##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547530_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2841092
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.359103119504752	31.0	31.0	33.0	30.0	34.0
2	31.776807650016263	31.0	31.0	34.0	30.0	34.0
3	31.856186283302335	31.0	31.0	34.0	30.0	34.0
4	35.63816236855406	37.0	35.0	37.0	33.0	37.0
5	35.548661218996074	37.0	35.0	37.0	33.0	37.0
6	35.60176333606937	37.0	35.0	37.0	33.0	37.0
7	35.97940263814055	37.0	35.0	37.0	35.0	37.0
8	35.94346715981038	37.0	35.0	37.0	35.0	37.0
9	37.60929494715412	39.0	37.0	39.0	35.0	39.0
10	37.140600867553744	39.0	37.0	39.0	33.0	39.0
11	36.841659826573725	39.0	37.0	39.0	32.0	39.0
12	36.56099344899778	39.0	35.0	39.0	32.0	39.0
13	36.446133388147935	39.0	35.0	39.0	32.0	39.0
14	37.510817671515035	40.0	36.0	41.0	32.0	41.0
15	37.71490117180296	40.0	36.0	41.0	32.0	41.0
16	37.773536724611525	40.0	36.0	41.0	33.0	41.0
17	37.67958798940689	40.0	36.0	41.0	32.0	41.0
18	37.57404688056564	39.0	36.0	41.0	32.0	41.0
19	37.53692453465076	39.0	36.0	41.0	32.0	41.0
20	37.39873048813625	39.0	35.0	41.0	32.0	41.0
21	37.25607583281358	39.0	35.0	41.0	32.0	41.0
22	37.202573869483984	39.0	35.0	41.0	32.0	41.0
23	37.1296902740214	39.0	35.0	41.0	32.0	41.0
24	37.06607846560407	39.0	35.0	41.0	32.0	41.0
25	36.965503053051435	39.0	35.0	41.0	31.0	41.0
26	36.96998478049989	39.0	35.0	41.0	32.0	41.0
27	36.92270331266992	39.0	35.0	41.0	31.0	41.0
28	36.85407617915928	39.0	35.0	41.0	31.0	41.0
29	36.81483457769055	39.0	35.0	41.0	31.0	41.0
30	36.7143886927984	39.0	35.0	41.0	31.0	41.0
31	36.562616064527305	39.0	35.0	41.0	30.0	41.0
32	36.39406573247188	39.0	35.0	41.0	30.0	41.0
33	36.216832471458154	39.0	35.0	41.0	30.0	41.0
34	36.076703605515064	39.0	35.0	41.0	29.0	41.0
35	35.91341991037249	39.0	35.0	41.0	29.0	41.0
36	35.82857964472815	38.0	35.0	41.0	28.0	41.0
37	35.77888150049347	38.0	35.0	41.0	27.0	41.0
38	35.64900538243746	38.0	35.0	41.0	27.0	41.0
39	35.58099596915552	38.0	35.0	41.0	27.0	41.0
40	35.5156496164151	38.0	35.0	41.0	26.0	41.0
41	35.436770438972054	38.0	35.0	40.0	26.0	41.0
42	35.42688550740349	38.0	35.0	40.0	26.0	41.0
43	35.37639013449758	38.0	34.0	40.0	26.0	41.0
44	35.27112955159495	38.0	34.0	40.0	26.0	41.0
45	35.202038511952445	38.0	34.0	40.0	25.0	41.0
46	35.17256815337201	38.0	34.0	40.0	26.0	41.0
47	35.126959633830936	38.0	34.0	40.0	25.0	41.0
48	35.0207944691689	38.0	34.0	40.0	25.0	41.0
49	34.954459412085214	38.0	34.0	40.0	24.0	41.0
50	34.85008475614306	38.0	34.0	40.0	24.0	41.0
51	34.418615799840346	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	5.0
12	10.0
13	12.0
14	32.0
15	78.0
16	188.0
17	472.0
18	1058.0
19	2174.0
20	3758.0
21	5994.0
22	9355.0
23	14002.0
24	20767.0
25	31293.0
26	42148.0
27	48996.0
28	51918.0
29	56478.0
30	65341.0
31	78564.0
32	96114.0
33	122962.0
34	192505.0
35	259055.0
36	209569.0
37	269819.0
38	421672.0
39	836493.0
40	258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.00339869317854	23.728165085819114	28.642437485304946	15.625998735697399
2	32.21215645251896	24.325259442496055	30.16326116859292	13.299322936392063
3	27.88719971053384	25.01527581648183	33.67219364948407	13.425330823500259
4	25.770759975389744	27.30059427853797	33.397721721084714	13.530924024987575
5	23.44834310187773	31.395076259410114	31.40025032628299	13.756330312429165
6	21.945505460576424	40.17571412682166	27.725008553049317	10.153771859552595
7	87.09658117371771	3.0547409235603773	8.102975897999782	1.7457020047221279
8	87.51652533603277	2.559755192721672	8.049897715385493	1.8738217558600705
9	82.38715958511727	5.656768594610805	9.373332507359846	2.5827393129120773
10	39.01228823283442	37.604695659274675	14.37327619098572	9.00973991690519
