##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547534_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2350470
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.399201436308484	31.0	31.0	33.0	30.0	34.0
2	31.809877598948294	31.0	31.0	34.0	30.0	34.0
3	31.969371657583377	31.0	31.0	34.0	30.0	34.0
4	35.690188770756485	37.0	35.0	37.0	33.0	37.0
5	35.59212668104677	37.0	35.0	37.0	33.0	37.0
6	35.626502359102645	37.0	35.0	37.0	33.0	37.0
7	35.97800014465192	37.0	35.0	37.0	35.0	37.0
8	35.92410071177254	37.0	35.0	37.0	35.0	37.0
9	37.562182457125594	39.0	37.0	39.0	35.0	39.0
10	37.1454002816458	39.0	37.0	39.0	33.0	39.0
11	36.9106493594898	39.0	37.0	39.0	33.0	39.0
12	36.43444715312257	38.0	35.0	39.0	32.0	39.0
13	36.23546780005701	39.0	35.0	39.0	32.0	39.0
14	37.23013992946092	39.0	35.0	41.0	32.0	41.0
15	37.45703199785575	39.0	35.0	41.0	32.0	41.0
16	37.534307181116965	39.0	35.0	41.0	33.0	41.0
17	37.47260292622327	39.0	35.0	41.0	32.0	41.0
18	37.4042629771918	39.0	35.0	41.0	32.0	41.0
19	37.384727309857176	39.0	35.0	41.0	32.0	41.0
20	37.29045382412879	39.0	35.0	41.0	32.0	41.0
21	37.18617638174493	39.0	35.0	41.0	32.0	41.0
22	37.13424804400822	39.0	35.0	41.0	32.0	41.0
23	37.063950188685666	39.0	35.0	41.0	32.0	41.0
24	37.0194186694576	39.0	35.0	41.0	32.0	41.0
25	36.92881423715257	39.0	35.0	41.0	31.0	41.0
26	36.94109305798415	39.0	35.0	41.0	32.0	41.0
27	36.905746510272415	39.0	35.0	41.0	31.0	41.0
28	36.860373031776625	39.0	35.0	41.0	31.0	41.0
29	36.839087501648606	39.0	35.0	41.0	31.0	41.0
30	36.75749701123605	38.0	35.0	41.0	31.0	41.0
31	36.61442562551319	38.0	35.0	41.0	31.0	41.0
32	36.513718107442344	38.0	35.0	41.0	30.0	41.0
33	36.43963973162814	38.0	35.0	41.0	30.0	41.0
34	36.367038932639005	38.0	35.0	41.0	30.0	41.0
35	36.284857071138966	38.0	35.0	41.0	30.0	41.0
36	36.21195122677592	38.0	35.0	41.0	30.0	41.0
37	36.14139214710249	38.0	35.0	41.0	30.0	41.0
38	36.030182474143466	38.0	35.0	41.0	30.0	41.0
39	35.982005726514274	38.0	35.0	40.0	29.0	41.0
40	35.888915833854504	38.0	35.0	40.0	29.0	41.0
41	35.83619488868184	38.0	35.0	40.0	29.0	41.0
42	35.81205120677992	38.0	35.0	40.0	29.0	41.0
43	35.75966636459942	38.0	35.0	40.0	29.0	41.0
44	35.65751190187495	38.0	35.0	40.0	29.0	41.0
45	35.599669427816565	38.0	34.0	40.0	28.0	41.0
46	35.57380226082443	38.0	34.0	40.0	29.0	41.0
47	35.4933170812646	38.0	34.0	40.0	28.0	41.0
48	35.4171506124307	38.0	34.0	40.0	28.0	41.0
49	35.35130761081826	37.0	34.0	40.0	28.0	41.0
50	35.248885967487354	37.0	34.0	40.0	28.0	41.0
51	34.805053882840454	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	8.0
13	5.0
14	19.0
15	52.0
16	114.0
17	324.0
18	692.0
19	1345.0
20	2393.0
21	3911.0
22	6138.0
23	9088.0
24	13445.0
25	19320.0
26	25638.0
27	31048.0
28	36645.0
29	43464.0
30	53239.0
31	65905.0
32	83043.0
33	108527.0
34	179756.0
35	257349.0
36	172605.0
37	225735.0
38	345024.0
39	665416.0
40	218.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.46313715980208	23.83999795785524	29.950520534191032	12.746344348151647
