##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547537_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2980954
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45563500812156	31.0	31.0	33.0	30.0	34.0
2	31.870970165926746	31.0	31.0	34.0	30.0	34.0
3	31.895112772622454	31.0	31.0	34.0	30.0	34.0
4	35.70643089427076	37.0	35.0	37.0	33.0	37.0
5	35.650531339967	37.0	35.0	37.0	33.0	37.0
6	35.6982824961405	37.0	35.0	37.0	33.0	37.0
7	36.03404413486421	37.0	35.0	37.0	35.0	37.0
8	35.986399320485994	37.0	35.0	37.0	35.0	37.0
9	37.64365334050777	39.0	38.0	39.0	35.0	39.0
10	37.22490048487833	39.0	37.0	39.0	34.0	39.0
11	37.02715171049268	39.0	37.0	39.0	33.0	39.0
12	36.644189075041076	39.0	35.0	39.0	33.0	39.0
13	36.477645076039416	39.0	35.0	39.0	32.0	39.0
14	37.52828524022846	40.0	36.0	41.0	32.0	41.0
15	37.736666516826496	40.0	36.0	41.0	33.0	41.0
16	37.81384449407807	40.0	36.0	41.0	33.0	41.0
17	37.758896983985665	40.0	36.0	41.0	33.0	41.0
18	37.62148325670238	39.0	36.0	41.0	33.0	41.0
19	37.55241140923342	39.0	36.0	41.0	33.0	41.0
20	37.373600867373334	39.0	35.0	41.0	33.0	41.0
21	37.25000251597307	39.0	35.0	41.0	32.0	41.0
22	37.192550103087804	39.0	35.0	41.0	32.0	41.0
23	37.117856565381416	39.0	35.0	41.0	32.0	41.0
24	37.02341297450413	39.0	35.0	41.0	32.0	41.0
25	36.91393594131275	38.0	35.0	41.0	32.0	41.0
26	36.91632242563958	38.0	35.0	41.0	32.0	41.0
27	36.87664888488719	38.0	35.0	41.0	32.0	41.0
28	36.79836723411364	38.0	35.0	41.0	32.0	41.0
29	36.74994884188082	38.0	35.0	41.0	32.0	41.0
30	36.62790133628362	38.0	35.0	41.0	31.0	41.0
31	36.445595940091664	38.0	35.0	40.0	31.0	41.0
32	36.22015502419695	38.0	35.0	41.0	30.0	41.0
33	36.0065432073088	38.0	35.0	41.0	30.0	41.0
34	35.81792775064627	38.0	35.0	41.0	29.0	41.0
35	35.63274978412951	38.0	35.0	41.0	28.0	41.0
36	35.53563523623645	38.0	35.0	41.0	27.0	41.0
37	35.45483660599929	38.0	35.0	40.0	27.0	41.0
38	35.32137865931511	38.0	35.0	40.0	26.0	41.0
39	35.25208138065867	38.0	35.0	40.0	26.0	41.0
40	35.16255031107491	38.0	34.0	40.0	25.0	41.0
41	35.07710216259627	38.0	34.0	40.0	25.0	41.0
42	35.042968459090616	38.0	34.0	40.0	25.0	41.0
43	34.989913296213224	38.0	34.0	40.0	24.0	41.0
44	34.892935952718496	38.0	34.0	40.0	24.0	41.0
45	34.83277434002672	37.0	34.0	40.0	24.0	41.0
46	34.79252346731952	37.0	34.0	40.0	24.0	41.0
47	34.70977479021816	37.0	34.0	40.0	24.0	41.0
48	34.6003232522206	37.0	34.0	40.0	23.0	41.0
49	34.51796975062346	37.0	34.0	40.0	24.0	41.0
50	34.42044526685081	37.0	34.0	40.0	24.0	41.0
51	34.04404093454646	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	8.0
11	6.0
12	10.0
13	13.0
14	24.0
15	77.0
16	172.0
17	419.0
18	1010.0
19	2015.0
20	3556.0
21	5801.0
22	9214.0
23	14416.0
24	22368.0
25	35493.0
26	47648.0
27	52132.0
28	54229.0
29	58114.0
30	66519.0
31	80392.0
32	101011.0
33	132984.0
34	218776.0
35	309867.0
36	249108.0
37	290756.0
38	428045.0
39	796521.0
40	247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.32252292386934	23.339407451440042	29.27384991516139	16.064219709529233
