##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547528_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4574463
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.406528154233623	31.0	31.0	33.0	30.0	34.0
2	31.83254668362166	31.0	31.0	34.0	30.0	34.0
3	31.959598755088848	33.0	31.0	34.0	30.0	34.0
4	35.700363080868726	37.0	35.0	37.0	33.0	37.0
5	35.61243363428669	37.0	35.0	37.0	33.0	37.0
6	35.6533877309752	37.0	35.0	37.0	33.0	37.0
7	35.979752377492176	37.0	35.0	37.0	35.0	37.0
8	35.92782125464781	37.0	35.0	37.0	35.0	37.0
9	37.57414629870217	39.0	37.0	39.0	35.0	39.0
10	37.14792512257723	39.0	37.0	39.0	33.0	39.0
11	36.92168632689783	39.0	37.0	39.0	33.0	39.0
12	36.53875263610177	39.0	35.0	39.0	32.0	39.0
13	36.40236460541926	39.0	35.0	39.0	32.0	39.0
14	37.44663471974743	40.0	36.0	41.0	32.0	41.0
15	37.649790806046525	40.0	36.0	41.0	32.0	41.0
16	37.71244843383803	40.0	36.0	41.0	33.0	41.0
17	37.638149002407495	40.0	36.0	41.0	32.0	41.0
18	37.53037176166907	39.0	36.0	41.0	32.0	41.0
19	37.50035512365058	39.0	36.0	41.0	32.0	41.0
20	37.39026373150247	39.0	35.0	41.0	32.0	41.0
21	37.270203081760634	39.0	35.0	41.0	32.0	41.0
22	37.23065964245421	39.0	35.0	41.0	32.0	41.0
23	37.160478071415156	39.0	35.0	41.0	32.0	41.0
24	37.09467581222102	39.0	35.0	41.0	32.0	41.0
25	36.99220586110326	39.0	35.0	41.0	31.0	41.0
26	36.99811650023183	39.0	35.0	41.0	31.0	41.0
27	36.96079364944038	39.0	35.0	41.0	31.0	41.0
28	36.902513584654635	39.0	35.0	41.0	31.0	41.0
29	36.853494279000614	39.0	35.0	41.0	31.0	41.0
30	36.757693744599095	39.0	35.0	41.0	31.0	41.0
31	36.608111596924054	39.0	35.0	41.0	30.0	41.0
32	36.46990062002906	39.0	35.0	41.0	30.0	41.0
33	36.331768559500865	39.0	35.0	41.0	30.0	41.0
34	36.20656588543836	39.0	35.0	41.0	30.0	41.0
35	36.0912719154139	39.0	35.0	41.0	29.0	41.0
36	36.016427283377304	39.0	35.0	41.0	29.0	41.0
37	35.95757491097862	39.0	35.0	41.0	29.0	41.0
38	35.828526539617876	38.0	35.0	41.0	28.0	41.0
39	35.75331508856887	38.0	35.0	41.0	28.0	41.0
40	35.65879995094506	38.0	35.0	41.0	27.0	41.0
41	35.594446823594375	38.0	35.0	40.0	27.0	41.0
42	35.57379784250086	38.0	35.0	40.0	27.0	41.0
43	35.539874953628434	38.0	35.0	40.0	27.0	41.0
44	35.439602856116664	38.0	34.0	40.0	27.0	41.0
45	35.37580126891397	38.0	34.0	40.0	27.0	41.0
46	35.36099188035842	38.0	34.0	40.0	27.0	41.0
47	35.30835663114993	38.0	34.0	40.0	27.0	41.0
48	35.20761562614016	38.0	34.0	40.0	26.0	41.0
49	35.127073057537025	38.0	34.0	40.0	26.0	41.0
50	35.024118240764	37.0	34.0	40.0	26.0	41.0
51	34.5951432987872	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	9.0
11	9.0
12	17.0
13	19.0
14	47.0
15	147.0
16	348.0
17	886.0
18	1861.0
19	3667.0
20	6173.0
21	9638.0
22	14802.0
23	21503.0
24	31739.0
25	46518.0
26	60872.0
27	71313.0
28	78375.0
29	88078.0
30	102965.0
31	124848.0
32	153830.0
33	198630.0
34	318186.0
35	424145.0
36	329453.0
37	437921.0
38	688687.0
39	1359373.0
