##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547525_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3137577
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.534177487915038	31.0	31.0	33.0	30.0	34.0
2	31.952970078503252	31.0	31.0	34.0	30.0	34.0
3	31.962667689111694	33.0	31.0	34.0	30.0	34.0
4	35.718903472329124	37.0	35.0	37.0	33.0	37.0
5	35.66638428315863	37.0	35.0	37.0	33.0	37.0
6	35.72787281395803	37.0	35.0	37.0	33.0	37.0
7	36.10197582401962	37.0	35.0	37.0	35.0	37.0
8	36.11155550923531	37.0	35.0	37.0	35.0	37.0
9	37.94281765833954	39.0	38.0	39.0	35.0	39.0
10	37.396217527091764	39.0	37.0	39.0	35.0	39.0
11	37.05447738812466	39.0	37.0	39.0	33.0	39.0
12	36.56322059984504	39.0	35.0	39.0	33.0	39.0
13	36.33581964681664	39.0	35.0	39.0	32.0	39.0
14	37.299395998887036	40.0	35.0	41.0	32.0	41.0
15	37.49335235438047	40.0	35.0	41.0	32.0	41.0
16	37.60841917186415	40.0	35.0	41.0	33.0	41.0
17	37.57327804226	40.0	35.0	41.0	33.0	41.0
18	37.45760789296964	39.0	36.0	41.0	33.0	41.0
19	37.425583499624075	39.0	36.0	41.0	32.0	41.0
20	37.280634387618214	39.0	35.0	41.0	32.0	41.0
21	37.176137509932026	39.0	35.0	41.0	32.0	41.0
22	37.13262495231193	39.0	35.0	41.0	32.0	41.0
23	37.0418392919122	39.0	35.0	41.0	32.0	41.0
24	36.924765830448145	39.0	35.0	41.0	32.0	41.0
25	36.8336117328754	38.0	35.0	41.0	31.0	41.0
26	36.80386329960986	38.0	35.0	41.0	31.0	41.0
27	36.762896974321265	38.0	35.0	41.0	31.0	41.0
28	36.738681791713795	38.0	35.0	41.0	31.0	41.0
29	36.67788678971066	38.0	35.0	41.0	31.0	41.0
30	36.60816451675927	38.0	35.0	41.0	31.0	41.0
31	36.49718556707931	38.0	35.0	41.0	31.0	41.0
32	36.36500076332788	38.0	35.0	41.0	30.0	41.0
33	36.258511902656096	38.0	35.0	41.0	30.0	41.0
34	36.11833303214551	38.0	35.0	41.0	30.0	41.0
35	35.936098141973886	38.0	35.0	41.0	29.0	41.0
36	35.805173546338466	38.0	35.0	41.0	29.0	41.0
37	35.73637364118873	38.0	35.0	41.0	29.0	41.0
38	35.5615635887183	38.0	35.0	40.0	27.0	41.0
39	35.48368980267257	38.0	35.0	40.0	27.0	41.0
40	35.32715563633976	38.0	34.0	40.0	26.0	41.0
41	35.25156354728505	38.0	34.0	40.0	26.0	41.0
42	35.23086572855423	38.0	34.0	40.0	26.0	41.0
43	35.1338571133075	38.0	34.0	40.0	25.0	41.0
44	34.9930682179274	38.0	34.0	40.0	25.0	41.0
45	34.882592204111646	38.0	34.0	40.0	24.0	41.0
46	34.839303067303206	37.0	34.0	40.0	24.0	41.0
47	34.743887719727674	37.0	34.0	40.0	24.0	41.0
48	34.60218091858781	37.0	34.0	40.0	23.0	41.0
49	34.5220091809699	37.0	34.0	40.0	24.0	41.0
50	34.40283696623222	37.0	34.0	40.0	24.0	41.0
51	34.025047672136814	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	5.0
12	9.0
13	12.0
14	35.0
15	99.0
16	248.0
17	584.0
18	1235.0
19	2379.0
20	4266.0
21	6846.0
22	10437.0
23	15490.0
24	22908.0
25	33133.0
26	45321.0
27	52267.0
28	56541.0
29	62629.0
30	72477.0
31	87954.0
32	108900.0
33	140455.0
34	232239.0
35	352006.0
36	219637.0
37	281190.0
38	439825.0
39	888149.0
40	293.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.8371469449196	23.865167293105475	30.211561341761495	14.086124420213434
2	31.328346682806508	25.91260708502134	29.675160163399973	13.083886068772177
3	28.712665856487344	24.755121547614607	32.59649085902912	13.935721736868928
4	25.40441238573587	27.686173120213464	32.21313771741697	14.696276776633688
5	23.525032214348844	32.18649295300163	30.25579929990563	14.032675532743896
6	22.323149360159128	40.09507973828212	27.14189962509287	10.439871276465883
7	87.56049652327258	3.6316240207013246	7.064623433942817	1.7432560220832827
8	87.92479037167853	3.082091690498751	6.840820161545039	2.152297776277682
9	82.7659687714437	6.0133663651919935	8.263287243627806	2.9573776197365036
10	42.00148713481773	34.39874144921384	14.081853608692313	9.517917807276124