2	30.256714614523904	25.77288797559637	30.985590116019353	12.984807293860376
3	30.060966530098238	24.921696511761475	30.849915123358308	14.16742183478198
4	26.823996902747112	27.25076261343476	30.48794496419865	15.437295519619482
5	24.747093134564576	31.842822924776748	28.2787697779593	15.131314162699375
6	22.498606661646395	41.255876484277614	25.314001029581316	10.931515824494674
7	87.60341548711534	3.9095159691465966	6.23024331303952	2.256825230698541
8	87.25429382208665	3.483388428697239	6.123541249196969	3.1387765000191448
9	81.24438941998834	6.817530110999077	8.438950507770786	3.499129961241794
10	40.81315651763264	36.10375797180989	12.992422792037337	10.090662718520125
11	32.90720579288398	21.75794628308381	27.54555471884347	17.78929320518875
12	31.821678217547976	20.95789352767744	28.70328062047165	18.517147634302926
13	25.2048313741507	28.887967087433573	28.500172305964337	17.407029232451382
14	18.470093215399473	33.09133917897272	30.35214233748995	18.086425268137862
15	16.928784455874784	27.746833612001005	38.196275638489325	17.12810629363489
16	21.396954651622867	25.51923657821627	35.27251996409228	17.811288806068575
17	21.9204669704357	25.36552264015282	27.576867605202366	25.137142784209114
18	23.10167753683306	26.35362289244279	31.24464468808366	19.300054882640495
19	24.35334209753794	28.689198330546656	27.088327015447973	19.86913255646743
20	25.66171872008577	28.038477410900796	27.355167264419457	18.944636604593974
21	24.0945853382515	27.3759716141878	29.925163903389535	18.604279144171166
22	22.78203933681349	23.030159925461717	30.217786229988047	23.97001450773675
23	18.541100290580182	28.208188149604123	30.196854246172045	23.053857313643654
24	20.0477351338243	26.87849664109731	33.282832795143094	19.79093542993529
25	20.354652473760567	28.30531765987228	29.51941526588299	21.82061460048416
26	19.363829361787214	31.76466834292716	27.04157040932239	21.829931885963234
27	19.049849604547177	30.1977051398231	30.270031100162946	20.48241415546678
28	17.1058979693423	28.02018319740307	34.32551787514838	20.54840095810625
29	18.40172391053704	27.11870391879071	33.25398750037227	21.225584670299984
30	21.46643011823167	27.295009083289724	31.693150731555818	19.545410066922784
31	24.36563751079571	27.797589418286556	27.507221959863347	20.329551111054382
32	24.297948920854125	28.107740154096838	28.284045318595858	19.31026560645318
33	23.523337885614367	27.320450803456332	27.9377741472982	21.218437163631105
34	18.9980301811978	28.969227431109523	29.966644968878565	22.06609741881411
35	19.376465132505412	27.57414474551898	30.483009781022517	22.566380340953085
36	24.135130420724366	26.69138512722987	29.336345496858073	19.837138955187687
37	19.67155505069199	30.453143413870414	29.146298399894487	20.729003135543103
38	19.90150905989015	29.945244993554482	27.093432377354315	23.059813569201054
39	19.473296829995704	28.09089245980591	29.172718647759808	23.263092062438577
40	21.672431471152578	26.293975247503692	28.657119639901808	23.376473641441926
