FastQCFastQC Report
Thu 26 May 2016
SRR1547518_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547518_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2091806
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273091.3055225962637071No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25510.12195203570503192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGC351.2125747E-745.00000413
CGCGTCA351.2125747E-745.00000439
CCGCTTA207.0346147E-445.040
CCCGTCG207.0346147E-445.020
AGCGCGA551.8189894E-1245.036
GCGCTAA253.891907E-545.034
CCCGACG207.0346147E-445.010
CGTATTA302.16636E-644.99999638
TACGCAT302.16636E-644.99999645
CGACGGT3900.043.84615328
CGTTTTT107050.043.5287251
TCACGAC4050.042.22222525
CTCGTCC3100.042.0967737
GTAAGCG700.041.7857171
AAGCGAC650.041.5384615
TATCGTG1300.041.538461
CGGGTCC603.6379788E-1241.2499966
GCGCGAC603.6379788E-1241.2499969
CTATCGA556.184564E-1140.90909241
CGTAAGG2200.040.9090922