FastQCFastQC Report
Thu 26 May 2016
SRR1547531_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547531_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4593193
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT886931.9309661057133893No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTT73050.15903969199639553No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT57750.1257295306337008No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC46040.10023528295022657No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT417500.044.0676651
CGACGGT5100.042.3529428
CGGTCTA5100.041.4705931
TACGGGA20500.040.9390264
GGCGATA9400.040.2127658
GCGCGAC6400.039.3759
TAGGGAC45550.038.232715
ACGGGAC16150.038.1733745
GCGATAC1950.038.0769239
ATTCGCG659.094947E-1238.076921
TAGGGAT74450.038.0188035
ACGGGAT21750.037.9655155
AGGGATC60050.037.7310566
ATAGGGA64350.037.6923074
ATTAGCG2750.037.6363641
CGGTCGA601.5643309E-1037.49999627
AGTACGG5600.037.3660742
TAAGGGA64550.037.0178154
AGGGCGA20100.036.94036
GGGCGAT37950.036.818187
Produced by FastQC (version 0.11.5)