##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547538_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3044423
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.344740202002153	31.0	31.0	33.0	30.0	34.0
2	31.7486781567476	31.0	31.0	34.0	30.0	34.0
3	31.900673460948102	31.0	31.0	34.0	30.0	34.0
4	35.615253530800416	37.0	35.0	37.0	33.0	37.0
5	35.513878984622046	37.0	35.0	37.0	33.0	37.0
6	35.55677052761722	37.0	35.0	37.0	33.0	37.0
7	35.9737372237695	37.0	35.0	37.0	35.0	37.0
8	35.930719876968475	37.0	35.0	37.0	35.0	37.0
9	37.612455627880884	39.0	37.0	39.0	35.0	39.0
10	37.09839171494894	39.0	37.0	39.0	33.0	39.0
11	36.737206689083614	39.0	35.0	39.0	32.0	39.0
12	36.34563429589121	38.0	35.0	39.0	32.0	39.0
13	36.19317322198656	39.0	35.0	39.0	32.0	39.0
14	37.28915922655951	39.0	35.0	41.0	32.0	41.0
15	37.52043851987717	39.0	35.0	41.0	32.0	41.0
16	37.597901474269506	40.0	35.0	41.0	33.0	41.0
17	37.52601987305969	39.0	35.0	41.0	32.0	41.0
18	37.45004554229159	39.0	36.0	41.0	32.0	41.0
19	37.419886132774586	39.0	36.0	41.0	32.0	41.0
20	37.34006509607896	39.0	35.0	41.0	32.0	41.0
21	37.21009038494322	39.0	35.0	41.0	32.0	41.0
22	37.12849824088177	39.0	35.0	41.0	32.0	41.0
23	37.07096944150008	39.0	35.0	41.0	32.0	41.0
24	36.990068725666575	39.0	35.0	41.0	31.0	41.0
25	36.91795161184894	39.0	35.0	41.0	31.0	41.0
26	36.91822654079279	39.0	35.0	41.0	31.0	41.0
27	36.86917159671964	39.0	35.0	41.0	31.0	41.0
28	36.85059566295485	39.0	35.0	41.0	31.0	41.0
29	36.83199739326631	39.0	35.0	41.0	31.0	41.0
30	36.75876512560836	38.0	35.0	41.0	31.0	41.0
31	36.647600546967354	38.0	35.0	41.0	31.0	41.0
32	36.52907989461386	38.0	35.0	41.0	30.0	41.0
33	36.44596759385933	38.0	35.0	41.0	30.0	41.0
34	36.40662844814929	38.0	35.0	41.0	30.0	41.0
35	36.32968217622847	38.0	35.0	41.0	30.0	41.0
36	36.255987095091584	38.0	35.0	41.0	30.0	41.0
37	36.219806183306325	38.0	35.0	41.0	30.0	41.0
38	36.11290513834641	38.0	35.0	41.0	30.0	41.0
39	36.057562960206255	38.0	35.0	41.0	30.0	41.0
40	35.97776294555651	38.0	35.0	40.0	29.0	41.0
41	35.93553491088459	38.0	35.0	40.0	29.0	41.0
42	35.90497378320949	38.0	35.0	40.0	29.0	41.0
43	35.85716111066038	38.0	35.0	40.0	29.0	41.0
44	35.73523817156814	38.0	35.0	40.0	29.0	41.0
45	35.65103141054972	38.0	34.0	40.0	28.0	41.0
46	35.60370815750637	38.0	34.0	40.0	28.0	41.0
47	35.535120776580655	38.0	34.0	40.0	28.0	41.0
48	35.45849640473745	38.0	34.0	40.0	28.0	41.0
49	35.38352981829397	38.0	34.0	40.0	28.0	41.0
50	35.29334294216014	37.0	34.0	40.0	27.0	41.0
51	34.86011667892405	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	6.0
11	6.0
12	8.0
13	12.0
14	21.0
15	46.0
16	166.0
17	400.0
18	808.0
19	1671.0
20	3061.0
21	4946.0
22	7654.0
23	11649.0
24	17237.0
25	25271.0
26	33379.0
27	41146.0
28	47856.0
29	57194.0
30	70025.0
31	86607.0
32	108193.0
33	138204.0
34	222893.0
35	331229.0
36	227896.0
37	287412.0
38	437242.0
39	881826.0
40	356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.127007317971255	24.20323982574038	30.98307955234867	12.686673303939697
