##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547532_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3027013
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.364664439828967	31.0	31.0	33.0	30.0	34.0
2	31.772313498488444	31.0	31.0	34.0	30.0	34.0
3	31.935974176523192	31.0	31.0	34.0	30.0	34.0
4	35.66960762969964	37.0	35.0	37.0	33.0	37.0
5	35.57036920555016	37.0	35.0	37.0	33.0	37.0
6	35.621506746089295	37.0	35.0	37.0	33.0	37.0
7	35.92267657918879	37.0	35.0	37.0	35.0	37.0
8	35.84728443518412	37.0	35.0	37.0	35.0	37.0
9	37.45024847927643	39.0	37.0	39.0	35.0	39.0
10	37.07849454230953	39.0	37.0	39.0	33.0	39.0
11	36.87018952346752	39.0	37.0	39.0	32.0	39.0
12	36.39047272013698	38.0	35.0	39.0	32.0	39.0
13	36.19426576628511	39.0	35.0	39.0	32.0	39.0
14	37.31552656034183	39.0	35.0	41.0	32.0	41.0
15	37.47507889791025	39.0	35.0	41.0	32.0	41.0
16	37.56115979680299	39.0	35.0	41.0	33.0	41.0
17	37.45530957415776	39.0	35.0	41.0	32.0	41.0
18	37.384711925584725	39.0	35.0	41.0	32.0	41.0
19	37.36824057247194	39.0	35.0	41.0	32.0	41.0
20	37.26137086295962	39.0	35.0	41.0	32.0	41.0
21	37.14659633110264	39.0	35.0	41.0	32.0	41.0
22	37.04696312833807	39.0	35.0	41.0	31.0	41.0
23	36.96631629926928	39.0	35.0	41.0	31.0	41.0
24	36.89106059339686	39.0	35.0	41.0	31.0	41.0
25	36.80355122359897	38.0	35.0	41.0	31.0	41.0
26	36.78723249619344	39.0	35.0	41.0	31.0	41.0
27	36.762486979738775	39.0	35.0	41.0	31.0	41.0
28	36.68684112027269	38.0	35.0	41.0	31.0	41.0
29	36.65362322527191	38.0	35.0	41.0	31.0	41.0
30	36.5569391343876	38.0	35.0	41.0	31.0	41.0
31	36.46409215949849	38.0	35.0	41.0	30.0	41.0
32	36.32607854673898	38.0	35.0	41.0	30.0	41.0
33	36.22230000333662	38.0	35.0	41.0	30.0	41.0
34	36.139373699419195	38.0	35.0	41.0	30.0	41.0
35	36.06161585695205	38.0	35.0	41.0	30.0	41.0
36	36.001797811902364	38.0	35.0	41.0	29.0	41.0
37	35.95794666227069	38.0	35.0	41.0	29.0	41.0
38	35.83158909459589	38.0	35.0	41.0	29.0	41.0
39	35.791617016511	38.0	35.0	40.0	29.0	41.0
40	35.68232974222443	38.0	35.0	40.0	28.0	41.0
41	35.64740488395656	38.0	35.0	40.0	28.0	41.0
42	35.631564185551895	38.0	35.0	40.0	28.0	41.0
43	35.579105540676565	38.0	34.0	40.0	28.0	41.0
44	35.46991770435079	38.0	34.0	40.0	28.0	41.0
45	35.395362689225315	38.0	34.0	40.0	27.0	41.0
46	35.323365641310424	38.0	34.0	40.0	27.0	41.0
47	35.251383789894525	37.0	34.0	40.0	27.0	41.0
48	35.15184506971064	37.0	34.0	40.0	27.0	41.0
49	35.08856982114051	37.0	34.0	40.0	27.0	41.0
50	35.000030392997985	37.0	34.0	40.0	27.0	41.0
51	34.57518154035017	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	5.0
11	5.0
12	8.0
13	12.0
14	26.0
15	76.0
16	202.0
17	526.0
18	1118.0
19	2207.0
20	3994.0
21	6496.0
22	9959.0
23	14545.0
24	20276.0
25	28340.0
26	37197.0
27	44228.0
28	50432.0
29	58641.0
30	70184.0
31	85861.0
32	107043.0
33	137393.0
34	227614.0
35	331918.0
36	218147.0
37	288061.0
38	440931.0
39	841202.0
40	362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.2435840216081	23.803333517232993	27.543687456908838	12.409395004250065
