##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547529_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	798888
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.411439400767065	31.0	31.0	33.0	30.0	34.0
2	31.83100384534503	31.0	31.0	34.0	30.0	34.0
3	31.993259380538948	33.0	31.0	34.0	30.0	34.0
4	35.70060634281651	37.0	35.0	37.0	33.0	37.0
5	35.60848329177557	37.0	35.0	37.0	33.0	37.0
6	35.660599483281764	37.0	35.0	37.0	33.0	37.0
7	35.96300357496921	37.0	35.0	37.0	35.0	37.0
8	35.89917986000541	37.0	35.0	37.0	35.0	37.0
9	37.56481384123932	39.0	37.0	39.0	35.0	39.0
10	37.178080031243425	39.0	37.0	39.0	33.0	39.0
11	36.87955382982346	39.0	37.0	39.0	33.0	39.0
12	36.1857769800022	38.0	35.0	39.0	32.0	39.0
13	35.8695086169776	38.0	35.0	39.0	31.0	39.0
14	36.93187405493636	39.0	35.0	41.0	31.0	41.0
15	37.20123096103584	39.0	35.0	41.0	32.0	41.0
16	37.30931870299717	39.0	35.0	41.0	33.0	41.0
17	37.202675468901774	39.0	35.0	41.0	32.0	41.0
18	37.18228462562963	39.0	35.0	41.0	32.0	41.0
19	37.14735983016393	39.0	35.0	41.0	32.0	41.0
20	37.036981404151774	38.0	35.0	41.0	32.0	41.0
21	36.88201349876328	38.0	35.0	41.0	32.0	41.0
22	36.792718128198196	38.0	35.0	41.0	31.0	41.0
23	36.728283564154175	38.0	35.0	41.0	31.0	41.0
24	36.67326333603709	38.0	35.0	41.0	31.0	41.0
25	36.59006268713512	38.0	35.0	40.0	31.0	41.0
26	36.571615795956376	38.0	35.0	40.0	31.0	41.0
27	36.540585163377095	38.0	35.0	40.0	31.0	41.0
28	36.50297663752616	38.0	35.0	40.0	31.0	41.0
29	36.486429887543686	38.0	35.0	40.0	31.0	41.0
30	36.438171558466266	38.0	35.0	40.0	31.0	41.0
31	36.31109367020158	38.0	35.0	40.0	31.0	41.0
32	36.15086345019577	38.0	35.0	40.0	30.0	41.0
33	36.05718573817607	38.0	35.0	40.0	30.0	41.0
34	35.98922877800142	38.0	35.0	40.0	30.0	41.0
35	35.90207888965662	38.0	35.0	40.0	30.0	41.0
36	35.80007710717898	38.0	35.0	40.0	30.0	41.0
37	35.73291375011266	38.0	35.0	40.0	29.0	41.0
38	35.62618664944272	38.0	34.0	40.0	29.0	41.0
39	35.59478425010765	38.0	35.0	40.0	29.0	41.0
40	35.484198035269024	38.0	34.0	40.0	28.0	41.0
41	35.4816232062567	37.0	34.0	40.0	29.0	41.0
42	35.436559317451255	37.0	34.0	40.0	28.0	41.0
43	35.37014825607594	37.0	34.0	40.0	28.0	41.0
44	35.22412528414496	37.0	34.0	40.0	28.0	41.0
45	35.1567291034538	37.0	34.0	40.0	27.0	41.0
46	35.1175246092068	37.0	34.0	40.0	27.0	41.0
47	35.07027142728393	37.0	34.0	40.0	27.0	41.0
48	35.001553409238845	36.0	34.0	40.0	27.0	41.0
49	34.94683860566187	36.0	34.0	40.0	27.0	41.0
50	34.82403791269865	36.0	34.0	40.0	27.0	41.0
51	34.386359539760264	35.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	6.0
14	11.0
15	17.0
16	57.0
17	147.0
18	288.0
19	639.0
20	1028.0
21	1657.0
22	2580.0
23	3597.0
24	5266.0
25	7380.0
26	9603.0
27	11871.0
28	13344.0
29	15857.0
30	18835.0
31	23319.0
32	29112.0
33	37854.0
34	68890.0
35	109587.0
36	53639.0
37	69210.0
38	106897.0
39	208105.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.13718568810647	24.509568299936912	29.13599903866374	13.217246973292877