11	33.53734426278622	22.373825407312715	27.295648839853175	16.793181490047893
12	31.841704601990646	21.6444090455788	29.116002571411	17.39788378101956
13	25.24760348510969	28.790401000517278	29.116034443138766	16.845961071234267
14	17.753030443555648	32.372432612809185	31.574810753648435	18.299726189986732
15	16.474081751619163	28.245011994924745	38.759941190287925	16.520965063168173
16	21.358169058480478	26.62723496507018	35.7334975364748	16.28109843997454
17	21.797138365050483	25.806251129454356	29.03119190381622	23.36541860167894
18	22.108939477819987	27.5693313662103	31.451722141002435	18.870007014967282
19	25.620152111007954	28.55601631450001	27.3836466802249	18.440184894267137
20	25.725488171286315	29.792607480230764	27.500584049411376	16.981320299071545
21	23.38935426923387	27.815381104591218	31.702202049543327	17.093062576631585
22	22.542363103758092	24.299674557787746	31.02457724543493	22.133385093019232
23	19.613638167286414	28.267832152007742	31.257145242969337	20.861384437736508
24	18.98337475064357	28.0211768507992	34.48810977387966	18.507338624677576
25	20.180253743573466	29.48386605332714	30.774543541082817	19.561336662016583
26	18.983565981010187	32.80968084607964	27.698539350588046	20.50821382232213
27	18.342816765931165	32.077842233035234	30.97412430037574	18.605216700657863
28	17.09886323108564	29.722234705315596	33.49154458998137	19.687357473617382
29	17.85970511640033	27.911665594183027	34.0592438050126	20.16938548440405
30	20.258817552525404	29.288970438016342	31.599256368847684	18.85295564061057
31	23.971555120400232	28.075677505285128	28.72668941670595	19.226077957608688
32	24.668239217714817	28.187515398028477	28.880821092199486	18.263424292057216
33	23.733154596684003	27.58252626150689	29.115365136855605	19.568954004953504
34	19.08389818002873	28.120393539345805	30.999812912957992	21.79589536766747
35	18.87443718512725	28.22780126192919	31.717372991961636	21.180388560981932
36	24.498490395614194	27.014285227103592	29.472137257507942	19.01508711977427
37	20.24645132215082	30.98569373755608	29.304460097712344	19.463394842580755
38	19.801458259032366	30.22950512449575	28.954189809524994	21.01484680694689
39	19.696887120220477	29.403453684164564	29.17882174684478	21.72083744877018
40	21.931923901787908	27.45500747870092	29.150392165674337	21.462676453836828
41	18.07295884690639	26.819548970431644	29.969368082440685	25.138124100221287
42	20.33750884838842	26.63472482109602	29.192462846330148	23.835303484185406
43	20.72561087743823	26.632015724235615	29.777787126817923	22.864586271508237
44	20.741419254411923	28.635918736018272	28.984085490172827	21.638576519396974
45	18.72938895204803	31.942196159648034	27.026842687844795	22.301572200459145
46	21.112023704916247	30.37382030783627	27.718012976255245	20.79614301099224
47	20.10847861263644	28.48873509717849	29.790982022114516	21.611804268070554
48	20.11475734300704	27.903251458051866	31.350306303239726	20.631684895701362
49	21.123338168274437	26.388451980620715	30.746400805462304	21.741809045642547
50	19.27847507806183	29.481921877933193	29.995024823295175	21.244578220709805
51	18.65917553577171	30.486837454507093	28.48430492701852	22.36968208270267
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2603.0
1	3889.0
2	5175.0
3	21444.0
4	37713.0
5	26176.5
6	14640.0
7	14297.5
8	13955.0
9	15244.0
10	16533.0
11	17565.5
12	18598.0
13	18379.0
14	18160.0
15	17573.0
16	16986.0
17	16073.5
18	15161.0
19	15154.0
20	15147.0
21	17937.5
22	20728.0
23	20133.5
24	19539.0
25	23636.5
26	33217.5
27	38701.0
28	50327.5
29	61954.0
30	68200.0
31	74446.0
32	82654.5
33	90863.0
34	98892.0
35	106921.0
36	119633.5
37	132346.0
38	142305.0
39	152264.0
40	177092.0
