##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547527_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2576239
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.383723326911827	31.0	31.0	33.0	30.0	34.0
2	31.80280517451991	31.0	31.0	34.0	30.0	34.0
3	31.961335109048502	33.0	31.0	34.0	30.0	34.0
4	35.67325314149813	37.0	35.0	37.0	33.0	37.0
5	35.56356145528424	37.0	35.0	37.0	33.0	37.0
6	35.60361790967375	37.0	35.0	37.0	33.0	37.0
7	35.96454676759416	37.0	35.0	37.0	35.0	37.0
8	35.90854187053297	37.0	35.0	37.0	35.0	37.0
9	37.541094983811675	39.0	37.0	39.0	35.0	39.0
10	37.12336471887895	39.0	37.0	39.0	33.0	39.0
11	36.87069949643647	39.0	37.0	39.0	32.0	39.0
12	36.24479910443092	38.0	35.0	39.0	32.0	39.0
13	35.97041307114751	38.0	35.0	39.0	31.0	39.0
14	37.00726485392077	39.0	35.0	41.0	31.0	41.0
15	37.274125964244774	39.0	35.0	41.0	32.0	41.0
16	37.37310591136925	39.0	35.0	41.0	32.0	41.0
17	37.27658186992744	39.0	35.0	41.0	32.0	41.0
18	37.22577253119761	39.0	35.0	41.0	32.0	41.0
19	37.18993501767499	39.0	35.0	41.0	32.0	41.0
20	37.09486348122204	39.0	35.0	41.0	32.0	41.0
21	36.96485729778953	39.0	35.0	41.0	32.0	41.0
22	36.87387311503319	38.0	35.0	41.0	31.0	41.0
23	36.8376548138585	38.0	35.0	41.0	31.0	41.0
24	36.76760812952525	38.0	35.0	41.0	31.0	41.0
25	36.69255492211709	38.0	35.0	41.0	31.0	41.0
26	36.69993311955917	38.0	35.0	41.0	31.0	41.0
27	36.651600259137446	38.0	35.0	41.0	31.0	41.0
28	36.60188010506789	38.0	35.0	41.0	31.0	41.0
29	36.588112360693245	38.0	35.0	41.0	31.0	41.0
30	36.49967763084093	38.0	35.0	40.0	31.0	41.0
31	36.343034167249236	38.0	35.0	40.0	30.0	41.0
32	36.19777551694544	38.0	35.0	40.0	30.0	41.0
33	36.133518279942194	38.0	35.0	41.0	30.0	41.0
34	36.03865208158094	38.0	35.0	41.0	30.0	41.0
35	35.96985644577231	38.0	35.0	41.0	30.0	41.0
36	35.906524200588535	38.0	35.0	40.0	30.0	41.0
37	35.82737393541515	38.0	35.0	40.0	29.0	41.0
38	35.73380420061959	38.0	35.0	40.0	29.0	41.0
39	35.72052825844186	38.0	35.0	40.0	29.0	41.0
40	35.648105241788514	38.0	35.0	40.0	29.0	41.0
41	35.6152173769592	38.0	35.0	40.0	29.0	41.0
42	35.592061916615656	38.0	35.0	40.0	29.0	41.0
43	35.5159105968041	38.0	34.0	40.0	28.0	41.0
44	35.36235341519168	37.0	34.0	40.0	28.0	41.0
45	35.30222079550849	37.0	34.0	40.0	27.0	41.0
46	35.264172695157555	37.0	34.0	40.0	27.0	41.0
47	35.21998618916956	37.0	34.0	40.0	27.0	41.0
48	35.12774629993568	37.0	34.0	40.0	27.0	41.0
49	35.073203611931966	37.0	34.0	40.0	27.0	41.0
50	34.97762901656252	37.0	34.0	40.0	27.0	41.0
51	34.54907211636809	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	5.0
12	9.0
13	14.0
14	19.0
15	78.0
16	165.0
17	422.0
18	909.0
19	1754.0
20	3041.0
21	5057.0
22	7665.0
23	11314.0
24	16226.0
25	22840.0
26	31055.0
27	37607.0
28	43464.0
29	50770.0
30	61492.0
31	75850.0
32	93873.0
33	122528.0
34	214200.0
35	321502.0
36	178068.0
37	228886.0
38	354686.0
39	692450.0
40	289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.844638637952453	24.81163432429988	29.413342473272085	13.93038456447558
