##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547521_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3951743
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5392048521374	31.0	31.0	33.0	30.0	34.0
2	31.971042651306018	31.0	31.0	34.0	30.0	34.0
3	32.05084693007617	33.0	31.0	34.0	30.0	34.0
4	35.77718718044164	37.0	35.0	37.0	35.0	37.0
5	35.71148579247183	37.0	35.0	37.0	33.0	37.0
6	35.763317857461885	37.0	35.0	37.0	33.0	37.0
7	36.086163497980515	37.0	35.0	37.0	35.0	37.0
8	36.07428468905999	37.0	35.0	37.0	35.0	37.0
9	37.89828690782776	39.0	38.0	39.0	35.0	39.0
10	37.37890267661637	39.0	37.0	39.0	35.0	39.0
11	37.067287017399664	39.0	37.0	39.0	33.0	39.0
12	36.654572172330035	39.0	35.0	39.0	33.0	39.0
13	36.48771744518811	39.0	35.0	39.0	32.0	39.0
14	37.60148066308968	40.0	36.0	41.0	33.0	41.0
15	37.71261719195808	40.0	36.0	41.0	33.0	41.0
16	37.76217785417726	40.0	36.0	41.0	33.0	41.0
17	37.68979865340433	40.0	35.0	41.0	33.0	41.0
18	37.563940266358415	39.0	36.0	41.0	32.0	41.0
19	37.55538783772123	39.0	36.0	41.0	32.0	41.0
20	37.4388564742191	39.0	35.0	41.0	32.0	41.0
21	37.32443329437162	39.0	35.0	41.0	32.0	41.0
22	37.26497826402172	39.0	35.0	41.0	32.0	41.0
23	37.1799790624036	39.0	35.0	41.0	32.0	41.0
24	37.08754111793201	39.0	35.0	41.0	32.0	41.0
25	36.98403210937553	39.0	35.0	41.0	31.0	41.0
26	36.9556375503164	39.0	35.0	41.0	31.0	41.0
27	36.92361446581926	39.0	35.0	41.0	31.0	41.0
28	36.89767401372002	39.0	35.0	41.0	31.0	41.0
29	36.858911371513784	39.0	35.0	41.0	31.0	41.0
30	36.80988490395251	39.0	35.0	41.0	31.0	41.0
31	36.77812676583472	39.0	35.0	41.0	31.0	41.0
32	36.67178356487251	39.0	35.0	41.0	31.0	41.0
33	36.59549419079125	39.0	35.0	41.0	30.0	41.0
34	36.4693316341675	39.0	35.0	41.0	30.0	41.0
35	36.31882361783142	39.0	35.0	41.0	30.0	41.0
36	36.22445234925449	39.0	35.0	41.0	30.0	41.0
37	36.20485821066805	39.0	35.0	41.0	30.0	41.0
38	36.08082408193043	39.0	35.0	41.0	29.0	41.0
39	35.99238538538564	38.0	35.0	41.0	29.0	41.0
40	35.843557387208634	38.0	35.0	41.0	28.0	41.0
41	35.78445739006813	38.0	35.0	41.0	28.0	41.0
42	35.76469547741338	38.0	35.0	41.0	28.0	41.0
43	35.67375358164739	38.0	35.0	40.0	28.0	41.0
44	35.53270873131173	38.0	34.0	40.0	27.0	41.0
45	35.42118579067515	38.0	34.0	40.0	27.0	41.0
46	35.35069234006361	38.0	34.0	40.0	27.0	41.0
47	35.29932386797421	38.0	34.0	40.0	27.0	41.0
48	35.18233625010534	38.0	34.0	40.0	26.0	41.0
49	35.065553352027194	38.0	34.0	40.0	26.0	41.0
50	34.963238500074524	37.0	34.0	40.0	26.0	41.0
51	34.58835658088089	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	23.0
12	32.0
13	35.0
14	53.0
15	144.0
16	400.0
17	785.0
18	1685.0
19	3172.0
20	5224.0
21	8045.0
22	11931.0
23	17249.0
24	24877.0
25	35141.0
26	47191.0
27	56517.0
28	63383.0
29	71779.0
30	84780.0
31	103536.0
32	129910.0
33	166952.0
34	277211.0
35	401317.0
36	272468.0
37	366607.0
38	595795.0
39	1205137.0
40	355.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.44239668419733	23.443781642682733	29.408972192776705	12.704849480343231
