FastQCFastQC Report
Thu 26 May 2016
SRR1547528_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547528_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4574463
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321590.7030114791615977No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGATA253.892636E-545.027
GCCCGTA302.1669275E-644.99999619
CGTAAGG6250.042.1200032
CGTTTTT167750.041.2311
CACGACG9850.040.659926
TCGTTAG1550.040.645161
TCACGAC9900.040.45454425
CGACGGT9900.040.22727228
CGGTCTA10050.039.85074631
TCGATTG1400.038.5714261
GCGTAAG3700.038.310811
CGAGGGA32850.038.01374
GCGGGAT33250.037.9624065
CGTTAGG6300.037.857142
AGGGATT101000.037.7599036
ATAGGGA66800.037.7245524
TAGGGAC45700.037.6641125
ACGGTCT10700.037.64018630
TCGATAG2400.037.4999961
AACGCGT301.1404539E-437.49999613
Produced by FastQC (version 0.11.5)