SRR1547538_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1547538_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3044423
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16833	0.5529126537278164	No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	4214	0.13841703337545405	TruSeq Adapter, Index 14 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3330	0.10938033249650261	No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	3282	0.1078036790551116	TruSeq Adapter, Index 15 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATC	30	2.1666856E-6	45.000004	31
TCGCTAG	60	0.0	45.000004	1
CGTAAAC	30	2.1666856E-6	45.000004	15
CGTCGCA	55	1.8189894E-12	45.000004	31
CGCCGGT	60	0.0	45.000004	28
AGTCCGC	20	7.035121E-4	45.0	34
GTCGAGT	20	7.035121E-4	45.0	2
CGAAACA	20	7.035121E-4	45.0	44
ACGTTAC	25	3.892327E-5	45.0	45
CGTGACG	25	3.892327E-5	45.0	11
CGCGACA	25	3.892327E-5	45.0	19
CCCGTAC	25	3.892327E-5	45.0	27
GTCACGA	20	7.035121E-4	45.0	45
TCGTCAA	25	3.892327E-5	45.0	21
CCCGGTA	20	7.035121E-4	45.0	29
CAATCGT	25	3.892327E-5	45.0	40
ATCGCAA	40	6.8212103E-9	45.0	29
TAGTCGT	20	7.035121E-4	45.0	28
AATGTCG	20	7.035121E-4	45.0	15
AGTCGTG	40	6.8212103E-9	45.0	1