2	29.816945554320505	26.87798539970559	30.68477683980733	12.620292206166573
3	30.508782207268105	25.210793002273157	31.0830804818698	13.197344308588937
4	27.06186599373129	28.47220135989025	29.799296021469846	14.666636624908621
5	24.140680545958883	33.56077447652236	28.36104685512863	13.93749812239012
6	22.22777160252751	41.93353761728803	25.15796957771302	10.680721202471435
7	88.05064039014229	4.5798660137591245	5.5833859064099105	1.7861076896886672
8	88.15215649753156	4.537306856530578	5.24554130241035	2.0649953435275035
9	83.55439060293808	7.044041217292035	6.468491202771853	2.933076976998027
10	46.70529536055116	32.5557524959694	11.466062827329988	9.272889316149447
11	40.70082414556233	22.01560168634402	22.89044271537437	14.39313145271928
12	37.75047315768919	21.698661138983187	24.856049909374033	15.694815793953595
13	24.172349565896596	35.3450045563333	23.85490832256837	16.62773755520173
14	16.854177306455973	37.84460399955939	27.112686634421845	18.188532059562792
15	16.326819278797526	28.091547250678445	40.14930753747709	15.432325933046936
16	20.461191055567237	23.880944512872894	38.20673235797759	17.45113207358228
17	18.909158730635582	24.200013018095152	27.64405022981945	29.246778021449813
18	21.951011906550104	26.931810216200518	31.236668969868113	19.880508907381262
19	26.84218563803687	27.847708314557234	25.370139493896517	19.93996655350938
20	29.40987973282863	26.527498222529317	25.962713171308117	18.099908873333934
21	24.154700033045934	28.73894713651976	28.3781956920119	18.728157138422407
22	23.885701124563145	24.73100109151721	27.663076676580445	23.7202211073392
23	20.219104555331914	29.697404392105025	26.74142057459869	23.34207047796437
24	20.51000891238822	26.465411922572375	34.552778361923075	18.47180080311633
25	19.960620262164408	27.948723725979114	31.023873183725375	21.066782828131103
26	17.877224341835152	35.53276554410631	26.104660478064513	20.485349635994034
27	18.918671954016084	33.40405663872783	28.857862428776	18.819408978480087
28	16.996625309179763	29.794539409779592	34.99339081323039	18.215444467810258
29	18.303066262104327	26.750307928019947	34.01315328306346	20.93347252681227
30	18.957100369513626	31.485890387638815	29.49161834950581	20.065390893341746
31	27.195927339001212	28.214092588698293	25.123922251930182	19.466057820370313
32	27.41247834489941	29.204469212204963	25.778457055306873	17.60459538758875
33	25.680821341664917	29.91433091997877	24.116772313515785	20.288075424840528
34	20.044111314727473	30.25242587196203	27.774231181342063	21.929231631968435
35	21.74147064419543	27.075259610859092	29.4787254283454	21.704544316600074
36	26.413339541963328	28.38470473958803	26.578694385195423	18.62326133325322
37	20.97640720601636	32.14693423859164	28.27329988684271	18.603358668549284
38	20.56583651275273	32.273735492334346	25.359624878581226	21.8008031163317
39	21.46971790789197	30.152662200458636	26.787860125574547	21.589759766074845
