FastQCFastQC Report
Thu 26 May 2016
SRR1547537_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547537_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2980954
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT474021.5901620756308215No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT34630.11617086342157579Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG406.8212103E-945.039
CGTTAGT253.8923077E-545.039
CGTTACG207.035098E-445.018
CGTACCG207.035098E-445.012
TCCGTAC207.035098E-445.011
CGTAACG253.8923077E-545.037
CCCGGTA253.8923077E-545.028
CGGTCTA7000.044.03571331
CGTTTTT194400.043.5069431
TCGTTAG1300.043.269231
CGACGGT7100.043.0985928
ACGATAT1900.042.6315818
CACGACG7350.041.93877426
ATATACG603.6379788E-1241.2499961
ACGGTCT7600.040.5592130
CACGATA501.0822987E-940.522
GTCGGTT501.0822987E-940.58
TATAGCG900.040.01
ACGACGG7750.039.77419327
AGGGATC26900.039.6468436