2	29.635720909434255	26.692360452582232	30.245175234130063	13.426743403853447
3	29.4337210173435	25.69812039954368	31.68118330636249	13.186975276750331
4	28.166020311003752	27.51456677738362	29.878904868686483	14.440508042926142
5	24.667315415999834	32.56988190924833	28.284681661911026	14.47812101284081
6	23.20266093324416	40.94806421298645	24.71637918686892	11.13289566690047
7	87.46525458235823	4.29676749711498	6.133708867849606	2.1042690526771777
8	87.43854898555608	4.1541565048894915	5.736463115417475	2.670831394136957
9	81.68741331840718	7.238730568087822	7.684147317077336	3.389708796427661
10	44.036946882645594	33.52918731530731	12.621266893327832	9.812598908719261
11	37.942093105492155	22.24463646424109	24.13887065602221	15.674399774244549
12	35.169563072370224	21.365680746235114	26.231960621665923	17.232795559728736
13	23.99043722263346	31.974362627069926	26.65117638542076	17.384023764875852
14	16.62803800423796	35.89814454326636	29.341687630689545	18.132129821806128
15	16.42087554764911	27.500010674475465	40.23271909166812	15.846394686207296
16	20.33883502268229	24.104518253158965	37.9921660994962	17.56448062466254
17	19.95269072473478	24.040665481735196	27.423736695236737	28.582907098293287
18	22.151322140531214	27.836625406260833	29.661960710943358	20.350091742264595
19	25.960052619341607	28.11233740347848	27.045588549820103	18.882021427359806
20	28.011570355079634	26.66091927030062	26.251524023974483	19.075986350645262
21	24.55971670330276	27.419855067794565	29.132545544105188	18.88788268479749
22	21.990040520308867	24.270030847293285	29.157193878362992	24.582734754034856
23	20.314846565089653	27.842447847424097	28.23138691712997	23.611318670356283
24	20.575575480380508	25.64366116652997	34.165463685628545	19.615299667460977
25	20.223744769021817	27.00525844069591	30.569252309277207	22.20174448100506
26	18.41991368036894	33.222771645022064	26.793360398627613	21.56395427598138
27	20.299941115711704	31.102937266301765	28.73852154245006	19.85860007553647
28	16.817927218709134	29.1791638896857	34.856432186610014	19.14647670499515
29	19.313697215204023	26.338084315934974	33.73786360659861	20.61035486226239
30	20.74559076234775	28.377374925230153	30.75762768904593	20.11940662337617
31	26.787421508641085	27.002269587565443	25.887427369898525	20.322881533894954
32	26.95840719746887	28.514862169231968	26.51046739064194	18.01626324265722
33	25.04115495495565	29.546948089831726	25.664583138443287	19.747313816769328
34	20.50333839368164	28.919327748706547	29.59247957972843	20.98485427788338
35	20.953645993248298	27.835499734302598	28.89712483973731	22.313729432711792
36	25.80078944538919	27.127646153947673	28.230765856739225	18.840798543923913
37	20.902214429639486	30.678908284518634	29.678690525219125	18.740186760622755
38	20.411887251144012	31.251487148513785	25.531870296195347	22.80475530414686
39	21.000613685298607	29.12241449648111	28.01731516369405	21.85965665452623