40	22.95741080101341	27.597861026827292	26.615745886782626	22.82898228537667
41	18.148851904146763	27.007665655260816	28.18380048267091	26.65968195792151
42	22.38611670221608	27.949975465897598	24.464881184846938	25.199026647039386
43	23.359845184806883	26.85157368742552	26.308819258769688	23.479761868997905
44	20.02095412623547	31.445459188271695	26.931184346241277	21.602402339251558
45	19.57258088743353	34.62149888344799	24.41105636835201	21.394863860766467
46	22.216380769269286	32.21840858793723	25.63951392435485	19.92569671843863
47	21.457826378666347	28.67323079079921	27.22859775087371	22.64034507966073
48	22.316895484723766	26.117928921200466	30.12049248455353	21.444683109522238
49	22.098592042939686	25.298414796567226	30.09107659646909	22.51191656402399
50	20.0367260492084	30.319268783609214	28.809420093930566	20.834585073251823
51	19.844208449745146	30.729964650864698	26.004521284585575	23.421305614804577
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1054.0
1	1495.0
2	1936.0
3	3711.0
4	5486.0
5	4000.0
6	2514.0
7	2520.5
8	2527.0
9	2726.0
10	2925.0
11	2994.0
12	3063.0
13	3080.5
14	3098.0
15	2853.0
16	2608.0
17	2544.0
18	2480.0
19	2513.5
20	2547.0
21	2785.0
22	3023.0
23	3118.5
24	3214.0
25	4817.0
26	7737.0
27	9054.0
28	10565.0
29	12076.0
30	14463.5
31	16851.0
32	18888.0
33	20925.0
34	22314.5
35	23704.0
36	25448.5
37	27193.0
38	30757.0
39	34321.0
40	43481.5
41	52642.0
42	61532.0
43	70422.0
44	73029.0
45	75636.0
46	74203.0
47	72770.0
48	70038.5
49	67307.0
50	62524.0
51	57741.0
52	51382.5
53	45024.0
54	41579.0
55	38134.0
56	35553.5
57	32973.0
58	30409.0
59	27845.0
60	26516.0
61	25187.0
62	20888.0
63	16589.0
64	14006.0
65	11423.0
66	9199.5
67	6976.0
68	5411.5
69	3847.0
70	3334.0
71	2821.0
72	2328.5
73	1836.0
74	1498.0
75	939.5
76	719.0
77	571.0
78	423.0
79	316.0
80	209.0
81	155.5
82	102.0
83	63.5
84	25.0
85	35.5
86	46.0
87	25.0
88	4.0
89	4.0
90	4.0
91	4.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	798888.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.704615324727957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.96029203547221	14.769218884516883
2	7.591310218024712	2.839850748776579
3	2.4419477283947275	1.3702707870814996
4	1.2261858096701317	0.9174133554607962
5	0.7417832921606451	0.693738556708758
6	0.4873766253416382	0.5469715377167642
7	0.3882058701290475	0.5082869027295595
8	0.32299106066027605	0.483313883438107
9	0.2715414199679669	0.4571170024708101
>10	4.272794484727409	20.842898571712066
>50	2.2879910022682006	31.423348386564697
>100	1.002177877026567	23.929249840221694
>500	0.0033766100977984065	0.42480200598960904
>1k	0.0020259660586790444	0.7935195366121689
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3116	0.39004215860045466	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2013	0.251975245591372	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1153	0.1443256126015161	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	799	0.10001401948708706	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2517399184866965E-4	0.0	0.0	0.08486796647339802	0.0
