##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547535_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	691129
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.343119735968248	31.0	31.0	33.0	30.0	34.0
2	31.7540198718329	31.0	31.0	34.0	30.0	34.0
3	31.706348597729225	31.0	31.0	34.0	30.0	34.0
4	35.58904343472781	37.0	35.0	37.0	33.0	37.0
5	35.558545510317174	37.0	35.0	37.0	33.0	37.0
6	35.605302338637216	37.0	35.0	37.0	33.0	37.0
7	35.9401544429477	37.0	35.0	37.0	35.0	37.0
8	35.88236349509281	37.0	35.0	37.0	35.0	37.0
9	37.5353978779649	39.0	37.0	39.0	35.0	39.0
10	37.118921359109514	39.0	37.0	39.0	33.0	39.0
11	36.905459038761215	39.0	37.0	39.0	32.0	39.0
12	36.635503646931326	39.0	35.0	39.0	32.0	39.0
13	36.57161253543116	39.0	35.0	39.0	32.0	39.0
14	37.705947804244936	40.0	36.0	41.0	32.0	41.0
15	37.799584448055285	40.0	36.0	41.0	33.0	41.0
16	37.81649156669739	40.0	36.0	41.0	33.0	41.0
17	37.70268358005524	40.0	36.0	41.0	32.0	41.0
18	37.50545556618229	39.0	36.0	41.0	32.0	41.0
19	37.36222470768843	39.0	36.0	41.0	32.0	41.0
20	37.14775678635971	39.0	35.0	41.0	32.0	41.0
21	37.026669406145594	39.0	35.0	41.0	32.0	41.0
22	36.98266604353167	39.0	35.0	41.0	32.0	41.0
23	36.90495985554072	38.0	35.0	41.0	31.0	41.0
24	36.8069984040606	38.0	35.0	41.0	31.0	41.0
25	36.71456848142677	38.0	35.0	41.0	31.0	41.0
26	36.70419993951925	38.0	35.0	41.0	31.0	41.0
27	36.65866574836246	38.0	35.0	41.0	31.0	41.0
28	36.54288707318026	38.0	35.0	40.0	31.0	41.0
29	36.45856706924467	38.0	35.0	40.0	31.0	41.0
30	36.28407142516086	38.0	35.0	40.0	30.0	41.0
31	36.053265019989034	38.0	35.0	40.0	30.0	41.0
32	35.71890197054385	38.0	35.0	40.0	27.0	41.0
33	35.38015334329771	38.0	34.0	41.0	25.0	41.0
34	35.0582988125227	38.0	34.0	41.0	23.0	41.0
35	34.82598617624206	38.0	34.0	41.0	23.0	41.0
36	34.75284787644564	38.0	34.0	40.0	22.0	41.0
37	34.71619914661373	38.0	34.0	40.0	22.0	41.0
38	34.581642500893466	38.0	34.0	40.0	21.0	41.0
39	34.5022607935711	38.0	34.0	40.0	21.0	41.0
40	34.39016594586539	38.0	34.0	40.0	20.0	41.0
41	34.27138348991288	38.0	33.0	40.0	20.0	41.0
42	34.22340547133748	38.0	33.0	40.0	19.0	41.0
43	34.15684915551221	38.0	33.0	40.0	19.0	41.0
44	34.01898777218146	38.0	33.0	40.0	18.0	41.0
45	33.937256286453035	37.0	33.0	40.0	19.0	41.0
46	33.89783238729673	37.0	33.0	40.0	19.0	41.0
47	33.83312666665702	37.0	33.0	40.0	19.0	41.0
48	33.716492868914486	37.0	33.0	40.0	18.0	41.0
49	33.63810084658581	37.0	33.0	40.0	18.0	41.0
50	33.55337136771862	37.0	33.0	40.0	18.0	41.0
51	33.15832066083177	36.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	1.0
13	6.0
14	8.0
15	22.0
16	56.0
17	158.0
18	361.0
19	765.0
20	1279.0
21	2008.0
22	3110.0
23	4440.0
24	6943.0
25	10858.0
26	15294.0
27	17539.0
28	17714.0
29	17273.0
30	18073.0
31	20605.0
32	23804.0
33	29749.0
34	45777.0
35	57467.0
36	51535.0
37	64766.0
38	100061.0
39	181409.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.666055106933726	22.558017388938968	26.230124911557755	20.545802592569547