2	34.34377048421411	23.724418424437278	29.275762054697925	12.656049036650682
3	28.1741684038063	23.928145150847683	34.60653871210357	13.291147733242445
4	26.034450716113028	25.780203250368842	34.20160123235716	13.983744801160972
5	23.19297781851045	29.890498142540945	32.69034007233926	14.226183966609346
6	21.072851174489777	38.58298383671804	29.84960519350517	10.494559795287012
7	83.48347542431047	3.47197575004512	10.77363152869853	2.270917296945877
8	83.51799457489113	3.2214519244510313	10.545885646004601	2.714667854653242
9	78.10013170280386	5.737794008226897	12.452456495470914	3.709617793498323
10	38.80173930895948	30.797925764704857	18.747018571906846	11.653316354428817
11	30.455015407819108	24.095608318679187	29.01883088434105	16.43054538916065
12	28.673840656380474	21.078285676330463	32.556121295397375	17.691752371891685
13	23.306028875319782	27.084785608902383	32.40912137523759	17.200064140540245
14	17.610469668435005	30.999102971733212	33.10789096376529	18.282536396066497
15	16.613607590053387	28.380142732829828	38.62015314560372	16.38609653151307
16	20.550233247477152	26.459415341531606	36.37251698617288	16.617834424818366
17	20.438691774512456	26.847076472833862	30.307478746736784	22.406753005916897
18	21.730660721366384	27.436518644702335	33.0914532730126	17.741367360918687
19	22.84013775455777	29.41216134163761	28.976495444075955	18.771205459728666
20	23.829418367408557	28.561896627723875	29.442118194376697	18.16656681049087
21	22.314232289394603	28.395741765891053	32.16980201640146	17.120223928312882
22	21.409320640305083	25.323638003135905	32.298955300886895	20.96808605567211
23	19.000058370575328	28.6941361725139	31.137347305594115	21.16845815131666
24	19.192983185919676	28.41425932771858	33.93175473355174	18.461002752810007
25	19.503789726376187	28.99202738452187	31.220810519048598	20.283372370053346
26	17.710638943103447	31.663118585526647	30.22861137743152	20.397631093938383
27	18.211250492292063	30.850660560344107	32.662362451752024	18.275726495611806
28	16.77020175420352	29.63084972126373	34.587752779814785	19.011195744717966
29	17.96991835499642	28.185037407487666	33.88931865436367	19.95572558315224
30	19.54310599895201	28.615939729361806	32.947640252080376	18.893314019605803
31	22.225535851945384	28.533851914521325	29.849135545197946	19.391476688335345
32	22.862244771304756	29.33366298171659	29.48737216340809	18.316720083570562
33	22.327013432612514	28.692056301439067	29.308738075126286	19.672192190822134
34	19.076879415113417	29.33138183279581	31.214403174285817	20.37733557780496
35	20.355496931519237	28.91024148645031	30.35779820822462	20.376463373805837
36	22.930578600005234	28.766696835979356	29.297969710367887	19.004754853647523
37	20.234864409179075	31.656878972302156	29.87721380470816	18.231042813810614
38	20.455565567264706	31.010475170029462	27.77432996282398	20.759629299881848