2	28.521595060870318	26.45686883852868	31.825439500358527	13.196096600242477
3	28.65886245111143	25.045829702377098	33.09743751114743	13.19787033536404
4	25.882145812194956	28.160869892258727	31.221022834211936	14.735961461334382
5	23.882128074843738	32.13692709587334	30.01455448208084	13.966390347202081
6	21.115232672989265	41.88126945565712	25.99527069661476	11.00822717473886
7	89.0593389946141	3.4255423769955753	5.719441746432739	1.7956768819575992
8	89.35584838243568	2.937305361311487	5.279489742391251	2.427356513861576
9	85.17528608869398	5.265496943098906	7.010293904624948	2.5489230635821634
10	44.08855142665786	36.23441946142175	11.148220861555703	8.528808250364683
11	30.464820427384765	24.538048753409104	29.113956897579605	15.883173921626529
12	30.351662696018263	23.228375294760287	29.154358642015254	17.2656033672062
13	23.056520069648663	30.45201011817346	28.86556828666713	17.625901525510745
14	15.849604342103577	34.90562907979607	29.742680304280977	19.502086273819373
15	15.011842966631114	29.753191327223583	38.421402019364585	16.813563686780714
16	18.885220614875134	26.694188028404724	37.071326816280134	17.34926454044001
17	19.021568290608762	26.756071675979324	28.365604911012692	25.85675512239922
18	20.078057484127534	28.728793600626457	31.7788296829974	19.414319232248605
19	23.48963990877746	28.987134836387717	27.931992367683467	19.591232887151357
20	25.124202517192913	28.86645515422791	28.058847275822053	17.950495052757123
21	23.254488617383327	28.53939810597936	29.80141064497279	18.40470263166452
22	21.366708896891133	25.63615502839126	29.355579037472783	23.641557037244823
23	19.150098393028827	29.608960384283	29.500532613240672	21.740408609447503
24	19.10614917835005	28.01299293823493	33.81596447011469	19.06489341330032
25	18.395866803003393	30.512711275667016	30.031602047415884	21.05981987391371
26	18.091704076601708	34.09424380252021	26.89393031126095	20.920121809617125
27	18.108324631629706	33.79973807844705	29.799177052597486	18.29276023732576
28	16.127095347788398	30.33655310053826	34.53465566381544	19.0016958878579
29	16.921006049422175	27.66051235324395	34.07269620548787	21.345785391846007
30	19.125528876900482	30.198497383576463	31.746705369129057	18.929268370393995
31	23.29594146411323	29.74557083559019	26.92802544193103	20.030462258365542
32	22.40959945447791	31.157004135102117	27.090420746394305	19.342975664025662
33	21.606852924183006	30.43128369480851	28.03230694289197	19.929556438116517
34	18.51359025996059	30.25046125324897	28.99646336924928	22.239485117541154
35	18.815289465360102	29.247906746204457	29.27076822110462	22.666035567330823
36	21.774733668744457	29.17850114783655	29.929053879832075	19.11771130358692
37	19.181467227123168	32.47761562700058	30.07052567925022	18.270391466626023
38	18.44093281386982	32.97114100110267	27.026402047284492	21.561524137743014
39	19.2483764575422	29.698468314028638	29.52589045608971	21.527264772339453