11	26.4970300152195	24.612613741476867	32.33584128919444	16.554514954109194
12	27.36433737450248	22.93653285426871	31.78432799782619	17.914801773402623
13	23.75037485586528	26.311397167004802	31.89896701690758	18.039260960222336
14	16.783018642127743	31.149959241024227	33.19987525923131	18.867146857616717
15	16.47292660709333	29.216230942187018	37.79420025821057	16.516642192509075
16	21.778773795427956	27.386441551347158	34.80337841928385	16.031406233941034
17	21.290018063477	26.668724560837877	30.886820982917833	21.154436392767288
18	21.67599641264697	27.412804653985155	33.68380890164768	17.227390031720198
19	22.78229638462957	29.05734837168244	30.319715095463295	17.840640148224697
20	24.34947548337048	29.215562185244266	30.150484391212956	16.2844779401723
21	23.116569262804585	27.244066718008426	32.94990095357701	16.68946306560998
22	21.712637253563067	23.975534759170067	33.43636883282907	20.8754591544378
23	18.41006908611196	28.152027459864026	33.263266377857526	20.174637076166487
24	18.429603828387116	28.00275387069479	35.108753957985165	18.458888342932926
25	19.70010122868249	29.38957978129536	31.840221999146806	19.070096990875342
26	19.32102163534303	31.94342175473374	29.02391052454479	19.71164608537844
27	18.17734167003392	30.47775292035598	32.92550892403344	18.419396485576673
28	17.099305478316083	29.148580897767477	35.03339560985705	18.718718014059384
29	18.84194528019508	27.76520436508216	33.554844404897835	19.83800594982493
30	20.07615381691265	28.097646961098054	32.386948398714296	19.439250823274996
31	21.742590525051636	27.718743356427737	30.545297371574026	19.993368746946597
32	22.68483385965678	28.364023410716726	30.640225659711124	18.31091706991537
33	21.78894594050457	28.15325938054804	30.08191920571386	19.975875473233533
34	19.152706072172247	28.517027959671843	31.719916144918926	20.61034982323698
35	19.791228161566046	28.603473593956124	31.459100937245253	20.146197307232573
36	22.493745362698565	28.40186097458301	29.62016013560983	19.48423352710859
37	19.420807210748542	30.397572482693274	31.120956308349047	19.06066399820914
38	19.801435504376485	30.73247892007721	29.082268367233443	20.38381720831286
39	18.896853744968485	29.986638940238468	30.18543574090526	20.931071573887785
40	21.116845213037806	28.342095222541193	30.24006262380803	20.300996940612976
41	18.65976180989563	28.343221550023724	30.735189145582055	22.261827494498597
42	20.886264858723337	29.033871483218427	29.31731883374421	20.76254482431403
43	20.189842497180663	28.845669200434198	29.474406319823505	21.490081982561634
44	20.285545135461998	29.52656936135824	29.827932358403036	20.35995314477673
45	19.386137442926874	30.052282713829754	29.244846699790077	21.31673314345329
46	20.59070948776034	29.51681958908758	29.26452223300055	20.627948690151534
47	19.825475556581765	29.109265029080365	30.257485502053438	20.80777391228443
48	19.95496801933904	29.04872492689431	30.451460213185634	20.544846840581016
49	20.25087536764033	28.002824266162445	30.808189245543616	20.93811112065361
50	19.063022246375688	29.136930447870046	30.5808118850076	21.21923542074667
51	18.14027845631187	30.095223949101264	30.12475484778388	21.63974274680299
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2005.0
1	3144.0
2	4283.0
3	24605.5
4	44928.0
5	30817.0
6	16706.0
7	16374.0
8	16042.0
9	17362.5
10	18683.0
11	19161.0
12	19639.0
13	19740.5
14	19842.0
15	19276.0
16	18710.0
17	17865.0
18	17020.0
19	16759.0
20	16498.0
21	18469.5
22	20441.0
23	22198.0
24	23955.0
25	26223.0
26	32440.0
27	36389.0
28	44797.0
29	53205.0
30	63181.5
31	73158.0
32	83036.0
33	92914.0
34	100549.5
35	108185.0
36	119029.0
37	129873.0
38	142011.0
39	154149.0
40	165630.0