40	399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.02140600984203	23.6478904737015	29.14696216801841	14.183741348438057
2	31.713361765085867	24.776175913981596	29.869210877866976	13.641251443065558
3	29.644091557850615	24.14742451736958	32.54620269089508	13.662281233884721
4	26.82181493215706	26.98594348669997	31.339919024375103	14.852322556767867
5	24.486896057526312	31.390416754928392	29.52630724087177	14.59637994667352
6	22.78676207458668	40.8086369919267	26.094407146806088	10.310193786680536
7	87.0290786044176	3.668430589557725	7.295522993627886	2.0069678123967774
8	87.28217935088774	3.0705462040025244	7.438053384626786	2.209221060482946
9	81.71026850583335	6.156897541853547	8.955608559955563	3.177225392357529
10	39.375572608194666	36.39485115520663	14.471797017485988	9.757779219112713
11	30.886991544144088	23.030178624245075	29.105492819594343	16.97733701201649
12	29.304095365947873	21.64767755253458	31.162543013245493	17.885684068272056
13	24.889129062799284	24.778908475158723	31.074620124810277	19.257342337231716
14	18.13373504168686	29.725631183376063	32.83607278056462	19.304560994372455
15	17.697071765582102	27.626368384660672	37.49769098580533	17.178868863951898
16	23.840678129870106	25.56575493123455	33.80707199948934	16.786494939406
17	23.403512062508756	24.661014855732795	29.72847304700027	22.207000034758178
18	23.035709328067576	25.771483997138024	32.23289815657051	18.959908518223887
19	24.762228921733545	27.360894601180508	28.903436315912927	18.973440161173016
20	25.526537213220436	27.57036181077429	28.790439446116405	18.11266152988886
21	24.785532203452078	25.349970040199253	32.386839723045085	17.47765803330358
22	23.228759310109186	22.271488478538355	32.64490280061288	21.854849410739575
23	20.341185402527028	26.185324922291425	32.22765163911043	21.24583803607112
24	20.519129786381484	26.494366661179686	33.41023853510237	19.57626501733646
25	21.082583901104897	27.839333272561174	30.481654349373905	20.59642847696003
26	20.61651826673426	30.281674592187102	27.943017573866047	21.158789567212587
27	19.24538464952061	29.42703875842913	31.987098813565655	19.340477778484598
28	18.509320110360495	27.898378454476514	33.327890071468495	20.264411363694492
29	19.91302585680549	26.11386298238722	33.20588668003217	20.76722448077512
30	21.09782066222855	26.722699473140345	32.30875405484753	19.870725809783572
31	23.33911106068625	26.04428104457288	30.16633865002297	20.450269244717905
32	24.230647400580132	26.644701246900453	29.361763337029945	19.76288801548947
33	23.67978492776092	26.542853226706608	29.163095209208162	20.61426663632431
34	20.106884676955524	27.730424314285635	31.182982570850392	20.97970843790845
35	21.087917860522644	27.624291638166053	30.364831893929406	20.922958607381894
36	23.829726024672183	27.226867940564826	29.19503338424641	19.748372650516576
37	21.317999511636668	29.609027332825733	29.286956742244936	19.786016413292664
