FastQCFastQC Report
Thu 26 May 2016
SRR1547525_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547525_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3137577
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252900.8060359952919084No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36700.11696924091424687No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA351.2128294E-745.039
TCGGGTA351.2128294E-745.05
CTACGTT207.0351554E-445.028
GCGATAT1550.045.09
CGTAACG207.0351554E-445.012
ACCGTAA207.0351554E-445.020
CGTAGGT302.1667074E-644.99999637
GACGATT302.1667074E-644.9999969
CGACGGT6700.043.32089628
CGGTCTA6800.042.68382331
CGTTTTT112150.042.612571
CGACAGG2400.042.1874962
TAACGCG700.041.7857131
CACGACG6950.041.7625926
CGGGTAC650.041.5384646
CGTAAGG5500.041.3181842
TCACGAC7250.040.3448325
ACGACGG7200.040.312527
CTACCGT451.9301297E-840.027
TTAGCGG7950.039.9056632
Produced by FastQC (version 0.11.5)