FastQCFastQC Report
Thu 26 May 2016
SRR1547527_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547527_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2576239
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107670.41793482669892046No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC45700.17739037410737124No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC41090.15949607159894716No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG33360.12949109147093885No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAATC900.045.0000048
CTATACG453.8562575E-1045.0000041
CCGGATA302.1665546E-645.00000420
CCGAATC302.1665546E-645.00000432
TATCGCG302.1665546E-645.0000041
AATACCG302.1665546E-645.0000041
AATCCCG406.8212103E-945.035
ACCGGAT351.2127202E-745.010
GTCGAAC207.03492E-445.037
CATGCGT207.03492E-445.014
TAGACGT207.03492E-445.010
ACCGCGA207.03492E-445.024
CCAATCG351.2127202E-745.024
CATTACG406.8212103E-945.028
CGCTCAC207.03492E-445.022
AGCGCGA406.8212103E-945.034
GACGATA207.03492E-445.013
CGTCCCA207.03492E-445.043
TATCGTG207.03492E-445.01
CGATACA351.2127202E-745.010