2	1.2517399184866965E-4	0.0	0.0	0.23032014500155215	0.0
3	1.2517399184866965E-4	0.0	0.0	0.3320866003745206	0.0
4	1.2517399184866965E-4	0.0	0.0	0.5170937603268543	0.0
5	1.2517399184866965E-4	0.0	0.0	0.8195141246332402	0.0
6	1.2517399184866965E-4	0.0	0.0	1.1485965492033927	0.0
7	1.2517399184866965E-4	0.0	0.0	1.3363575369763971	0.0
8	1.2517399184866965E-4	0.0	0.0	1.7969978269795015	0.0
9	1.2517399184866965E-4	0.0	0.0	1.9314346942249727	0.0
10	1.2517399184866965E-4	0.0	0.0	2.19893151480558	0.0
11	1.2517399184866965E-4	0.0	0.0	2.61663712560459	0.0
12	1.2517399184866965E-4	0.0	0.0	2.9682508687075035	0.0
13	1.2517399184866965E-4	0.0	0.0	3.1133275252601114	0.0
14	1.2517399184866965E-4	0.0	0.0	3.1671523417550396	0.0
15	2.503479836973393E-4	0.0	0.0	3.2546489620572596	0.0
16	2.503479836973393E-4	0.0	0.0	3.453800783088493	0.0
17	2.503479836973393E-4	0.0	0.0	3.6946355434053335	0.0
18	2.503479836973393E-4	0.0	0.0	3.9830364206246682	0.0
19	2.503479836973393E-4	0.0	0.0	4.1398794324110515	0.0
20	2.503479836973393E-4	0.0	0.0	4.300853185928441	0.0
21	2.503479836973393E-4	0.0	0.0	4.493245611399845	0.0
22	2.503479836973393E-4	0.0	0.0	4.687014950781586	0.0
23	2.503479836973393E-4	0.0	0.0	4.896681387128108	0.0
24	2.503479836973393E-4	0.0	0.0	5.06867045192818	0.0
25	2.503479836973393E-4	0.0	0.0	5.216500936301459	0.0
26	2.503479836973393E-4	0.0	0.0	5.3556944152371795	0.0
27	2.503479836973393E-4	0.0	0.0	5.488128498613072	0.0
28	2.503479836973393E-4	0.0	0.0	5.633705851133075	0.0
29	2.503479836973393E-4	0.0	0.0	5.8071970038353316	0.0
30	2.503479836973393E-4	0.0	0.0	5.993080381730605	0.0
31	2.503479836973393E-4	0.0	0.0	6.183470023332432	0.0
32	2.503479836973393E-4	0.0	0.0	6.35395700023032	0.0
33	2.503479836973393E-4	0.0	0.0	6.521314627331991	0.0
34	2.503479836973393E-4	0.0	0.0	6.697058911887524	0.0
35	2.503479836973393E-4	0.0	0.0	6.9044722163807695	0.0
36	2.503479836973393E-4	0.0	0.0	7.093109422096714	0.0
37	2.503479836973393E-4	0.0	0.0	7.296141636875257	0.0
38	2.503479836973393E-4	0.0	0.0	7.486656452468932	0.0
39	2.503479836973393E-4	0.0	0.0	7.687310361402349	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGTA	25	3.8897328E-5	45.000004	32
ACGCACT	25	3.8897328E-5	45.000004	24
CGACCCA	25	3.8897328E-5	45.000004	20
CGACAGG	60	0.0	45.000004	2
TTATCCG	25	3.8897328E-5	45.000004	1
CCCGTTG	30	2.1646665E-6	45.000004	20
TACGCCC	30	2.1646665E-6	45.000004	34
AGGTTCG	30	2.1646665E-6	45.000004	43
TATTACG	30	2.1646665E-6	45.000004	30
TTGAACG	25	3.8897328E-5	45.000004	34
TAAGGTC	25	3.8897328E-5	45.000004	43
CGCACTC	25	3.8897328E-5	45.000004	25
ACGTACG	25	3.8897328E-5	45.000004	38
TATACGA	25	3.8897328E-5	45.000004	31
TGTACGT	25	3.8897328E-5	45.000004	19
TAATGCG	50	2.1827873E-11	45.000004	1
ATCGCGC	25	3.8897328E-5	45.000004	10
TGCGTAG	60	0.0	45.000004	1
CTTACCG	25	3.8897328E-5	45.000004	22
AAGTACG	20	7.031994E-4	45.0	1
>>END_MODULE