2	31.410998234893604	26.04177781859543	28.615866532452948	13.931357414058018
3	30.856953703205107	25.727177253616023	29.407009484267167	14.008859558911707
4	28.080487265829383	26.975536609852686	29.530101126093612	15.413874998224323
5	24.59051216496262	32.144757885083415	27.665292484703567	15.599437465250396
6	22.58890199678693	41.86975741432231	24.889916230951105	10.651424357939659
7	86.40977095242076	4.820758946195474	6.6492281334767975	2.1202419679069764
8	86.1619028395319	5.009591964091334	6.192639410534411	2.6358657858423467
9	79.81967702153906	8.04340780829154	8.085726754394514	4.051188415774891
10	41.308676242883664	33.88614452597329	13.733902034778179	11.071277196364864
11	35.690299314869144	24.97006124519452	23.255962230753553	16.08367720918278
12	34.85564812572658	22.239514663465272	26.104281679662428	16.80055553114572
13	25.16193356288856	29.816588167939813	25.516903957796018	19.504574311375602
14	17.930349159385838	34.98362907592402	26.9767919728128	20.10922979187734
15	18.478017768671627	28.55290016924275	35.65138306310545	17.317698998980184
16	21.22940998271233	25.468010874086104	34.94071548420836	18.361863658993204
17	20.17639171024373	25.6242705267536	28.073087231538153	26.126250531464517
18	21.962938381830536	28.330205387291034	29.35771997014879	20.349136260729637
19	26.48336825775112	28.765485975778766	24.594476469047212	20.156669297422905
20	29.44685074031727	26.516073766448972	25.92730853815296	18.1097669550808
21	24.85536071368045	28.67321679820998	26.766518676992796	19.70490381111677
22	23.483645428678372	25.544455871183903	27.304078310862888	23.66782038927484
23	22.99425208943602	29.103475934857233	26.7004139063823	21.201858069324448
24	22.070172807318635	26.403784853253025	31.47948158795486	20.046560751473482
25	21.498057656177888	28.21880844251412	28.41920401399003	21.86392988731796
26	20.057594731175584	32.88720596839194	25.729820123005748	21.325379177426722
27	21.06165384819953	31.597518742073454	27.225023480242733	20.11580392948428
28	18.470155232237193	31.049222451307607	31.458173453500198	19.022448862955
29	20.566578339769272	27.67933272833648	30.54780405634201	21.206284875552235
30	20.765189974407114	29.3663092956654	28.60826828295749	21.26023244697
31	25.744223761179754	28.66618015845984	24.575315665971704	21.014280414388704
32	26.548250701268877	29.433504249899155	25.11201636729013	18.906228681541837
33	24.97303447325796	30.0885724640099	24.38773801103596	20.550655051696175
34	21.164593610929323	29.856825854398377	27.463707622002282	21.514872912670015
35	22.406874367569614	27.497437242588653	27.450658454390513	22.64502993545122
36	23.334257236424158	30.687281488384755	25.758297040680038	20.220164234511053
37	21.961583911268303	30.945952329904102	26.748084662999467	20.34437909582813
38	21.467400371257078	31.38595704742596	25.090278766559642	22.056363814757322
39	22.58923235546065	28.98527360140178	25.72377455927675	22.70171948386082
40	22.95256082481311	28.383690456565596	27.60444041700515	21.05930830161615