38	22.652582390545074	29.32112468720372	27.4241151365745	20.6021777856767
39	21.40349588574659	27.836994200193555	28.606089938862766	22.15341997519709
40	22.667207057965054	26.87030149768399	29.27182928356837	21.19066216078259
41	20.649221558902106	26.303983658846953	29.508075592697985	23.53871918955296
42	21.31093857355497	27.761247604363614	28.694996549321743	22.232817272759668
43	21.302150656809335	26.871175917260672	29.857887144348965	21.96878628158103
44	21.461469903680495	27.828446748831503	29.404456872861363	21.305626474626642
45	20.243403433364744	29.013853647958243	28.209584381817056	22.533158536859954
46	21.541085806137243	28.11094985356751	28.90350189738118	21.444462442914062
47	20.671213211255616	27.722707561521432	30.2612350345822	21.344844192640757
48	21.255872000713527	27.55376969930678	29.892623462032592	21.2977348379471
49	21.71179436799467	26.55242812107126	30.29288902325803	21.44288848767604
50	20.4117073413863	28.310689145370727	29.351729372387535	21.925874140855438
51	19.590321312031598	28.676939785063293	29.248504141360414	22.484234761544688
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3720.0
1	6032.5
2	8345.0
3	29962.0
4	51579.0
5	36033.0
6	20487.0
7	20089.0
8	19691.0
9	20580.5
10	21470.0
11	21641.5
12	21813.0
13	21592.5
14	21372.0
15	20185.0
16	18998.0
17	19584.0
18	20170.0
19	20394.5
20	20619.0
21	22930.5
22	25242.0
23	30068.5
24	34895.0
25	37825.0
26	50154.5
27	59554.0
28	70026.5
29	80499.0
30	92733.0
31	104967.0
32	123480.0
33	141993.0
34	151962.0
35	161931.0
36	177638.5
37	193346.0
38	206128.0
39	218910.0
40	240198.5
41	261487.0
42	285496.0
43	309505.0
44	320764.0
45	332023.0
46	342464.0
47	352905.0
48	355790.0
49	358675.0
50	345937.0
51	333199.0
52	305493.5
53	277788.0
54	255771.5
55	233755.0
56	223591.0
57	213427.0
58	196819.5
59	180212.0
60	158651.0
61	137090.0
62	118976.5
63	100863.0
64	84120.0
65	67377.0
66	56512.5
67	45648.0
68	38565.5
69	31483.0
70	25525.0
71	19567.0
72	16107.0
73	12647.0
74	10332.0
75	6097.0
76	4177.0
77	3039.5
78	1902.0
79	1636.0
80	1370.0
81	1026.0
82	682.0
83	435.5
84	189.0
85	122.0
86	55.0
87	46.0
88	37.0
89	25.0
90	13.0
91	10.5
92	8.0
93	5.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4574463.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.723879453229317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.60226453576365	12.202086528579601
2	8.972332913972677	2.8215976230709616
3	3.385809853291566	1.5971419795413788
4	1.6553606006511516	1.0411476214505593
5	0.9353993391640981	0.7354053224823323
6	0.643178160616014	0.6067953518685579
7	0.49498592082937676	0.5448169265116781
8	0.3466487195010341	0.4360530142440451
9	0.2721779821691166	0.3851724403305349
>10	2.663331450966888	9.553520371612644
>50	1.115393745398237	12.906334409056516
>100	1.9012645106884611	54.68255095920681
>500	0.008324806574790022	0.9060599725640356
>1k	0.0033863619965247547	0.8678332448594435
>5k	0.0	0.0
>10k+	1.4109841652186478E-4	0.7134842346209951