41	177111.0
42	195623.0
43	214135.0
44	217083.0
45	220031.0
46	230983.0
47	241935.0
48	242451.0
49	242967.0
50	224764.0
51	206561.0
52	186805.0
53	167049.0
54	149611.5
55	132174.0
56	118577.0
57	104980.0
58	93176.5
59	81373.0
60	67215.0
61	53057.0
62	44718.5
63	36380.0
64	29225.5
65	22071.0
66	16645.0
67	11219.0
68	8799.5
69	6380.0
70	5094.0
71	3808.0
72	3126.0
73	2444.0
74	1798.0
75	856.0
76	560.0
77	446.0
78	332.0
79	239.5
80	147.0
81	106.0
82	65.0
83	47.5
84	30.0
85	17.0
86	4.0
87	4.0
88	4.0
89	4.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2841092.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.979463872148447
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.42688894897593	15.269682666167657
2	8.85289095604834	3.53752030040875
3	3.367567139062952	2.0184655797582765
4	1.7580601886130915	1.40500400093831
5	1.129781109785434	1.1286210433196928
6	0.8221266141244932	0.9855389391139221
7	0.5676756325804976	0.7939298354568747
8	0.46835516607009986	0.7485988095865316
9	0.35081461220952753	0.6308179083415819
>10	3.8092202532420987	17.936014794368717
>50	1.3531567940319464	19.396999485650714
>100	1.0877582083842496	33.967911961870186
>500	0.0026739266584272585	0.34394127244107625
>1k	0.0028521884356557427	0.7460074446050309
>5k	0.0	0.0
>10k+	1.7826177722848392E-4	1.090945957972725
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30631	1.0781417849193198	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08651602975194045	0.0
2	0.0	0.0	0.0	0.2428995611546546	0.0
3	0.0	0.0	0.0	0.3721104420412996	0.0
4	0.0	0.0	0.0	0.5288459507823048	0.0
5	0.0	0.0	0.0	0.8821256052250331	0.0
6	0.0	0.0	0.0	1.3043928179728077	0.0
7	0.0	0.0	0.0	1.6253609527604174	0.0
8	0.0	0.0	0.0	2.2959129799386995	0.0
9	0.0	0.0	0.0	2.577178070967079	0.0
10	0.0	0.0	0.0	2.972483819601759	0.0
11	0.0	0.0	0.0	3.4759522042932787	0.0
12	0.0	0.0	0.0	3.8524623630632164	0.0
13	0.0	0.0	0.0	4.022784197062256	0.0
14	0.0	0.0	0.0	4.107117967316793	0.0
15	0.0	0.0	0.0	4.207748288334204	0.0
16	0.0	0.0	0.0	4.410029664650071	0.0
17	0.0	0.0	0.0	4.67517419358472	0.0
18	0.0	0.0	0.0	4.98276718951727	0.0
19	0.0	0.0	0.0	5.186315684250985	0.0
20	0.0	0.0	0.0	5.394228697979509	0.0
21	0.0	0.0	0.0	5.639803286905176	0.0
22	0.0	0.0	0.0	5.9168094521402335	0.0
23	0.0	0.0	0.0	6.208387479180541	0.0
24	0.0	0.0	0.0	6.425592694639948	0.0
25	0.0	0.0	0.0	6.619848987642779	0.0
26	0.0	0.0	0.0	6.80076533952438	0.0
27	0.0	0.0	0.0	6.99938615152202	0.0
28	0.0	0.0	0.0	7.205609674026747	0.0
29	0.0	0.0	0.0	7.449107596656497	0.0
30	0.0	0.0	0.0	7.726958507503453	0.0
31	3.519773382910515E-5	0.0	0.0	7.965493549663298	0.0
32	3.519773382910515E-5	0.0	0.0	8.19040706883128	0.0
33	3.519773382910515E-5	0.0	0.0	8.417432452029008	0.0
34	3.519773382910515E-5	0.0	0.0	8.664450148041668	0.0
35	3.519773382910515E-5	0.0	0.0	8.92276631661347	0.0
36	3.519773382910515E-5	0.0	0.0	9.158978308340595	0.0
37	3.519773382910515E-5	0.0	0.0	9.421342216302746	0.0
38	3.519773382910515E-5	0.0	0.0	9.711723520392862	0.0
39	7.03954676582103E-5	0.0	0.0	10.141945420986016	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCACGA	20	7.0350413E-4	45.000004	38
GTCGTAA	25	3.892262E-5	45.0	20
CGTTTTT	14735	0.0	43.457752	1
ATTAGCG	255	0.0	43.235294	1
CGGTCTA	430	0.0	42.906975	31
CGACGGT	435	0.0	42.413795	28
GCGATAC	80	0.0	42.187504	9
TATCGCG	60	3.6379788E-12	41.250004	1
TCACGAC	460	0.0	41.08696	25
AGTACGG	390	0.0	40.961536	2
AGTTGCG	100	0.0	40.5	1
TAGTGCG	200	0.0	40.5	1
ACGGGAT	1365	0.0	40.054947	5
CCGCTCG	45	1.9299478E-8	40.0	19
TACGGGA	1370	0.0	39.744526	4
ACGACGG	465	0.0	39.67742	27
TATAGCG	125	0.0	39.600002	1
GCGATAT	205	0.0	39.512196	9
TGTTGCG	195	0.0	39.23077	1
CGTTAGG	385	0.0	39.15584	2
>>END_MODULE