##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547533_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 358910 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.429015630659496 31.0 31.0 33.0 30.0 34.0 2 31.843927446992282 31.0 31.0 34.0 30.0 34.0 3 31.830935331977376 31.0 31.0 34.0 30.0 34.0 4 35.67196790281686 37.0 35.0 37.0 33.0 37.0 5 35.6172745256471 37.0 35.0 37.0 33.0 37.0 6 35.67835947730629 37.0 35.0 37.0 33.0 37.0 7 36.01188877434454 37.0 35.0 37.0 35.0 37.0 8 35.986015992867294 37.0 35.0 37.0 35.0 37.0 9 37.6772505642083 39.0 38.0 39.0 35.0 39.0 10 37.251542169346074 39.0 37.0 39.0 34.0 39.0 11 36.93253183249283 39.0 37.0 39.0 33.0 39.0 12 36.6088267253629 39.0 35.0 39.0 32.0 39.0 13 36.489222924967265 39.0 35.0 39.0 32.0 39.0 14 37.56407177286785 40.0 36.0 41.0 32.0 41.0 15 37.781786520297565 40.0 36.0 41.0 33.0 41.0 16 37.83850268869633 40.0 36.0 41.0 33.0 41.0 17 37.78924521467778 40.0 36.0 41.0 33.0 41.0 18 37.635195452899055 39.0 36.0 41.0 33.0 41.0 19 37.53200523808197 39.0 36.0 41.0 33.0 41.0 20 37.33554930205344 39.0 35.0 41.0 32.0 41.0 21 37.22289710512385 39.0 35.0 41.0 32.0 41.0 22 37.17963277701931 39.0 35.0 41.0 32.0 41.0 23 37.1220946755454 39.0 35.0 41.0 32.0 41.0 24 37.0099161349642 39.0 35.0 41.0 32.0 41.0 25 36.905689448608285 38.0 35.0 41.0 32.0 41.0 26 36.86633975091249 38.0 35.0 41.0 32.0 41.0 27 36.81173274637096 38.0 35.0 41.0 32.0 41.0 28 36.724223342899336 38.0 35.0 41.0 31.0 41.0 29 36.658343317266166 38.0 35.0 41.0 31.0 41.0 30 36.50394249254688 38.0 35.0 41.0 31.0 41.0 31 36.28884957231618 38.0 35.0 40.0 30.0 41.0 32 36.014973113036696 38.0 35.0 41.0 30.0 41.0 33 35.75617285670502 38.0 35.0 41.0 29.0 41.0 34 35.55354267086456 38.0 35.0 41.0 27.0 41.0 35 35.35280711041765 38.0 35.0 41.0 25.0 41.0 36 35.23544621214232 38.0 35.0 41.0 25.0 41.0 37 35.18202056225795 38.0 34.0 41.0 24.0 41.0 38 35.03542670864562 38.0 34.0 40.0 24.0 41.0 39 34.96094285475468 38.0 34.0 40.0 24.0 41.0 40 34.859552534061464 38.0 34.0 40.0 23.0 41.0 41 34.792892368560366 38.0 34.0 40.0 23.0 41.0 42 34.787765735142514 38.0 34.0 40.0 23.0 41.0 43 34.746067259201475 38.0 34.0 40.0 23.0 41.0 44 34.639115655735424 38.0 34.0 40.0 23.0 41.0 45 34.59750633863643 38.0 34.0 40.0 23.0 41.0 46 34.5574573012733 37.0 34.0 40.0 23.0 41.0 47 34.51281658354462 37.0 34.0 40.0 23.0 41.0 48 34.400236828174194 37.0 34.0 40.0 23.0 41.0 49 34.33621799336881 37.0 34.0 40.0 23.0 41.0 50 34.226839597670725 37.0 33.0 40.0 23.0 41.0 51 33.868393190493435 36.0 33.0 40.0 22.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 0.0 12 2.0 13 2.0 14 4.0 15 9.0 16 32.0 17 55.0 18 154.0 19 302.0 20 544.0 21 838.0 22 1348.0 23 2001.0 24 2985.0 25 4834.0 26 6621.0 27 7334.0 28 7223.0 29 7538.0 30 8339.0 31 9809.0 32 11899.0 33 15418.0 34 24750.0 35 35356.0 36 29258.0 37 33373.0 38 49990.0 39 98862.0 40 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.051823576941295 23.401688445571313 28.92897940987991 17.61750856760748 2 34.42506477947118 24.381599843972026 29.057981109470344 12.135354267086457 3 27.434175698643116 23.806246691371097 35.83878966871918 12.920787941266612 4 25.162018333286895 25.756874982586165 35.544844111337106 13.536262572789838 5 22.718508818366722 31.009445264829623 33.31447995319161 12.957565963612048 6 21.401186927084783 38.390682901005825 30.167172828842887 10.040957343066507 7 84.25566297957705 3.1729402914379654 11.023933576662673 1.5474631523223092 8 85.57382073500321 2.1785405812042016 10.657267838733944 1.5903708450586498 9 81.12535175949402 4.913488061073807 11.823019698531665 2.1381404809005042 10 40.18305424758296 35.055584965590256 16.48435540943412 