2	31.197904317158276	25.02958820955715	29.583021972835784	14.18948550044879
3	29.69659211137971	25.609408304133137	31.01193574582153	13.682063838665623
4	26.593784059337867	27.38308133904457	30.613503965212313	15.409630636405252
5	24.445086636453837	31.702162817774333	28.72142748149361	15.131323064278218
6	23.066580999827167	40.55638233559217	25.932278490782423	10.444758173798245
7	87.9630330211251	3.7785351932046183	5.980095365513395	2.2783364201568777
8	87.90946172359892	3.695382012443623	6.023797600198192	2.3713586637592576
9	82.71365319050354	6.313416636658811	7.561726559647224	3.4112036131904326
10	40.30343066338069	35.622002746636106	13.488706122842503	10.585860467140702
11	32.03621794231052	22.824535907319884	27.442067968488843	17.697178181880755
12	30.69435436464365	22.3101046803904	28.616157477852177	18.37938347711377
13	25.026678101283405	27.675635789068266	28.646118940427044	18.651567169221277
14	18.61411534100269	30.78725008179935	30.257762207714418	20.34087236948354
15	18.667408280346166	28.300600519821256	35.774112840840104	17.257878358992475
16	22.508270401187527	26.707328892592457	32.75000423863597	18.034396467584052
17	22.879802659231636	25.922738396702417	27.33892360915171	23.858535334914237
18	23.589489498684504	27.282619340377146	30.557452749331116	18.57043841160723
19	26.37995436444121	27.163709785783137	27.421444157679282	19.034891692096373
20	27.333558887812288	26.91731218350991	27.8562396390656	17.892889289612203
21	26.60590529293023	25.878681887966902	29.82623110865256	17.6891817104503
22	24.54484008701983	23.546824780862522	30.16901655800997	21.739318574107678
23	21.482014392130257	26.50210299607034	30.216792944278005	21.799089667521397
24	21.049445776205587	26.209320798442608	33.50794826485427	19.23328516049753
25	20.910013631959366	28.314796787139247	30.338561996567087	20.436627584334303
26	20.29906803149901	31.307071335357588	27.658934298105926	20.734926335037475
27	19.266055510188796	30.708803684855013	30.669302128200137	19.355838676756054
28	18.22304739959051	28.867590832703442	33.32767338361832	19.58168838408773
29	19.578499917631284	26.470901574317967	33.25254197957711	20.69805652847364
30	20.643068134744595	27.145743030353948	31.6729099033009	20.538278931600562
31	24.14230884953804	26.61683717792377	29.109863672814758	20.13099029972344
32	24.900657760385734	27.038372687697553	28.727956246142526	19.333013305774184
33	23.874629498932496	27.20991724411228	28.38228599380071	20.53316726315451
34	20.85859328402682	27.219077758852233	30.08614173543168	21.836187221689265
35	21.054506834072964	27.485947340198997	29.754212255199796	21.705333570528246
36	23.739499253873543	27.381537716395023	28.880091645635865	19.998871384095576
37	21.346631094177937	29.67310880287509	28.954337364550277	20.025922738396705
38	21.169316931794402	30.071161004144248	27.63297107124628	21.12655099281507
