCG	25	3.8924816E-5	45.0	6
GCTAGCG	115	0.0	44.999996	1
CGTTTTT	26000	0.0	44.091347	1
CCAGTCG	185	0.0	43.783783	28
CGACGGT	905	0.0	42.762432	28
CGGTCTA	920	0.0	42.065216	31
TACGGGA	1980	0.0	41.81818	4
TAGTACG	70	0.0	41.785713	1
TCACGAC	935	0.0	41.63102	25
CTACGGG	830	0.0	41.20482	3
TATAGCG	340	0.0	41.02941	1
GACGGTC	940	0.0	40.93085	29
ACGGGAT	2465	0.0	40.52738	5
CGTAAGG	415	0.0	40.120483	2
TCGCGAG	90	0.0	40.000004	1
>>END_MODULE