FastQCFastQC Report
Thu 26 May 2016
SRR1547524_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547524_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1224072
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54130.44221254958858625No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20800.1699246449555255No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15290.12491095295048005No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAA207.0334657E-445.00000415
CAAGTCG207.0334657E-445.0000041
GCGGTAT207.0334657E-445.00000436
CTATCGG207.0334657E-445.00000421
GCGAAGT207.0334657E-445.00000444
GCAACGC207.0334657E-445.0000049
ACCGCCC207.0334657E-445.00000428
GGCGTAA207.0334657E-445.00000420
GCTATCG207.0334657E-445.00000420
CGATTGA207.0334657E-445.00000410
CGCGAGA207.0334657E-445.00000445
CCTCGGT207.0334657E-445.00000443
TTATCCG207.0334657E-445.0000041
ACCTCGC207.0334657E-445.00000444
CCCGTCG207.0334657E-445.00000412
GTTCGGT207.0334657E-445.00000417
CTACGCT207.0334657E-445.00000429
CGAGGTA406.8157533E-945.00000442
TTACGCT207.0334657E-445.00000428
GCGTGAG207.0334657E-445.00000430