39	21.173340472798966	29.323693367711414	28.41695424019224	21.086011919297384
40	23.151404329684393	27.186054356267604	27.943618803145853	21.71892251090215
41	20.423013338671062	26.91245356795723	29.195117192590715	23.469415900780994
42	21.64905460704302	26.786534448216905	27.560724470189484	24.003686474550598
43	21.620282493066984	26.92373972700148	28.16061165920962	23.29536612072192
44	20.746718599868462	28.47204385507863	28.724843695554085	22.056393849498814
45	20.918111324547166	29.80229230493987	26.84506558245311	22.43453078805985
46	21.45043339103783	29.225863119135028	28.052203799690417	21.271499690136732
47	20.89485576364657	27.760459118925496	29.250409249791804	22.094275867636128
48	21.94871984337038	26.781422779770853	30.38072060860233	20.88913676825644
49	21.8737402710652	26.588723001470488	29.894302336968774	21.643234390495536
50	20.39396286651232	28.79534423164664	29.528413158446792	21.282279743394245
51	19.916477362014685	28.87662482099671	29.110546915626852	22.096350901361753
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2231.0
1	4480.0
2	6729.0
3	16900.0
4	27071.0
5	20235.5
6	13400.0
7	13082.5
8	12765.0
9	13075.0
10	13385.0
11	13354.5
12	13324.0
13	13031.0
14	12738.0
15	12328.0
16	11918.0
17	12078.5
18	12239.0
19	14161.0
20	16083.0
21	15872.0
22	15661.0
23	18926.5
24	22192.0
25	28903.5
26	44220.0
27	52825.0
28	62291.5
29	71758.0
30	81035.0
31	90312.0
32	99962.5
33	109613.0
34	128461.0
35	147309.0
36	154644.5
37	161980.0
38	176594.0
39	191208.0
40	216028.0
41	240848.0
42	272684.0
43	304520.0
44	305355.0
45	306190.0
46	309089.5
47	311989.0
48	314242.5
49	316496.0
50	299611.5
51	282727.0
52	256848.0
53	230969.0
54	218766.5
55	206564.0
56	190734.5
57	174905.0
58	166873.0
59	158841.0
60	144289.0
61	129737.0
62	109670.5
63	89604.0
64	77764.0
65	65924.0
66	51547.5
67	37171.0
68	30578.0
69	23985.0
70	18311.5
71	12638.0
72	10254.5
73	7871.0
74	6352.5
75	3936.0
76	3038.0
77	2120.5
78	1203.0
79	886.5
80	570.0
81	425.5
82	281.0
83	268.0
84	255.0
85	160.5
86	66.0
87	60.5
88	55.0
89	52.5
90	50.0
91	44.5
92	39.0
93	24.5
94	10.0
95	7.0
96	4.0
97	3.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3951743.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.192539803396027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.71287143505116	13.231389231618481
2	8.38523782821456	2.7155659458861265
3	2.5540828580940764	1.2407126502257948
4	1.1541232428412578	0.7475274619092628
5	0.6273473635891809	0.5079173577736686
6	0.41861166807589895	0.4067031658491
7	0.2974356585819213	0.3371367118375951
8	0.21901916640718147	0.28371812558039233
9	0.17059625356971103	0.2486147963614009
>10	1.7548888848057664	6.790596158719518
>50	0.8500308188275738	10.148917794547083
>100	1.843627787918	61.33039759295742
>500	0.009764624798327886	1.0025197468258733
>1k	0.0022049152770417806	0.6605343024226539
>5k	0.0	0.0
>10k+	1.5749394836012717E-4	0.34774895748563023