2	37.37898424172622	22.957942728492075	27.09132448500931	12.571748544772394
3	27.206498352695373	22.88487388027416	36.74856647601244	13.16006129101803
4	24.4659101267636	25.07057293211542	36.116701802413154	14.346815138707825
5	22.39046545579769	29.057816992196827	34.3775185240382	14.174199027967283
6	21.0309508065788	37.0851172501805	31.379814766852498	10.5041171763882
7	80.36994540816548	3.2506232555716807	13.995650594896178	2.3837807413666625
8	80.86565604973892	2.6644808711542995	14.084635429854627	2.385227649252166
9	75.55738509019301	5.348350307974344	15.611846703003346	3.482417898829307
10	36.37946027442055	31.727362040950386	21.06176994454002	10.831407740089043
11	25.96765582112746	22.597083901847554	33.33140412281933	18.103856154205655
12	25.744687315971404	19.737415156938866	36.13232840757659	18.385569119513146
13	23.6071702967174	20.21156687101829	36.385681978328215	19.795580853936094
14	17.818381228395857	25.024850642933522	37.338181439355026	19.818586689315598
15	17.70147107124719	26.071977879672247	39.1976027630153	17.028948286065265
16	22.969952063941754	24.67354140833332	37.06630744766896	15.290199080055967
17	22.001102543808752	24.997070011531854	34.532771740152704	18.469055704506683
18	21.732411749470792	26.145336109467266	34.49819064168918	17.624061499372765
19	22.23651445678014	28.03919384080251	32.60983116031884	17.11446054209851
20	24.211543720492124	26.880654696880036	32.01225820360598	16.89554337902186
21	24.581083994449664	25.05711668878024	34.09638432188491	16.26541499488518
22	22.1981713978143	22.55324259291681	35.443600254077026	19.80498575519187
23	20.470852764100478	24.82156008502031	35.16883244661995	19.53875470425926
24	20.78338486736919	24.77511434189565	35.60160259517398	18.839898195561176
25	21.826171380451406	25.425210054852275	32.66206453498551	20.086554029710808
26	20.1285143583904	27.73288344144147	32.533434423964266	19.605167776203864
27	18.962161911886202	26.21811557610808	35.19748122275291	19.622241289252802
28	18.23856327834601	26.43037696291141	35.641392562025324	19.689667196717256
29	20.06586324694811	26.12594754380152	34.05427930241677	19.7539099068336
30	20.796117656761616	24.191431700883626	34.515987608680874	20.496463033673887
31	21.66961594723995	25.008066511461678	33.13071799910002	20.191599542198347
32	22.418390777987902	26.27758349020226	32.2172850509818	19.086740680828036
33	21.825158544931554	25.764365263214252	31.75745772496886	20.653018466885342
34	19.764761715974878	26.911907907206906	32.38136440519787	20.94196597162035
35	19.755935577873306	27.15180523462335	31.402820602232	21.689438585271343
36	20.325583212395948	28.67293949465295	30.439324641275363	20.562152651675735
37	21.103440891642517	28.920216052285465	29.424029378017707	20.552313678054315
38	20.440033626139257	28.908351407624338	29.16069214285611	21.490922823380295