41	19.071164490506153	25.64576446412845	28.728296893812725	26.554774151552667
42	21.529311159044788	26.169532051036605	27.870085557356617	24.431071232561997
43	21.006564644517905	26.536309759324734	28.660055222997954	23.79707037315941
44	20.390092194327092	27.81439456789493	29.271124498504552	22.52438873927342
45	19.600292707415964	29.51682004024727	27.6818678817428	23.20101937059397
46	22.21844992703587	28.023927129467722	28.462307538492304	21.295315405004107
47	20.26250069135109	27.76874412351572	29.25993524699316	22.70881993814003
48	20.93904623330653	26.93720830302025	30.072751407165377	22.050994056507847
49	21.306249388420188	25.128208400873014	30.30606644628521	23.259475764421584
50	19.646453687985808	27.048505192578503	30.849021685024695	22.45601943441099
51	18.62567911949525	28.54195118423124	28.462903164048043	24.36946653222547
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2671.0
1	3430.0
2	4189.0
3	9606.0
4	15023.0
5	10813.5
6	6604.0
7	6831.5
8	7059.0
9	7760.0
10	8461.0
11	8947.0
12	9433.0
13	8956.5
14	8480.0
15	8113.0
16	7746.0
17	7696.0
18	7646.0
19	8061.5
20	8477.0
21	8631.5
22	8786.0
23	10210.0
24	11634.0
25	14325.0
26	21194.5
27	25373.0
28	30163.5
29	34954.0
30	39372.0
31	43790.0
32	55161.5
33	66533.0
34	70171.5
35	73810.0
36	80634.0
37	87458.0
38	94327.5
39	101197.0
40	123744.0
41	146291.0
42	170699.0
43	195107.0
44	210826.5
45	226546.0
46	232836.0
47	239126.0
48	225741.5
49	212357.0
50	185932.0
51	159507.0
52	154382.0
53	149257.0
54	133119.0
55	116981.0
56	105458.5
57	93936.0
58	86669.5
59	79403.0
60	67972.5
61	56542.0
62	50434.5
63	44327.0
64	36589.5
65	28852.0
66	24085.0
67	19318.0
68	15034.0
69	10750.0
70	8971.0
71	7192.0
72	5855.5
73	4519.0
74	3353.5
75	1487.5
76	787.0
77	742.0
78	697.0
79	458.5
80	220.0
81	176.5
82	133.0
83	91.5
84	50.0
85	30.0
86	10.0
87	12.0
88	14.0
89	11.0
90	8.0
91	6.5
92	5.0
93	3.5
94	2.0
95	3.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2350470.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.38911136172026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.78585953516938	12.830705283773092
2	10.798200352685779	3.7554221687804006
3	4.167357568314505	2.17399934518586
4	2.158046224160799	1.5010602446268824
5	1.2641732548101219	1.099142475420079
6	0.8475109895857622	0.8842477786913135
7	0.5696625371119404	0.6934147707507743
8	0.4588087290693562	0.6382620866813101
9	0.36231230235676687	0.5670260076062773
>10	2.797461629685732	10.77216184600881
>50	1.1260287592175207	14.445518168018587
>100	1.651441929630236	47.99334214644986
>500	0.009170546480828108	1.0605029984855765
>1k	0.003717789113849234	1.2059633585558367
>5k	2.478526075899489E-4	0.37923132096539813
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8799	0.37435066178253706	No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	3818	0.1624355979867856	TruSeq Adapter, Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	2937	0.12495373265772378	Illumina PCR Primer Index 2 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2659	0.11312631090803116	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	2466	0.1049151871753309	RNA PCR Primer, Index 10 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.254468255285113E-5	0.0	0.0	0.17392266227605543	0.0
