FastQCFastQC Report
Thu 26 May 2016
SRR1547521_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547521_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3951743
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136360.34506292539772954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAACG302.1668511E-645.00000414
TAACGCG351.2129385E-745.01
CGTAACG502.1827873E-1145.023
CAATCGA351.2129385E-745.019
CGATATA2450.042.244910
CGCAATA750.042.030
CACGACG6050.041.6528926
GCGATAT3500.041.1428579
CGTTTTT82700.040.891781
GTCGTTA501.0822987E-940.539
TCACGAC6400.040.0781325
AGCGTAA451.9303116E-840.036
CCGTCGA451.9303116E-840.036
CGACTAT451.9303116E-840.031
TAGTGCG2250.040.01
GGCGATA7850.039.8407638
ATTAGCG2050.039.5121961
CTCACGA6500.039.4615424
CTATACG1150.039.1304361
TAAGGGA54250.038.7373284
Produced by FastQC (version 0.11.5)