41	19.181219241542735	27.185909013274802	28.57087168109288	25.062000064089585
42	21.96703482938461	28.537042952904397	25.281688582110483	24.214233635600507
43	22.84298085274163	27.78210731172942	25.4639474623994	23.910964373129552
44	21.58768396435694	30.01163192890153	26.395492850542762	22.005191256198767
45	20.994789252639485	31.40049282906945	24.769500494381756	22.835217423909313
46	21.383324088796446	32.46272810853472	24.45447046312652	21.699477339542312
47	22.41225921395118	28.737702811319277	26.71759255741551	22.13244541731403
48	22.01939667916854	26.86308251731988	28.936512661161352	22.18100814235023
49	20.413655309706304	27.970808186155793	28.76290917812378	22.852627326014126
50	19.505102885253546	29.210247858202127	28.54553317081889	22.739116085725435
51	19.8072158923665	29.17648520174839	26.49341116143208	24.52288774445303
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2363.0
1	4319.5
2	6276.0
3	13097.5
4	19919.0
5	14928.5
6	9938.0
7	9871.5
8	9805.0
9	9934.5
10	10064.0
11	10195.0
12	10326.0
13	10393.0
14	10460.0
15	10344.0
16	10228.0
17	9625.5
18	9023.0
19	10466.5
20	11910.0
21	11479.5
22	11049.0
23	13047.5
24	15046.0
25	18899.0
26	26973.5
27	31195.0
28	41907.5
29	52620.0
30	60406.0
31	68192.0
32	76654.5
33	85117.0
34	88209.5
35	91302.0
36	94622.0
37	97942.0
38	113032.0
39	128122.0
40	154712.5
41	181303.0
42	203064.5
43	224826.0
44	225792.5
45	226759.0
46	228045.0
47	229331.0
48	232954.5
49	236578.0
50	233015.5
51	229453.0
52	216298.5
53	203144.0
54	185582.5
55	168021.0
56	156543.5
57	145066.0
58	141149.5
59	137233.0
60	127371.5
61	117510.0
62	100014.0
63	82518.0
64	65771.5
65	49025.0
66	38409.0
67	27793.0
68	23531.5
69	19270.0
70	17047.0
71	14824.0
72	12139.0
73	9454.0
74	7368.0
75	4338.0
76	3394.0
77	2280.5
78	1167.0
79	988.5
80	810.0
81	583.0
82	356.0
83	249.5
84	143.0
85	93.5
86	44.0
87	39.5
88	35.0
89	24.5
90	14.0
91	10.0
92	6.0
93	4.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3027013.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.92753167299491
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.2662666202534	12.306609088481554
2	9.56261698189311	3.0461776971164296
3	3.4745329223645585	1.6602219950947523
4	1.6786893104859129	1.0694950864752943
5	0.9851648675517373	0.7845622315526065
6	0.670383133628041	0.6406529156341322
7	0.47346613546455596	0.527880280809155
8	0.3539608956493514	0.45101787011653527
9	0.26138295941479	0.3746866828374189
>10	2.3817300922532594	8.766773906696336
>50	1.044974644717943	12.265291066082744
>100	1.8249280880258598	54.07541429622127
>500	0.015795683868377812	1.6498691161786923
>1k	0.005686446192616013	1.8008879589128433
>5k	2.1060911824503752E-4	0.22894349215028414
>10k+	2.1060911824503752E-4	0.3515163156399747
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10479	0.346182854186619	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	6825	0.22546979481092416	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4960	0.16385790216295734	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	4559	0.15061051934696018	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3832	0.12659344376783319	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3334	0.1101415818167943	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.607173474312796E-5	0.0	0.0	0.06356100882288909	0.0
