FastQCFastQC Report
Thu 26 May 2016
SRR1547519_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547519_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2440771
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162280.6648718785990165No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT60680.24860996791587578No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32970.13508026766951917No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31860.13053252435398485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGT302.1665091E-645.00000431
CGCCGTA302.1665091E-645.00000430
TCGCCGG253.892099E-545.020
ACGCCTA207.0348463E-445.018
CTATACG207.0348463E-445.01
GGTCCGC253.892099E-545.043
ATAGCCG253.892099E-545.015
CGCGCCA207.0348463E-445.017
TGCGGTA453.8562575E-1045.024
CCGTCTA502.1827873E-1145.027
GTTCGCT253.892099E-545.043
CGCTATA253.892099E-545.025
CACTACG207.0348463E-445.032
TCGCTGC207.0348463E-445.016
TCGTCGC351.2126839E-745.039
GTCGTCG253.892099E-545.044
TCCGTCG253.892099E-545.041
ACGTCGT253.892099E-545.044
ATACGTC406.8212103E-945.028
CGCGTAT207.0348463E-445.031