FastQCFastQC Report
Thu 26 May 2016
SRR1547523_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547523_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2829210
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150310.531279049628695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA502.1827873E-1145.00000419
CGGACTA302.166631E-645.00000428
TCCGCGA207.035037E-445.012
ACGTTAC351.2127748E-745.017
ATTACGC207.035037E-445.015
ACCGGGT1350.043.3333324
TATAGCG1200.043.1250041
CGACGGT4450.042.47191228
GTTAGCG700.041.7857131
TCATACG700.041.7857131
CGATCAT603.6379788E-1241.25000410
GCGTTAG3600.040.6251
CGTTTTT71950.040.6219561
TACGGGA11100.040.540544
AATGCGG4900.040.4081652
TAACGGG8600.040.29073
CGGCATA451.9299478E-840.029
CGGGTAT2600.039.8076936
CGTTAGG5250.039.4285742
ATTCGCG403.4606092E-739.3751
Produced by FastQC (version 0.11.5)