FastQCFastQC Report
Thu 26 May 2016
SRR1547522_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547522_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2612537
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123450.4725291928879859No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC46440.17775824801715726No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT43420.16619860312026202No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG36690.14043820240632No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG32440.12417049021698066No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG26600.10181673982033557No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG351.2127202E-745.00000423
ACTATCG750.045.00000424
ACGCCTA351.2127202E-745.00000428
TCCGCGA302.1665674E-645.00000432
ATAGCGC600.045.00000412
TAACGCG302.1665674E-645.00000434
TTATCCG351.2127202E-745.00000418
ACGATGT302.1665674E-645.00000429
ACCTACG351.2127202E-745.00000429
CGGATAT351.2127202E-745.00000420
AACGCAT351.2127202E-745.00000414
CGTAAGA700.045.00000433
ACGTATC302.1665674E-645.00000432
GCGTGAT351.2127202E-745.00000415
ATCGGCC302.1665674E-645.00000426
TTCGCAC600.045.00000441
CAATCGT351.2127202E-745.00000432
TGTCGTA351.2127202E-745.00000426
GATACGT453.8562575E-1045.011
GGTACGT207.034939E-445.07