2	6.607173474312796E-5	0.0	0.0	0.1910464210097545	0.0
3	6.607173474312796E-5	0.0	0.0	0.26950660601721893	0.0
4	6.607173474312796E-5	0.0	0.0	0.42679037057323505	0.0
5	6.607173474312796E-5	0.0	0.0	0.7659035491423394	0.0
6	6.607173474312796E-5	0.0	0.0	1.0559254287972994	0.0
7	6.607173474312796E-5	0.0	0.0	1.249251324655692	0.0
8	6.607173474312796E-5	0.0	0.0	1.6929890951905393	0.0
9	6.607173474312796E-5	0.0	0.0	1.8228861256955289	0.0
10	6.607173474312796E-5	0.0	0.0	2.1650055681954457	0.0
11	6.607173474312796E-5	0.0	0.0	2.5649708144629706	0.0
12	6.607173474312796E-5	0.0	0.0	2.9523163593945583	0.0
13	6.607173474312796E-5	0.0	0.0	3.097277745420981	0.0
14	6.607173474312796E-5	0.0	0.0	3.1562798045465943	0.0
15	6.607173474312796E-5	0.0	0.0	3.2652651310053837	0.0
16	6.607173474312796E-5	0.0	0.0	3.4786437983583154	0.0
17	6.607173474312796E-5	0.0	0.0	3.7090359374076027	0.0
18	6.607173474312796E-5	0.0	0.0	3.9959854813970077	0.0
19	6.607173474312796E-5	0.0	0.0	4.177980074746953	0.0
20	6.607173474312796E-5	0.0	0.0	4.357496978044032	0.0
21	6.607173474312796E-5	0.0	0.0	4.581546230557979	0.0
22	6.607173474312796E-5	0.0	0.0	4.831958105234434	0.0
23	6.607173474312796E-5	0.0	0.0	5.096344151809061	0.0
24	6.607173474312796E-5	0.0	0.0	5.293733459354155	0.0
25	6.607173474312796E-5	0.0	0.0	5.4693190944340175	0.0
26	6.607173474312796E-5	0.0	0.0	5.635621650782471	0.0
27	6.607173474312796E-5	0.0	0.0	5.807639412186204	0.0
28	6.607173474312796E-5	0.0	0.0	5.996769752888408	0.0
29	6.607173474312796E-5	0.0	0.0	6.208992164883336	0.0
30	6.607173474312796E-5	0.0	0.0	6.4387235865851915	0.0
31	9.910760211469194E-5	0.0	0.0	6.669313940838708	0.0
32	9.910760211469194E-5	0.0	0.0	6.879587236658713	0.0
33	9.910760211469194E-5	0.0	0.0	7.08926588686603	0.0
34	9.910760211469194E-5	0.0	0.0	7.298118640389057	0.0
35	9.910760211469194E-5	0.0	0.0	7.53729832015918	0.0
36	9.910760211469194E-5	0.0	0.0	7.785199468915396	0.0
37	9.910760211469194E-5	0.0	0.0	8.032935438334754	0.0
38	9.910760211469194E-5	0.0	0.0	8.269868679123611	0.0
39	9.910760211469194E-5	0.0	0.0	8.509775147975908	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGA	30	2.166682E-6	45.000004	31
GTCGATA	50	2.1827873E-11	45.0	32
ACGCATC	35	1.2128112E-7	45.0	32
TCGAACG	35	1.2128112E-7	45.0	1
TACGTCG	65	0.0	44.999996	1
ATCGGTA	95	0.0	42.63158	27
CGACGTG	55	6.184564E-11	40.909092	14
CGTTTTT	6250	0.0	40.5	1
CGGTCTA	575	0.0	40.304348	31
CGCATCG	180	0.0	40.0	21
CCAGTCG	180	0.0	40.0	28
CGAACTA	40	3.4607183E-7	39.375	32
CGAATTA	40	3.4607183E-7	39.375	33
TCGAATC	80	0.0	39.375	17
GCGAGAC	690	0.0	39.130436	21
ATGGGAC	1885	0.0	38.793102	5
ACGGTCT	605	0.0	38.67769	30
TGTGCGA	35	6.251672E-6	38.571426	21
AACGGGA	1190	0.0	38.571426	4
CCGTTTA	35	6.251672E-6	38.571426	29
>>END_MODULE