39	20.51049804016327	27.229359497286325	28.982722472939205	23.2774199896112
40	21.33523553490014	26.076173912540206	30.506316476374163	22.082274076185488
41	18.72110705816136	26.942292972802473	31.07798978193651	23.25861018709966
42	19.672304302091213	28.903865993179277	29.189919682143277	22.23391002258623
43	20.092341661252817	27.14399193204163	30.45104459514794	22.312621811557612
44	20.752421038619417	27.74199896112014	29.850433131875526	21.655146868384918
45	21.032542405252855	26.864738710139495	29.7083467775191	22.394372107088547
46	20.745186499191902	27.886545058881918	29.691128573681613	21.67713986824457
47	20.846470051177132	27.369709562180145	30.12158366961884	21.662236717023884
48	19.918278642626774	26.428495982660255	31.490358529304952	22.162866845408022
49	20.519758250630492	26.12146212935646	31.104323505452676	22.254456114560377
50	18.787375439317408	26.529056080702734	32.23392449166509	22.449643988314772
51	18.618955361444826	26.461774864026832	32.54746943045365	22.371800344074696
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	415.0
1	770.0
2	1125.0
3	11593.5
4	22062.0
5	15068.5
6	8075.0
7	7687.5
8	7300.0
9	7335.5
10	7371.0
11	7493.0
12	7615.0
13	7303.5
14	6992.0
15	6689.5
16	6387.0
17	5988.5
18	5590.0
19	5396.5
20	5203.0
21	5057.0
22	4911.0
23	5181.0
24	5451.0
25	5814.5
26	7186.0
27	8194.0
28	9193.5
29	10193.0
30	11722.5
31	13252.0
32	14713.5
33	16175.0
34	18064.0
35	19953.0
36	21547.5
37	23142.0
38	24743.0
39	26344.0
40	28594.5
41	30845.0
42	32894.5
43	34944.0
44	37187.5
45	39431.0
46	41343.5
47	43256.0
48	46804.0
49	50352.0
50	51420.5
51	52489.0
52	50310.0
53	48131.0
54	43606.0
55	39081.0
56	37108.5
57	35136.0
58	34029.0
59	32922.0
60	29949.0
61	26976.0
62	23255.5
63	19535.0
64	15557.0
65	11579.0
66	9042.0
67	6505.0
68	5004.0
69	3503.0
70	2803.5
71	2104.0
72	1630.0
73	1156.0
74	925.0
75	499.0
76	304.0
77	218.5
78	133.0
79	97.0
80	61.0
81	45.5
82	30.0
83	21.5
84	13.0
85	13.0
86	13.0
87	8.0
88	3.0
89	3.5
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	691129.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.72276669184246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.62963779933959	26.26622581541982
2	8.930684488374974	6.737802547068511
3	4.6796376601963825	5.29586638973843
4	3.2081383228346896	4.84079413869807
5	2.3970122345270624	4.521096664027817
6	1.8190891007632437	4.11726442438592
7	1.4969944469162804	3.9529540582004605
8	1.239579308050379	3.7408288826895966
9	0.9912708613556129	3.3654131488205543
>10	5.558852881105704	32.43897982000809
>50	0.028997774185924925	0.731730292051692
>100	0.01701202660395682	1.2773557142905643
>500	0.002319821809630468	0.5489800078316598
>1k	3.866369682717447E-4	0.14949641552270226
>5k	0.0	0.0
>10k+	3.866369682717447E-4	2.0152116812460266