40	22.08374300676296	27.28508496300227	28.355366097633024	22.27580593260175
41	17.852807911067256	26.930498296159634	29.098037876144257	26.118655916628853
42	22.53129465084567	27.9631276446013	25.60593174779203	23.899645956761
43	23.178245496632883	26.105186669404507	27.257564224437253	23.459003609525357
44	20.78254385559725	29.421299809528538	28.241052169460986	21.555104165413226
45	20.49728305487185	31.320696565807754	25.300641749465015	22.88137862985538
46	22.013019754766542	29.832597053301345	27.479709762952893	20.674673428979222
47	21.32930989710194	28.502673859063542	28.021041526038537	22.14697471779598
48	21.557743672073904	26.501733728897047	29.601213241473328	22.339309357555724
49	21.563372031865054	25.237021875687777	30.760888256097356	22.438717836349813
50	19.902307200535354	27.577216244300317	30.125504660087827	22.394971895076505
51	19.115850664476394	28.77318447550868	27.943486609743896	24.167478250271035
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3206.0
1	4397.5
2	5589.0
3	11971.0
4	18353.0
5	13215.0
6	8077.0
7	8055.5
8	8034.0
9	8700.0
10	9366.0
11	9599.5
12	9833.0
13	9564.0
14	9295.0
15	8896.0
16	8497.0
17	8657.5
18	8818.0
19	8623.0
20	8428.0
21	9681.0
22	10934.0
23	12111.5
24	13289.0
25	16574.5
26	23397.0
27	26934.0
28	33212.5
29	39491.0
30	46024.0
31	52557.0
32	60424.0
33	68291.0
34	68764.5
35	69238.0
36	77624.0
37	86010.0
38	98518.5
39	111027.0
40	135557.5
41	160088.0
42	194005.0
43	227922.0
44	241796.5
45	255671.0
46	252475.5
47	249280.0
48	229147.5
49	209015.0
50	188922.5
51	168830.0
52	156715.0
53	144600.0
54	133213.0
55	121826.0
56	114695.5
57	107565.0
58	103161.0
59	98757.0
60	91630.5
61	84504.0
62	71151.5
63	57799.0
64	47423.0
65	37047.0
66	30246.5
67	23446.0
68	18249.0
69	13052.0
70	10753.5
71	8455.0
72	6800.5
73	5146.0
74	4393.5
75	2833.5
76	2026.0
77	1654.5
78	1283.0
79	980.5
80	678.0
81	539.0
82	400.0
83	229.0
84	58.0
85	35.5
86	13.0
87	7.5
88	2.0
89	3.5
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2576239.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.362061852791545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.75082741350693	11.483268342736393
2	10.598487818235844	3.256292508195935
3	4.16531206623488	1.9196334479303752
4	2.1106771972264533	1.2969741462027788
5	1.3158910218252013	1.0107399634405905
6	0.8641408719352632	0.796499131451684
7	0.5952405696818913	0.6400885714120863
8	0.4255601652843117	0.5229985264945431
9	0.3332423213662776	0.4607360237516934
>10	2.2170617660941696	7.173023828999625
>50	0.7535418907581433	8.568772464952987
>100	1.849298614625049	59.275834612279844
>500	0.01509109518901559	1.5165240481871634
>1k	0.005371406762191989	1.6555436642640178
>5k	0.0	0.0
>10k+	2.5578127439009475E-4	0.423070719700251
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10767	0.41793482669892046	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4570	0.17739037410737124	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	4109	0.15949607159894716	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3336	0.12949109147093885	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8816274421744256E-5	0.0	0.0	0.10414406427353984	0.0
