FastQCFastQC Report
Thu 26 May 2016
SRR1547529_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547529_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences798888
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31160.39004215860045466No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC20130.251975245591372No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11530.1443256126015161No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7990.10001401948708706No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA253.8897328E-545.00000432
ACGCACT253.8897328E-545.00000424
CGACCCA253.8897328E-545.00000420
CGACAGG600.045.0000042
TTATCCG253.8897328E-545.0000041
CCCGTTG302.1646665E-645.00000420
TACGCCC302.1646665E-645.00000434
AGGTTCG302.1646665E-645.00000443
TATTACG302.1646665E-645.00000430
TTGAACG253.8897328E-545.00000434
TAAGGTC253.8897328E-545.00000443
CGCACTC253.8897328E-545.00000425
ACGTACG253.8897328E-545.00000438
TATACGA253.8897328E-545.00000431
TGTACGT253.8897328E-545.00000419
TAATGCG502.1827873E-1145.0000041
ATCGCGC253.8897328E-545.00000410
TGCGTAG600.045.0000041
CTTACCG253.8897328E-545.00000422
AAGTACG207.031994E-445.01