FastQCFastQC Report
Thu 26 May 2016
SRR1547530_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547530_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2841092
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306311.0781417849193198No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGA207.0350413E-445.00000438
GTCGTAA253.892262E-545.020
CGTTTTT147350.043.4577521
ATTAGCG2550.043.2352941
CGGTCTA4300.042.90697531
CGACGGT4350.042.41379528
GCGATAC800.042.1875049
TATCGCG603.6379788E-1241.2500041
TCACGAC4600.041.0869625
AGTACGG3900.040.9615362
AGTTGCG1000.040.51
TAGTGCG2000.040.51
ACGGGAT13650.040.0549475
CCGCTCG451.9299478E-840.019
TACGGGA13700.039.7445264
ACGACGG4650.039.6774227
TATAGCG1250.039.6000021
GCGATAT2050.039.5121969
TGTTGCG1950.039.230771
CGTTAGG3850.039.155842
Produced by FastQC (version 0.11.5)