FastQCFastQC Report
Thu 26 May 2016
SRR1547534_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547534_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2350470
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87990.37435066178253706No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT38180.1624355979867856TruSeq Adapter, Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC29370.12495373265772378Illumina PCR Primer Index 2 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26590.11312631090803116No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC24660.1049151871753309RNA PCR Primer, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATCG253.8920538E-545.00000418
CGCGTAC253.8920538E-545.00000410
CAATCGA351.2126475E-745.00000422
TATCGTG453.8562575E-1045.0000041
GCGTACT253.8920538E-545.00000411
AGTCGAC502.1827873E-1145.00000431
CGCTAGC207.0347934E-445.042
TGTTACG1750.045.01
CGTACAC207.0347934E-445.044
CCGGTTA207.0347934E-445.032
TTCGCTA207.0347934E-445.012
CCCGAAT207.0347934E-445.034
CGACATA302.1664746E-644.99999610
GTTACGA302.1664746E-644.99999641
CTCGACC302.1664746E-644.99999617
CACGACG8900.043.9887626
CGACGGT9200.042.79891228
CGGTCTA9350.042.35294331
TAGGGTA12450.041.927715
TCCAACG700.041.78571710
Produced by FastQC (version 0.11.5)