2	4.254468255285113E-5	0.0	0.0	0.5200236548434994	0.0
3	4.254468255285113E-5	0.0	0.0	0.7498074853114484	0.0
4	4.254468255285113E-5	0.0	0.0	1.1235625215382457	0.0
5	4.254468255285113E-5	0.0	0.0	1.7960237739686105	0.0
6	4.254468255285113E-5	0.0	0.0	2.4299395440060922	0.0
7	4.254468255285113E-5	0.0	0.0	2.814713653014078	0.0
8	4.254468255285113E-5	0.0	0.0	3.7971129178419636	0.0
9	4.254468255285113E-5	0.0	0.0	4.085693499597952	0.0
10	4.254468255285113E-5	0.0	0.0	4.639284908975652	0.0
11	4.254468255285113E-5	0.0	0.0	5.391942888018141	0.0
12	4.254468255285113E-5	0.0	0.0	6.046663007823967	0.0
13	4.254468255285113E-5	0.0	0.0	6.290912030359886	0.0
14	4.254468255285113E-5	0.0	0.0	6.393614894042468	0.0
15	4.254468255285113E-5	0.0	0.0	6.568984075525321	0.0
16	4.254468255285113E-5	0.0	0.0	6.9281462856364895	0.0
17	4.254468255285113E-5	0.0	0.0	7.358826107119001	0.0
18	4.254468255285113E-5	0.0	0.0	7.862384969814547	0.0
19	4.254468255285113E-5	0.0	0.0	8.161388998795985	0.0
20	4.254468255285113E-5	0.0	0.0	8.476389828417295	0.0
21	4.254468255285113E-5	0.0	0.0	8.842997357975213	0.0
22	4.254468255285113E-5	0.0	0.0	9.248192914608568	0.0
23	4.254468255285113E-5	0.0	0.0	9.650325254098117	0.0
24	4.254468255285113E-5	0.0	0.0	9.948733657523814	0.0
25	8.934383336098738E-4	0.0	0.0	10.201959608078385	0.0
26	8.934383336098738E-4	0.0	0.0	10.438678221802448	0.0
27	8.934383336098738E-4	0.0	0.0	10.708581687917736	0.0
28	8.934383336098738E-4	0.0	0.0	10.974358319825397	0.0
29	8.934383336098738E-4	0.0	0.0	11.285147225873974	0.0
30	8.934383336098738E-4	0.0	0.0	11.616442668913026	0.0
31	8.934383336098738E-4	0.0	0.0	11.91927571932422	0.0
32	8.934383336098738E-4	0.0	0.0	12.196199058060728	0.0
33	9.35983016162725E-4	0.0	0.0	12.479929546005692	0.0
34	9.35983016162725E-4	0.0	0.0	12.798206316183572	0.0
35	9.35983016162725E-4	0.0	0.0	13.137670338272772	0.0
36	9.35983016162725E-4	0.0	0.0	13.434632222491672	0.0
37	9.35983016162725E-4	0.0	0.0	13.741039026237306	0.0
38	9.35983016162725E-4	0.0	0.0	14.081609210072878	0.0
39	9.35983016162725E-4	0.0	0.0	14.556833314188227	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAATCG	25	3.8920538E-5	45.000004	18
CGCGTAC	25	3.8920538E-5	45.000004	10
CAATCGA	35	1.2126475E-7	45.000004	22
TATCGTG	45	3.8562575E-10	45.000004	1
GCGTACT	25	3.8920538E-5	45.000004	11
AGTCGAC	50	2.1827873E-11	45.000004	31
CGCTAGC	20	7.0347934E-4	45.0	42
TGTTACG	175	0.0	45.0	1
CGTACAC	20	7.0347934E-4	45.0	44
CCGGTTA	20	7.0347934E-4	45.0	32
TTCGCTA	20	7.0347934E-4	45.0	12
CCCGAAT	20	7.0347934E-4	45.0	34
CGACATA	30	2.1664746E-6	44.999996	10
GTTACGA	30	2.1664746E-6	44.999996	41
CTCGACC	30	2.1664746E-6	44.999996	17
CACGACG	890	0.0	43.98876	26
CGACGGT	920	0.0	42.798912	28
CGGTCTA	935	0.0	42.352943	31
TAGGGTA	1245	0.0	41.92771	5
TCCAACG	70	0.0	41.785717	10
>>END_MODULE