8.277005377392662 11 27.507731743333984 24.298292050931988 32.70151291410103 15.492463291633 12 27.789417959934244 23.047839291187206 32.45716196260901 16.70558078626954 13 22.734390237106798 27.57209328243849 32.51762280237385 17.175893678080854 14 15.98952383605918 32.15903708450586 34.10687916190688 17.744559917528072 15 15.056142208353068 30.027583516759076 39.500710484522585 15.415563790365272 16 20.26775514753002 28.22546042183277 36.8075562118637 14.699228218773507 17 20.131230670641663 27.0680672034772 32.01387534479396 20.786826781087182 18 20.307040762308098 29.449165528962695 33.579448886907585 16.66434482182163 19 22.298347775208267 30.671198907804186 30.62801259368644 16.40244072330111 20 23.199130701290017 30.594856649299267 30.524922682566658 15.681089966844056 21 22.502020005015186 28.438327157226045 33.21807695522555 15.841575882533226 22 20.29088072218662 25.9154105486055 34.35596667688278 19.437742052325095 23 17.921484494720126 29.648658438048535 33.884817920927254 18.545039146304088 24 17.650943133376053 29.798835362625724 35.50249366136357 17.047727842634643 25 18.542531553871445 30.39731408988326 33.08684628458388 17.97330807166142 26 17.262266306316345 34.52704020506533 30.018110389791314 18.192583098827004 27 17.031567802513166 32.7811986291828 33.90905798110947 16.27817558719456 28 16.22523752472765 30.978796912875094 35.72121144576635 17.07475411663091 29 17.11821905213006 30.341589813602294 34.67777437240534 17.862416761862303 30 18.768772115572148 30.343818784653536 33.05480482572233 17.83260427405199 31 21.49006714775292 30.213423978156083 30.605722883174053 17.690785990916943 32 21.853389429104787 31.904934384664678 29.97743166810621 16.264244518124322 33 21.001922487531694 31.488116798083084 30.252988214315568 17.256972500069658 34 18.435819564793402 32.24290212030871 30.90774846061687 18.41352985428102 35 18.388453929954586 31.86230531330974 30.413195508623332 19.33604524811234 36 21.447716697779388 31.20057953247332 29.28672926360369 18.064974506143603 37 19.36557911454125 33.13393329804129 29.693795101836113 17.806692485581344 38 19.96043576384052 32.55635117438912 28.133793987350593 19.34941907441977 39 19.198406285698365 32.153464656877766 28.77072246524198 19.877406592181885 40 20.628848457830653 31.025048062188294 29.452787606920953 18.8933158730601 41 17.554818756791395 30.699618288707477 30.87264216655986 20.872920787941265 42 19.786854643225322 31.68705246440612 29.078877713075702 19.447215179292858 43 20.217603298877158 31.347691621855063 28.88997241648324 19.544732662784543 44 19.054080410130673 33.14507815329748 29.196177314647127 18.604664121924717 45 18.078348332451032 33.488061073806804 28.188961020868742 20.24462957287342 46 20.07606363712351 32.7290964308601 28.357805578000058 18.83703435401633 47 19.402914379649495 32.42512050374746 29.40765094313338 18.764314173469675 48 19.684043353486945 31.90437714190187 29.065225265386868 19.34635423922432 49 18.96993675294642 30.50346883619849 30.246579922543255 20.280014488311835 50 18.034326154189074 32.20668134072609 30.24853027221309 19.51046223287175 51 17.796940737232177 33.264049483157336 28.837870218160543 20.101139561449948 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 292.0 1 402.5 2 513.0 3 5481.5 4 10450.0 5 6749.0 6 3048.0 7 3051.0 8 3054.0 9 3648.5 10 4243.0 11 4619.0 12 4995.0 13 5202.5 14 5410.0 15 5124.5 16 4839.0 17 4430.5 18 4022.0 19 3681.5 20 3341.0 21 3290.0 22 3239.0 23 3236.5 24 3234.0 25 3725.0 26 4541.0 27 4866.0 28 5705.0 29 6544.0 30 7525.5 31 8507.0 32 9996.5 33 11486.0 34 12144.5 35 12803.0 36 13451.5 37 14100.0 38 15858.5 39 17617.0 40 19929.5 41 22242.0 42 23549.5 43 24857.0 44 25577.0 45 26297.0 46 26226.5 47 26156.0 48 26503.5 49 26851.0 50 25780.0 51 24709.0 52 22610.0 53 20511.0 54 18130.5 55 15750.0 56 14420.0 57 13090.0 58 11672.5 59 10255.0 60 8830.0 61 7405.0 62 5945.0 63 4485.0 64 3542.5 65 2600.0 66 1862.5 67 1125.0 68 957.0 69 789.0 70 606.5 71 424.0 72 314.0 73 204.0 74 195.0 75 128.5 76 71.0 77 54.5 78 38.0 79 29.0 80 20.0 81 17.5 82 15.0 83 10.0 84 5.0 85 4.0 86 3.0 87 2.0 88 1.0 89 1.0 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 358910.