2	3.8816274421744256E-5	0.0	0.0	0.3983714243903613	0.0
3	3.8816274421744256E-5	0.0	0.0	0.5909777780710563	0.0
4	3.8816274421744256E-5	0.0	0.0	0.9172673808602385	0.0
5	3.8816274421744256E-5	0.0	0.0	1.4973377858187846	0.0
6	3.8816274421744256E-5	0.0	0.0	2.0645988202181553	0.0
7	3.8816274421744256E-5	0.0	0.0	2.4011747357290996	0.0
8	3.8816274421744256E-5	0.0	0.0	3.229552848163544	0.0
9	3.8816274421744256E-5	0.0	0.0	3.4730473376111455	0.0
10	3.8816274421744256E-5	0.0	0.0	3.9294490922620144	0.0
11	3.8816274421744256E-5	0.0	0.0	4.599689702702273	0.0
12	3.8816274421744256E-5	0.0	0.0	5.158799319473077	0.0
13	3.8816274421744256E-5	0.0	0.0	5.37962510465838	0.0
14	3.8816274421744256E-5	0.0	0.0	5.469213066023766	0.0
15	3.8816274421744256E-5	0.0	0.0	5.613997769616872	0.0
16	3.8816274421744256E-5	0.0	0.0	5.927283920474769	0.0
17	3.8816274421744256E-5	0.0	0.0	6.2820258524150905	0.0
18	3.8816274421744256E-5	0.0	0.0	6.7524790984066305	0.0
19	3.8816274421744256E-5	0.0	0.0	7.0036592101897375	0.0
20	3.8816274421744256E-5	0.0	0.0	7.277702107607253	0.0
21	3.8816274421744256E-5	0.0	0.0	7.602128529224191	0.0
22	3.8816274421744256E-5	0.0	0.0	7.92791352044589	0.0
23	3.8816274421744256E-5	0.0	0.0	8.261888745570579	0.0
24	3.8816274421744256E-5	0.0	0.0	8.522928191056808	0.0
25	3.8816274421744256E-5	0.0	0.0	8.737388107236946	0.0
26	3.8816274421744256E-5	0.0	0.0	8.949014435384294	0.0
27	7.763254884348851E-5	0.0	0.0	9.153304487665935	0.0
28	7.763254884348851E-5	0.0	0.0	9.383679076358987	0.0
29	7.763254884348851E-5	0.0	0.0	9.618207006415165	0.0
30	7.763254884348851E-5	0.0	0.0	9.899353282051859	0.0
31	7.763254884348851E-5	0.0	0.0	10.172697486529783	0.0
32	7.763254884348851E-5	0.0	0.0	10.416191975977384	0.0
33	7.763254884348851E-5	0.0	0.0	10.666945108741851	0.0
34	7.763254884348851E-5	0.0	0.0	10.924297008158016	0.0
35	7.763254884348851E-5	0.0	0.0	11.215069719851302	0.0
36	7.763254884348851E-5	0.0	0.0	11.499981174106905	0.0
37	7.763254884348851E-5	0.0	0.0	11.791064415995566	0.0
38	7.763254884348851E-5	0.0	0.0	12.05909079087771	0.0
39	7.763254884348851E-5	0.0	0.0	12.364497238027994	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTAATC	90	0.0	45.000004	8
CTATACG	45	3.8562575E-10	45.000004	1
CCGGATA	30	2.1665546E-6	45.000004	20
CCGAATC	30	2.1665546E-6	45.000004	32
TATCGCG	30	2.1665546E-6	45.000004	1
AATACCG	30	2.1665546E-6	45.000004	1
AATCCCG	40	6.8212103E-9	45.0	35
ACCGGAT	35	1.2127202E-7	45.0	10
GTCGAAC	20	7.03492E-4	45.0	37
CATGCGT	20	7.03492E-4	45.0	14
TAGACGT	20	7.03492E-4	45.0	10
ACCGCGA	20	7.03492E-4	45.0	24
CCAATCG	35	1.2127202E-7	45.0	24
CATTACG	40	6.8212103E-9	45.0	28
CGCTCAC	20	7.03492E-4	45.0	22
AGCGCGA	40	6.8212103E-9	45.0	34
GACGATA	20	7.03492E-4	45.0	13
CGTCCCA	20	7.03492E-4	45.0	43
TATCGTG	20	7.03492E-4	45.0	1
CGATACA	35	1.2127202E-7	45.0	10
>>END_MODULE