FastQCFastQC Report
Thu 26 May 2016
SRR1547536_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547536_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1846298
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77000.41705076861915036No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41260.223474217054885No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23740.12858162658465752No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCG207.034398E-445.00000440
AATCCCG650.045.00000432
GGTACGA207.034398E-445.0000048
ACACGAT207.034398E-445.00000415
CACGGTA207.034398E-445.00000411
CAAGTCG800.045.00000426
CGCATTA406.8193913E-945.00000431
CGTGACG302.16622E-645.0000042
TAGCGGA600.045.0000043
CACGCTA302.16622E-645.00000432
ACCGACA650.045.00000427
TCCTCGA207.034398E-445.00000443
CCGTCTG302.16622E-645.00000437
ATATCGT302.16622E-645.00000423
CGGATAT207.034398E-445.0000046
CCGAGTC207.034398E-445.00000425
TCTCGAG406.8193913E-945.0000041
AACGCGC207.034398E-445.00000429
TTGCGCA650.045.00000412
GCGATAC800.045.0000049