FastQCFastQC Report
Thu 26 May 2016
SRR1547535_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547535_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences691129
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138171.999192625399889No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTT10250.14830805826408672No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC8310.120238045285323No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGTC1100.045.016
GCGATTA850.045.09
ACCACGC207.0313324E-445.026
CGGTCTA1100.045.031
GCGTAAG302.1642409E-644.9999961
TACGTAG302.1642409E-644.9999961
CGTTTTT70500.043.5319181
ACGGTCT1150.043.04347630
TCACGAC1200.041.24999625
TACGGGT556.002665E-1140.9090924
TAGGGAT8750.039.6000025
TGCGTCT1250.039.617
GTAGGGA9000.039.04
CACGACG1400.038.57142626
CGAGGGA3500.038.5714264
TATCGCG356.2446743E-638.5714261
TAGGGAC6600.038.5227285
AAGGGAC7600.038.4868435
AGGGATT10150.038.3497546
TAGGGAG6400.038.3203125
Produced by FastQC (version 0.11.5)