40	21.34969417850279	28.484445164157545	28.819582561293224	21.346278096046444
41	17.58451437267423	28.145300439524995	30.07167532238457	24.198509865416202
42	19.966246477575552	29.341389156500263	27.01066835981728	23.681696006106904
43	20.316526317137928	28.31909363449166	28.41484248410947	22.949537564260947
44	18.58194475603423	31.18173788596394	28.697687542105683	21.538629815896147
45	18.84314367615801	32.211588205712545	26.936894117538856	22.00837400059059
46	21.560308800715276	30.523419380289795	27.683800838451162	20.232470980543766
47	19.360384545774355	30.24895029370097	28.570832633967093	21.81983252655758
48	20.003790537648676	27.94276616619964	30.586879681305785	21.4665636148459
49	19.48957158712833	27.327345772910007	30.518755113859015	22.66432752610265
50	18.43708972110643	29.260519973735583	30.386841775929298	21.91554852922869
51	17.64094542709735	29.99813100873302	28.429557916229122	23.931365647940513
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3152.0
1	4330.5
2	5509.0
3	14985.5
4	24462.0
5	16718.0
6	8974.0
7	9472.5
8	9971.0
9	10844.0
10	11717.0
11	12774.5
12	13832.0
13	14651.0
14	15470.0
15	15375.5
16	15281.0
17	14766.5
18	14252.0
19	13904.5
20	13557.0
21	14722.5
22	15888.0
23	16998.0
24	18108.0
25	24227.5
26	34076.5
27	37806.0
28	49451.5
29	61097.0
30	69624.0
31	78151.0
32	86376.0
33	94601.0
34	104494.0
35	114387.0
36	118225.5
37	122064.0
38	142773.0
39	163482.0
40	192609.0
41	221736.0
42	245164.5
43	268593.0
44	284474.0
45	300355.0
46	302834.0
47	305313.0
48	287256.5
49	269200.0
50	234679.5
51	200159.0
52	180190.0
53	160221.0
54	143596.0
55	126971.0
56	110784.0
57	94597.0
58	86808.5
59	79020.0
60	69772.0
61	60524.0
62	49665.5
63	38807.0
64	31583.5
65	24360.0
66	17800.5
67	11241.0
68	8330.0
69	5419.0
70	4010.0
71	2601.0
72	2212.0
73	1823.0
74	1256.5
75	519.5
76	349.0
77	271.5
78	194.0
79	130.5
80	67.0
81	55.5
82	44.0
83	28.0
84	12.0
85	9.0
86	6.0
87	5.5
88	5.0
89	3.0
90	1.0
91	2.0
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3044423.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.98996170485371
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.9819739916786	12.22970981558395
2	11.342291180492493	3.8541018560373486
3	4.735832297947487	2.4138482814821147
4	2.436229897231703	1.6556581063274531
5	1.5203107352353176	1.2915010585563016
6	1.0084609989894693	1.0280228252201757
7	0.7575609890999754	0.9009652535729776
8	0.5194023099747843	0.7059700284707313
9	0.420731232231061	0.6433386771277476
>10	2.8818718884900596	10.267827759167995
>50	0.8414078306509686	10.365444297183947
>100	1.5354928988666279	51.062365990069594
>500	0.012942845120852165	1.463723999379517
>1k	0.005294800276712249	1.5566810684469135
>5k	0.0	0.0
>10k+	1.9610371395230552E-4	0.5608409833732908
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16833	0.5529126537278164	No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	4214	0.13841703337545405	TruSeq Adapter, Index 14 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3330	0.10938033249650261	No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	3282	0.1078036790551116	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.284694669564643E-5	0.0	0.0	0.15592445596423363	0.0
