FastQCFastQC Report
Thu 26 May 2016
SRR1547533_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547533_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences358910
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80762.2501462762252378No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT4200.11702098019001979No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCT3770.10504026078961298No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAG253.8854254E-545.00000440
TTCGTGG253.8854254E-545.0000042
CGACAGG302.1613141E-645.0000042
ATCGTGA253.8854254E-545.00000430
CTACGGG253.8854254E-545.0000043
CGCAGTG253.8854254E-545.00000442
TCGTAAG302.1613141E-645.0000041
ACGTAAG253.8854254E-545.0000041
GTACGAG302.1613141E-645.0000041
TCACGAC453.8380676E-1045.025
TCGTTCA207.0267974E-445.016
GCGACGG453.8380676E-1045.037
GCGACCT207.0267974E-445.011
ATGATCG207.0267974E-445.027
CACGGCC207.0267974E-445.044
CGACGGT453.8380676E-1045.028
GCGCGAC207.0267974E-445.09
CGCATGG351.2088094E-745.02
ATCAGCG207.0267974E-445.01
CCAATCG453.8380676E-1045.024