FastQCFastQC Report
Thu 26 May 2016
SRR1547520_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547520_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5273670
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT992771.8825030766050965No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73570.1395043679259415No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA207.0355926E-445.032
CGTTTTT423250.043.9049031
GCGCGAC2100.041.7857139
CGACGGT5200.041.1057728
TACGGGA33500.040.7014924
GACGCGT501.0822987E-940.4999969
GCGCGAG3850.040.3246731
TAATCGC451.9304935E-840.037
CGTATGG6000.039.752
ACGGGAT35800.039.720675
TCGACGG2050.039.5121962
CGGTTTT30450.039.2364541
TATCGCG1100.038.8636361
TAGGGAC77300.038.800135
CTATGCG2850.038.684211
AAGTCGC356.252558E-638.57142632
TAGGGAT114050.038.3910565
TAACGGG15600.038.2211533
AGGGATT132150.038.172536
CGAGGGA42150.038.0604974
Produced by FastQC (version 0.11.5)