FastQCFastQC Report
Thu 26 May 2016
SRR1547526_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547526_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2032133
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178730.879519204697724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG302.1663272E-645.00000436
CGACGGT1800.045.00000428
GAATCGT453.8562575E-1045.0000049
CGACGTA253.8918683E-545.029
ATCGTAC253.8918683E-545.041
CGTTAGC207.0345675E-445.026
CGAATGT207.0345675E-445.040
GTATCGC253.8918683E-545.044
AGAATCG253.8918683E-545.015
ACGGTCT1800.043.75000430
TATAGCG1250.043.1999971
CGTTTTT95500.043.1387441
TACGATT556.184564E-1140.90909224
GGCGATA6050.039.793398
TACGGGT2550.039.7058834
GGGCGAT21050.039.6555827
ACTACGG1650.039.5454562
GCGATAT1650.039.5454569
GTCGATC403.4599725E-739.37521
ATAGCGC403.4599725E-739.37538
Produced by FastQC (version 0.11.5)