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32159	0.7030114791615977	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.558146825102749E-5	0.0	0.0	0.05615959731229655	0.0
2	6.558146825102749E-5	0.0	0.0	0.1493290032075896	0.0
3	6.558146825102749E-5	0.0	0.0	0.22044117528112042	0.0
4	6.558146825102749E-5	0.0	0.0	0.32423477903307996	0.0
5	6.558146825102749E-5	0.0	0.0	0.553310847633919	0.0
6	8.744195766803666E-5	0.0	0.0	0.8160302094475351	0.0
7	8.744195766803666E-5	0.0	0.0	0.9978438999288004	0.0
8	8.744195766803666E-5	0.0	0.0	1.388337822384835	0.0
9	8.744195766803666E-5	0.0	0.0	1.5563138230651334	0.0
10	8.744195766803666E-5	0.0	0.0	1.833832736214065	0.0
11	8.744195766803666E-5	0.0	0.0	2.1836005668862115	0.0
12	8.744195766803666E-5	0.0	0.0	2.4689892562252664	0.0
13	8.744195766803666E-5	0.0	0.0	2.5888065987198936	0.0
14	8.744195766803666E-5	0.0	0.0	2.649709922235681	0.0
15	8.744195766803666E-5	0.0	0.0	2.7268993103671404	0.0
16	8.744195766803666E-5	0.0	0.0	2.871069238072316	0.0
17	8.744195766803666E-5	0.0	0.0	3.0518336250615645	0.0
18	8.744195766803666E-5	0.0	0.0	3.2668971199461008	0.0
19	8.744195766803666E-5	0.0	0.0	3.415067517214589	0.0
20	8.744195766803666E-5	0.0	0.0	3.572856529826561	0.0
21	8.744195766803666E-5	0.0	0.0	3.75851766644522	0.0
22	8.744195766803666E-5	0.0	0.0	3.9697555756817793	0.0
23	8.744195766803666E-5	0.0	0.0	4.183507441201295	0.0
24	8.744195766803666E-5	0.0	0.0	4.34551552827075	0.0
25	3.93488809506165E-4	0.0	0.0	4.48870173395216	0.0
26	3.93488809506165E-4	0.0	0.0	4.630357705374379	0.0
27	3.93488809506165E-4	0.0	0.0	4.788015555049849	0.0
28	3.93488809506165E-4	0.0	0.0	4.952909226722349	0.0
29	3.93488809506165E-4	0.0	0.0	5.153129449292737	0.0
30	4.1534929892317416E-4	0.0	0.0	5.376237604282732	0.0
31	4.1534929892317416E-4	0.0	0.0	5.575015034551597	0.0
32	4.1534929892317416E-4	0.0	0.0	5.759430123273486	0.0
33	4.1534929892317416E-4	0.0	0.0	5.946337307788914	0.0
34	4.1534929892317416E-4	0.0	0.0	6.148634276853917	0.0
35	4.1534929892317416E-4	0.0	0.0	6.3679387066853534	0.0
36	4.1534929892317416E-4	0.0	0.0	6.5749138204855955	0.0
37	4.1534929892317416E-4	0.0	0.0	6.79883081358402	0.0
38	4.1534929892317416E-4	0.0	0.0	7.050641791178549	0.0
39	4.1534929892317416E-4	0.0	0.0	7.480572036542869	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGATA	25	3.892636E-5	45.0	27
GCCCGTA	30	2.1669275E-6	44.999996	19
CGTAAGG	625	0.0	42.120003	2
CGTTTTT	16775	0.0	41.231	1
CACGACG	985	0.0	40.6599	26
TCGTTAG	155	0.0	40.64516	1
TCACGAC	990	0.0	40.454544	25
CGACGGT	990	0.0	40.227272	28
CGGTCTA	1005	0.0	39.850746	31
TCGATTG	140	0.0	38.571426	1
GCGTAAG	370	0.0	38.31081	1
CGAGGGA	3285	0.0	38.0137	4
GCGGGAT	3325	0.0	37.962406	5
CGTTAGG	630	0.0	37.85714	2
AGGGATT	10100	0.0	37.759903	6
ATAGGGA	6680	0.0	37.724552	4
TAGGGAC	4570	0.0	37.664112	5
ACGGTCT	1070	0.0	37.640186	30
TCGATAG	240	0.0	37.499996	1
AACGCGT	30	1.1404539E-4	37.499996	13
>>END_MODULE