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.09021167313357 #Duplication Level Percentage of deduplicated Percentage of total 1 71.82572531785578 25.20379904979908 2 8.928380973428943 6.26597556512 3 4.130297590773846 4.347990501998636 4 2.5090433162942936 3.521714442633111 5 1.8498849101938948 3.2456426534819727 6 1.4269071963643263 3.004228533500507 7 1.1229926456420303 2.758423475006578 8 0.9432099639553689 2.647794982992206 9 0.7866576607706794 2.484358544766172 >10 6.385057843224505 41.477689483200834 >50 0.07347406519706282 1.581682228631968 >100 0.01756988515581937 1.1974713258228915 >500 0.0 0.0 >1k 0.0 0.0 >5k 7.98631143446335E-4 2.2632292130460265 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8076 2.2501462762252378 No Hit CGTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT 420 0.11702098019001979 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCT 377 0.10504026078961298 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.06659051015574935 0.0 2 0.0 0.0 0.0 0.173581120615196 0.0 3 0.0 0.0 0.0 0.2783427600234042 0.0 4 0.0 0.0 0.0 0.3825471566688028 0.0 5 0.0 0.0 0.0 0.643336769663704 0.0 6 0.0 0.0 0.0 1.0802150957064445 0.0 7 0.0 0.0 0.0 1.4624836309938425 0.0 8 0.0 0.0 0.0 2.016104315845198 0.0 9 0.0 0.0 0.0 2.372461062661949 0.0 10 0.0 0.0 0.0 2.7402412861162966 0.0 11 0.0 0.0 0.0 3.125017413836338 0.0 12 0.0 0.0 0.0 3.3846925413056197 0.0 13 0.0 0.0 0.0 3.491961773146471 0.0 14 0.0 0.0 0.0 3.568304031651389 0.0 15 0.0 0.0 0.0 3.627929007272018 0.0 16 0.0 0.0 0.0 3.7340837535872504 0.0 17 0.0 0.0 0.0 3.871444094619821 0.0 18 0.0 0.0 0.0 4.047254186286255 0.0 19 0.0 0.0 0.0 4.144771669777938 0.0 20 0.0 0.0 0.0 4.268479563121674 0.0 21 0.0 0.0 0.0 4.3871722716001225 0.0 22 0.0 0.0 0.0 4.535120225126076 0.0 23 0.0 0.0 0.0 4.706472374690033 0.0 24 0.0 0.0 0.0 4.831294753559388 0.0 25 0.0 0.0 0.0 4.936335014349001 0.0 26 0.0 0.0 0.0 5.042768382045638 0.0 27 0.0 0.0 0.0 5.16396868295673 0.0 28 0.0 0.0 0.0 5.277924827951297 0.0 29 0.0 0.0 0.0 5.422250703518988 0.0 30 0.0 0.0 0.0 5.582736619208158 0.0 31 0.0 0.0 0.0 5.735142514836588 0.0 32 0.0 0.0 0.0 5.880025633167089 0.0 33 0.0 0.0 0.0 6.0246301301161855 0.0 34 0.0 0.0 0.0 6.176200161600401 0.0 35 0.0 0.0 0.0 6.340586776629238 0.0 36 0.0 0.0 0.0 6.494385779164693 0.0 37 0.0 0.0 0.0 6.652085481039815 0.0 38 0.0 0.0 0.0 6.838483185199633 0.0 39 0.0 0.0 0.0 7.147474297177565 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGCAG 25 3.8854254E-5 45.000004 40 TTCGTGG 25 3.8854254E-5 45.000004 2 CGACAGG 30 2.1613141E-6 45.000004 2 ATCGTGA 25 3.8854254E-5 45.000004 30 CTACGGG 25 3.8854254E-5 45.000004 3 CGCAGTG 25 3.8854254E-5 45.000004 42 TCGTAAG 30 2.1613141E-6 45.000004 1 ACGTAAG 25 3.8854254E-5 45.000004 1 GTACGAG 30 2.1613141E-6 45.000004 1 TCACGAC 45 3.8380676E-10 45.0 25 TCGTTCA 20 7.0267974E-4 45.0 16 GCGACGG 45 3.8380676E-10 45.0 37 GCGACCT 20 7.0267974E-4 45.0 11 ATGATCG 20 7.0267974E-4 45.0 27 CACGGCC 20 7.0267974E-4 45.0 44 CGACGGT 45 3.8380676E-10 45.0 28 GCGCGAC 20 7.0267974E-4 45.0 9 CGCATGG 35 1.2088094E-7 45.0 2 ATCAGCG 20 7.0267974E-4 45.0 1 CCAATCG 45 3.8380676E-10 45.0 24 >>END_MODULE