39	19.947808654544822	29.315044780966094	29.39948083734268	21.33766572714641
40	22.015200502926245	28.12331220139593	28.83697635052403	21.0245109451538
41	19.219853778354178	27.524477063383063	30.350384474232072	22.905284684030683
42	20.274381959600856	28.63180713288431	29.787041329721966	21.30676957779288
43	19.953746351000383	28.51043659177565	30.484334880712684	21.05148217651128
44	19.936067446864325	29.999523642431246	28.73214413909104	21.33226477161338
45	19.285101346750068	30.897021557528227	27.918142983756205	21.8997341119655
46	21.38553630817517	29.787242607567915	28.440660271845857	20.386560812411062
47	19.608487752578537	29.325880238339806	30.14692611828294	20.918705890798716
48	20.179814918311386	28.42234398786429	30.770887440732057	20.626953653092265
49	20.543154976561194	27.80495774171624	30.23045642435274	21.42143085736982
50	18.891670250530535	29.218129498140527	30.616205416118465	21.273994835210473
51	18.376801520587033	30.26162094416754	28.752506747839785	22.609070787405642
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3317.0
1	4771.5
2	6226.0
3	35551.0
4	64876.0
5	42715.0
6	20554.0
7	21414.5
8	22275.0
9	25056.0
10	27837.0
11	30484.0
12	33131.0
13	33590.0
14	34049.0
15	31458.0
16	28867.0
17	26440.5
18	24014.0
19	22596.5
20	21179.0
21	20680.5
22	20182.0
23	20795.0
24	21408.0
25	24446.5
26	30392.0
27	33299.0
28	39702.0
29	46105.0
30	50765.0
31	55425.0
32	66476.0
33	77527.0
34	85008.0
35	92489.0
36	102578.0
37	112667.0
38	117753.0
39	122839.0
40	145052.0
41	167265.0
42	183190.5
43	199116.0
44	208872.5
45	218629.0
46	237858.5
47	257088.0
48	260277.0
49	263466.0
50	246338.0
51	229210.0
52	201676.5
53	174143.0
54	159534.5
55	144926.0
56	133051.5
57	121177.0
58	111115.0
59	101053.0
60	88395.0
61	75737.0
62	63240.5
63	50744.0
64	41587.0
65	32430.0
66	27196.5
67	21963.0
68	16937.0
69	11911.0
70	9292.5
71	6674.0
72	5618.0
73	4562.0
74	3459.0
75	2034.5
76	1713.0
77	1125.5
78	538.0
79	417.5
80	297.0
81	194.5
82	92.0
83	79.5
84	67.0
85	36.5
86	6.0
87	12.0
88	18.0
89	13.5
90	9.0
91	8.0
92	7.0
93	5.5
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2980954.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.63553225966592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.13887684985473	16.256695940400963
2	9.916432120488409	4.290945740872286
3	3.8213640483382814	2.480317354312513
4	2.061620599019277	1.7841703590909335
5	1.2856001349968071	1.3907321596877142
6	0.9101913971824906	1.18154852017273
7	0.7046262463723693	1.067147471907769
8	0.5299510371802919	0.9172618208766095
9	0.44879867031178183	0.8738998298663153
>10	3.1982077964093225	14.414940597643
>50	0.8998809236730793	14.243425702482334
>100	1.0759645402871587	37.29884763622026
>500	0.004557100753744398	0.6839114007608295
>1k	0.0037713937272367435	1.5045642836185
>5k	0.0	0.0
>10k+	1.5714140530153096E-4	1.6115911820871365