41	201920.0
42	225526.5
43	249133.0
44	257473.5
45	265814.0
46	266765.0
47	267716.0
48	258590.0
49	249464.0
50	234902.0
51	220340.0
52	202793.5
53	185247.0
54	165948.5
55	146650.0
56	134709.0
57	122768.0
58	112592.5
59	102417.0
60	89587.5
61	76758.0
62	64675.5
63	52593.0
64	43957.5
65	35322.0
66	28348.5
67	21375.0
68	18713.0
69	16051.0
70	13139.0
71	10227.0
72	7873.5
73	5520.0
74	4630.0
75	2852.0
76	1964.0
77	1533.5
78	1103.0
79	829.5
80	556.0
81	432.5
82	309.0
83	246.5
84	184.0
85	142.5
86	101.0
87	84.0
88	67.0
89	57.5
90	48.0
91	31.5
92	15.0
93	11.5
94	8.0
95	4.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3137577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.575604542774947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.30530465102117	15.524492819926813
2	8.940488888076667	3.50030949784125
3	3.0981983833162516	1.8194731904059083
4	1.5425356435330213	1.2078427100374913
5	0.9199376423802492	0.9004167745624239
6	0.6523205477766578	0.766174144704131
7	0.47280275795781423	0.6478779871560858
8	0.36653502550550593	0.574011576829737
9	0.2930292988509242	0.5162603106377078
>10	2.3730991832909245	10.113542318348042
>50	0.6910256768803618	9.893922063368937
>100	1.3342486398103055	52.098082837309356
>500	0.008509850050146095	1.049584500585583
>1k	0.0018001605875309047	0.577827294114158
>5k	0.0	0.0
>10k+	1.6365096250280952E-4	0.810181974172371
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25290	0.8060359952919084	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3670	0.11696924091424687	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.054628141397007945	0.0
2	0.0	0.0	0.0	0.18574842944093484	0.0
3	0.0	0.0	0.0	0.2733000656238875	0.0
4	0.0	0.0	0.0	0.461120157369843	0.0
5	0.0	0.0	0.0	0.8476923434867096	0.0
6	0.0	0.0	0.0	1.3268518987741178	0.0
7	0.0	0.0	0.0	1.584885406796391	0.0
8	0.0	0.0	0.0	2.0887774228329694	0.0
9	0.0	0.0	0.0	2.2937126323911734	0.0
10	0.0	0.0	0.0	2.637959164030078	0.0
11	0.0	0.0	0.0	3.092577488934933	0.0
12	0.0	0.0	0.0	3.414322580768536	0.0
13	0.0	0.0	0.0	3.559211455208908	0.0
14	0.0	0.0	0.0	3.630667868868238	0.0
15	0.0	0.0	0.0	3.7113989553085074	0.0
16	0.0	0.0	0.0	3.8769407093435477	0.0
17	0.0	0.0	0.0	4.088473366550048	0.0
18	0.0	0.0	0.0	4.329041167754608	0.0
19	0.0	0.0	0.0	4.480750591937664	0.0
20	0.0	0.0	0.0	4.650435670582746	0.0
21	0.0	0.0	0.0	4.869235081720704	0.0
22	0.0	0.0	0.0	5.110440317480655	0.0
23	0.0	0.0	0.0	5.354099676278861	0.0
24	0.0	0.0	0.0	5.53882821043117	0.0
25	0.0	0.0	0.0	5.703636914727511	0.0
26	0.0	0.0	0.0	5.850023760373052	0.0
27	0.0	0.0	0.0	6.024139009178102	0.0
28	0.0	0.0	0.0	6.20115458521018	0.0
29	0.0	0.0	0.0	6.406599742412697	0.0
30	0.0	0.0	0.0	6.638339075025091	0.0
31	0.0	0.0	0.0	6.842573106572365	0.0
32	0.0	0.0	0.0	7.017389533388344	0.0
33	0.0	0.0	0.0	7.199345227224702	0.0
34	0.0	0.0	0.0	7.3973642718569135	0.0
35	0.0	0.0	0.0	7.612849023306838	0.0
36	0.0	0.0	0.0	7.802198957985732	0.0
37	0.0	0.0	0.0	8.01102251833182	0.0
38	0.0	0.0	0.0	8.250666039431064	0.0
39	0.0	0.0	0.0	8.692981877416873	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGA	35	1.2128294E-7	45.0	39
TCGGGTA	35	1.2128294E-7	45.0	5
CTACGTT	20	7.0351554E-4	45.0	28
GCGATAT	155	0.0	45.0	9
CGTAACG	20	7.0351554E-4	45.0	12
ACCGTAA	20	7.0351554E-4	45.0	20
CGTAGGT	30	2.1667074E-6	44.999996	37
GACGATT	30	2.1667074E-6	44.999996	9
CGACGGT	670	0.0	43.320896	28
CGGTCTA	680	0.0	42.683823	31
CGTTTTT	11215	0.0	42.61257	1
CGACAGG	240	0.0	42.187496	2
TAACGCG	70	0.0	41.785713	1
CACGACG	695	0.0	41.76259	26
CGGGTAC	65	0.0	41.538464	6
CGTAAGG	550	0.0	41.318184	2
TCACGAC	725	0.0	40.34483	25
ACGACGG	720	0.0	40.3125	27
CTACCGT	45	1.9301297E-8	40.0	27
TTAGCGG	795	0.0	39.905663	2
>>END_MODULE