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13817	1.999192625399889	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTT	1025	0.14830805826408672	No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC	831	0.120238045285323	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4469078855032852E-4	0.0	0.0	0.09679813754016978	0.0
2	1.4469078855032852E-4	0.0	0.0	0.2435145971302029	0.0
3	1.4469078855032852E-4	0.0	0.0	0.4220630302013083	0.0
4	1.4469078855032852E-4	0.0	0.0	0.5767374831616094	0.0
5	1.4469078855032852E-4	0.0	0.0	0.9886721581643948	0.0
6	1.4469078855032852E-4	0.0	0.0	1.8108052187073613	0.0
7	1.4469078855032852E-4	0.0	0.0	2.3477527350176306	0.0
8	1.4469078855032852E-4	0.0	0.0	3.0934890592060236	0.0
9	1.4469078855032852E-4	0.0	0.0	3.5960001678413147	0.0
10	1.4469078855032852E-4	0.0	0.0	4.126291907878269	0.0
11	1.4469078855032852E-4	0.0	0.0	4.574109898441535	0.0
12	1.4469078855032852E-4	0.0	0.0	4.886207929344594	0.0
13	1.4469078855032852E-4	0.0	0.0	5.066058579512653	0.0
14	1.4469078855032852E-4	0.0	0.0	5.192952401071291	0.0
15	1.4469078855032852E-4	0.0	0.0	5.293512499113769	0.0
16	1.4469078855032852E-4	0.0	0.0	5.421129774615158	0.0
17	1.4469078855032852E-4	0.0	0.0	5.5650971092227355	0.0
18	1.4469078855032852E-4	0.0	0.0	5.740462344945734	0.0
19	1.4469078855032852E-4	0.0	0.0	5.86200260732801	0.0
20	1.4469078855032852E-4	0.0	0.0	5.9941052972744595	0.0
21	1.4469078855032852E-4	0.0	0.0	6.1440049542126	0.0
22	1.4469078855032852E-4	0.0	0.0	6.318067972838645	0.0
23	1.4469078855032852E-4	0.0	0.0	6.502548728240314	0.0
24	1.4469078855032852E-4	0.0	0.0	6.646660753636441	0.0
25	1.4469078855032852E-4	0.0	0.0	6.784261693547804	0.0
26	1.4469078855032852E-4	0.0	0.0	6.9250458308072735	0.0
27	1.4469078855032852E-4	0.0	0.0	7.066408731220944	0.0
28	1.4469078855032852E-4	0.0	0.0	7.218044677621688	0.0
29	1.4469078855032852E-4	0.0	0.0	7.363458920114769	0.0
30	1.4469078855032852E-4	0.0	0.0	7.540994517666022	0.0
31	1.4469078855032852E-4	0.0	0.0	7.70782299686455	0.0
32	1.4469078855032852E-4	0.0	0.0	7.8630762129790535	0.0
33	1.4469078855032852E-4	0.0	0.0	8.032364435582938	0.0
34	1.4469078855032852E-4	0.0	0.0	8.214819519944902	0.0
35	1.4469078855032852E-4	0.0	0.0	8.400457801654973	0.0
36	1.4469078855032852E-4	0.0	0.0	8.620387800251473	0.0
37	1.4469078855032852E-4	0.0	0.0	8.82613810157004	0.0
38	2.8938157710065704E-4	0.0	0.0	9.072257132894148	0.0
39	2.8938157710065704E-4	0.0	0.0	9.359613038955102	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGTC	110	0.0	45.0	16
GCGATTA	85	0.0	45.0	9
ACCACGC	20	7.0313324E-4	45.0	26
CGGTCTA	110	0.0	45.0	31
GCGTAAG	30	2.1642409E-6	44.999996	1
TACGTAG	30	2.1642409E-6	44.999996	1
CGTTTTT	7050	0.0	43.531918	1
ACGGTCT	115	0.0	43.043476	30
TCACGAC	120	0.0	41.249996	25
TACGGGT	55	6.002665E-11	40.909092	4
TAGGGAT	875	0.0	39.600002	5
TGCGTCT	125	0.0	39.6	17
GTAGGGA	900	0.0	39.0	4
CACGACG	140	0.0	38.571426	26
CGAGGGA	350	0.0	38.571426	4
TATCGCG	35	6.2446743E-6	38.571426	1
TAGGGAC	660	0.0	38.522728	5
AAGGGAC	760	0.0	38.486843	5
AGGGATT	1015	0.0	38.349754	6
TAGGGAG	640	0.0	38.320312	5
>>END_MODULE