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47402	1.5901620756308215	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	3463	0.11617086342157579	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3546307658554944E-5	0.0	0.0	0.12667085771870348	0.0
2	3.3546307658554944E-5	0.0	0.0	0.37903973023401233	0.0
3	3.3546307658554944E-5	0.0	0.0	0.593199358326227	0.0
4	3.3546307658554944E-5	0.0	0.0	0.8376848485417756	0.0
5	3.3546307658554944E-5	0.0	0.0	1.4178346931888248	0.0
6	3.3546307658554944E-5	0.0	0.0	2.113551567719596	0.0
7	3.3546307658554944E-5	0.0	0.0	2.603394752149815	0.0
8	3.3546307658554944E-5	0.0	0.0	3.505186594627089	0.0
9	3.3546307658554944E-5	0.0	0.0	3.910560176373067	0.0
10	3.3546307658554944E-5	0.0	0.0	4.542371334814291	0.0
11	3.3546307658554944E-5	0.0	0.0	5.336345344476969	0.0
12	3.3546307658554944E-5	0.0	0.0	5.92736419280539	0.0
13	3.3546307658554944E-5	0.0	0.0	6.188253827465973	0.0
14	3.3546307658554944E-5	0.0	0.0	6.320493372255996	0.0
15	3.3546307658554944E-5	0.0	0.0	6.478194564558863	0.0
16	3.3546307658554944E-5	0.0	0.0	6.7978908765448915	0.0
17	3.3546307658554944E-5	0.0	0.0	7.186692582307543	0.0
18	3.3546307658554944E-5	0.0	0.0	7.654395203683116	0.0
19	3.3546307658554944E-5	0.0	0.0	7.934842335708636	0.0
20	3.3546307658554944E-5	0.0	0.0	8.214886912042251	0.0
21	3.3546307658554944E-5	0.0	0.0	8.580038470905622	0.0
22	3.3546307658554944E-5	0.0	0.0	8.948544660534848	0.0
23	3.3546307658554944E-5	0.0	0.0	9.348785657209069	0.0
24	3.3546307658554944E-5	0.0	0.0	9.647582619523817	0.0
25	3.3546307658554944E-5	0.0	0.0	9.909008995106936	0.0
26	3.3546307658554944E-5	0.0	0.0	10.166476906386345	0.0
27	3.3546307658554944E-5	0.0	0.0	10.449540650409231	0.0
28	3.3546307658554944E-5	0.0	0.0	10.731832829355971	0.0
29	3.3546307658554944E-5	0.0	0.0	11.043813490580533	0.0
30	3.3546307658554944E-5	0.0	0.0	11.394607229766041	0.0
31	3.3546307658554944E-5	0.0	0.0	11.725541554817687	0.0
32	3.3546307658554944E-5	0.0	0.0	12.020212321290433	0.0
33	3.3546307658554944E-5	0.0	0.0	12.31719778299162	0.0
34	3.3546307658554944E-5	0.0	0.0	12.617269504997394	0.0
35	3.3546307658554944E-5	0.0	0.0	12.945184662359768	0.0
36	3.3546307658554944E-5	0.0	0.0	13.252636572050424	0.0
37	3.3546307658554944E-5	0.0	0.0	13.586690703714314	0.0
38	6.709261531710989E-5	0.0	0.0	13.94798443719695	0.0
39	6.709261531710989E-5	0.0	0.0	14.47194421651592	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCG	40	6.8212103E-9	45.0	39
CGTTAGT	25	3.8923077E-5	45.0	39
CGTTACG	20	7.035098E-4	45.0	18
CGTACCG	20	7.035098E-4	45.0	12
TCCGTAC	20	7.035098E-4	45.0	11
CGTAACG	25	3.8923077E-5	45.0	37
CCCGGTA	25	3.8923077E-5	45.0	28
CGGTCTA	700	0.0	44.035713	31
CGTTTTT	19440	0.0	43.506943	1
TCGTTAG	130	0.0	43.26923	1
CGACGGT	710	0.0	43.09859	28
ACGATAT	190	0.0	42.63158	18
CACGACG	735	0.0	41.938774	26
ATATACG	60	3.6379788E-12	41.249996	1
ACGGTCT	760	0.0	40.55921	30
CACGATA	50	1.0822987E-9	40.5	22
GTCGGTT	50	1.0822987E-9	40.5	8
TATAGCG	90	0.0	40.0	1
ACGACGG	775	0.0	39.774193	27
AGGGATC	2690	0.0	39.646843	6
>>END_MODULE