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13636	0.34506292539772954	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2652644668441243E-4	0.0	0.0	0.02312903445391059	0.0
2	1.2652644668441243E-4	0.0	0.0	0.08505107746126203	0.0
3	1.2652644668441243E-4	0.0	0.0	0.12156660997438345	0.0
4	1.2652644668441243E-4	0.0	0.0	0.19826694195447428	0.0
5	1.2652644668441243E-4	0.0	0.0	0.3597399932131214	0.0
6	1.2652644668441243E-4	0.0	0.0	0.5159748495790338	0.0
7	1.2652644668441243E-4	0.0	0.0	0.6063400378010412	0.0
8	1.2652644668441243E-4	0.0	0.0	0.8024560301618805	0.0
9	1.2652644668441243E-4	0.0	0.0	0.8659723063974555	0.0
10	1.2652644668441243E-4	0.0	0.0	1.0034053327860644	0.0
11	1.2652644668441243E-4	0.0	0.0	1.1904620315642995	0.0
12	1.2652644668441243E-4	0.0	0.0	1.3395354910478743	0.0
13	1.2652644668441243E-4	0.0	0.0	1.400318796035066	0.0
14	1.2652644668441243E-4	0.0	0.0	1.4248143161131683	0.0
15	1.2652644668441243E-4	0.0	0.0	1.467124759884436	0.0
16	1.2652644668441243E-4	0.0	0.0	1.5575658639744538	0.0
17	1.2652644668441243E-4	0.0	0.0	1.6701997068129177	0.0
18	1.2652644668441243E-4	0.0	0.0	1.8118587165207858	0.0
19	1.2652644668441243E-4	0.0	0.0	1.893873159261622	0.0
20	1.2652644668441243E-4	0.0	0.0	1.9869460134426757	0.0
21	1.2652644668441243E-4	0.0	0.0	2.1043119453871366	0.0
22	1.2652644668441243E-4	0.0	0.0	2.2363296398576527	0.0
23	1.2652644668441243E-4	0.0	0.0	2.368322029038832	0.0
24	1.2652644668441243E-4	0.0	0.0	2.467468152660737	0.0
25	1.2652644668441243E-4	0.0	0.0	2.558794941877546	0.0
26	1.2652644668441243E-4	0.0	0.0	2.6470344857952552	0.0
27	1.2652644668441243E-4	0.0	0.0	2.7360331883930713	0.0
28	1.2652644668441243E-4	0.0	0.0	2.839810179963626	0.0
29	1.2652644668441243E-4	0.0	0.0	2.9502171573404445	0.0
30	1.2652644668441243E-4	0.0	0.0	3.077376236258279	0.0
31	1.2652644668441243E-4	0.0	0.0	3.1928948820811476	0.0
32	1.2652644668441243E-4	0.0	0.0	3.304541818635473	0.0
33	1.2652644668441243E-4	0.0	0.0	3.416947913869905	0.0
34	1.518317360212949E-4	0.0	0.0	3.541399326828693	0.0
35	1.518317360212949E-4	0.0	0.0	3.675567970892844	0.0
36	1.771370253581774E-4	0.0	0.0	3.8014111747651604	0.0
37	1.771370253581774E-4	0.0	0.0	3.9305693715406087	0.0
38	1.771370253581774E-4	0.0	0.0	4.0730887610859305	0.0
39	1.771370253581774E-4	0.0	0.0	4.259411606473397	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTAACG	30	2.1668511E-6	45.000004	14
TAACGCG	35	1.2129385E-7	45.0	1
CGTAACG	50	2.1827873E-11	45.0	23
CAATCGA	35	1.2129385E-7	45.0	19
CGATATA	245	0.0	42.2449	10
CGCAATA	75	0.0	42.0	30
CACGACG	605	0.0	41.65289	26
GCGATAT	350	0.0	41.142857	9
CGTTTTT	8270	0.0	40.89178	1
GTCGTTA	50	1.0822987E-9	40.5	39
TCACGAC	640	0.0	40.07813	25
AGCGTAA	45	1.9303116E-8	40.0	36
CCGTCGA	45	1.9303116E-8	40.0	36
CGACTAT	45	1.9303116E-8	40.0	31
TAGTGCG	225	0.0	40.0	1
GGCGATA	785	0.0	39.840763	8
ATTAGCG	205	0.0	39.512196	1
CTCACGA	650	0.0	39.46154	24
CTATACG	115	0.0	39.130436	1
TAAGGGA	5425	0.0	38.737328	4
>>END_MODULE