2	3.284694669564643E-5	0.0	0.0	0.4800909729035683	0.0
3	3.284694669564643E-5	0.0	0.0	0.6827566340157067	0.0
4	3.284694669564643E-5	0.0	0.0	0.9968062913727823	0.0
5	6.569389339129287E-5	0.0	0.0	1.6322304752000625	0.0
6	6.569389339129287E-5	0.0	0.0	2.1969023358449205	0.0
7	6.569389339129287E-5	0.0	0.0	2.57897801980868	0.0
8	6.569389339129287E-5	0.0	0.0	3.495999077657737	0.0
9	6.569389339129287E-5	0.0	0.0	3.788731066609338	0.0
10	6.569389339129287E-5	0.0	0.0	4.332873585569416	0.0
11	6.569389339129287E-5	0.0	0.0	5.238595293755171	0.0
12	6.569389339129287E-5	0.0	0.0	5.93741408470505	0.0
13	6.569389339129287E-5	0.0	0.0	6.202850260952568	0.0
14	6.569389339129287E-5	0.0	0.0	6.303723234254898	0.0
15	6.569389339129287E-5	0.0	0.0	6.460436016939827	0.0
16	6.569389339129287E-5	0.0	0.0	6.817580868361591	0.0
17	6.569389339129287E-5	0.0	0.0	7.294847003849334	0.0
18	6.569389339129287E-5	0.0	0.0	7.857088190438714	0.0
19	9.854084008693929E-5	0.0	0.0	8.214397276593955	0.0
20	9.854084008693929E-5	0.0	0.0	8.557713563456852	0.0
21	9.854084008693929E-5	0.0	0.0	8.958643394823913	0.0
22	9.854084008693929E-5	0.0	0.0	9.429997079906439	0.0
23	9.854084008693929E-5	0.0	0.0	9.893598885568792	0.0
24	9.854084008693929E-5	0.0	0.0	10.243977265971253	0.0
25	1.3138778678258573E-4	0.0	0.0	10.537399040803463	0.0
26	1.3138778678258573E-4	0.0	0.0	10.818568904518196	0.0
27	1.3138778678258573E-4	0.0	0.0	11.101118339994146	0.0
28	1.3138778678258573E-4	0.0	0.0	11.41359134390983	0.0
29	1.3138778678258573E-4	0.0	0.0	11.760553641855944	0.0
30	1.3138778678258573E-4	0.0	0.0	12.160498064822136	0.0
31	1.3138778678258573E-4	0.0	0.0	12.516756048683117	0.0
32	1.3138778678258573E-4	0.0	0.0	12.850579567950971	0.0
33	1.3138778678258573E-4	0.0	0.0	13.17895049406735	0.0
34	1.3138778678258573E-4	0.0	0.0	13.511887145774422	0.0
35	1.3138778678258573E-4	0.0	0.0	13.894784003405572	0.0
36	1.6423473347823217E-4	0.0	0.0	14.264279306784898	0.0
37	1.6423473347823217E-4	0.0	0.0	14.628124935332574	0.0
38	1.6423473347823217E-4	0.0	0.0	14.997488850925118	0.0
39	1.6423473347823217E-4	0.0	0.0	15.38271784177166	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATC	30	2.1666856E-6	45.000004	31
TCGCTAG	60	0.0	45.000004	1
CGTAAAC	30	2.1666856E-6	45.000004	15
CGTCGCA	55	1.8189894E-12	45.000004	31
CGCCGGT	60	0.0	45.000004	28
AGTCCGC	20	7.035121E-4	45.0	34
GTCGAGT	20	7.035121E-4	45.0	2
CGAAACA	20	7.035121E-4	45.0	44
ACGTTAC	25	3.892327E-5	45.0	45
CGTGACG	25	3.892327E-5	45.0	11
CGCGACA	25	3.892327E-5	45.0	19
CCCGTAC	25	3.892327E-5	45.0	27
GTCACGA	20	7.035121E-4	45.0	45
TCGTCAA	25	3.892327E-5	45.0	21
CCCGGTA	20	7.035121E-4	45.0	29
CAATCGT	25	3.892327E-5	45.0	40
ATCGCAA	40	6.8212103E-9	45.0	29
TAGTCGT	20	7.035121E-4	45.0	28
AATGTCG	20	7.035121E-4	45.0	15
AGTCGTG	40	6.8212103E-9	45.0	1
>>END_MODULE