##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547518_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2091806
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.662928588980048	31.0	31.0	33.0	30.0	34.0
2	32.0861662123543	33.0	31.0	34.0	30.0	34.0
3	32.00641072833714	33.0	31.0	34.0	30.0	34.0
4	35.79717813219773	37.0	35.0	37.0	35.0	37.0
5	35.77761322034644	37.0	35.0	37.0	35.0	37.0
6	35.849613205048655	37.0	35.0	37.0	35.0	37.0
7	36.21337686190785	37.0	35.0	37.0	35.0	37.0
8	36.24985443200756	37.0	35.0	37.0	35.0	37.0
9	38.122361251473606	39.0	39.0	39.0	37.0	39.0
10	37.58510253818949	39.0	37.0	39.0	35.0	39.0
11	37.19594360088842	39.0	37.0	39.0	34.0	39.0
12	36.88139913548388	39.0	35.0	39.0	33.0	39.0
13	36.719160381029596	39.0	35.0	39.0	33.0	39.0
14	37.820727161122974	40.0	36.0	41.0	33.0	41.0
15	37.97380349802993	40.0	36.0	41.0	33.0	41.0
16	38.05957866073623	40.0	36.0	41.0	33.0	41.0
17	37.99897457029954	40.0	36.0	41.0	33.0	41.0
18	37.84585807670501	39.0	36.0	41.0	33.0	41.0
19	37.801826268784005	39.0	36.0	41.0	33.0	41.0
20	37.64079603940327	39.0	35.0	41.0	33.0	41.0
21	37.53799635339033	39.0	35.0	41.0	33.0	41.0
22	37.486747815045945	39.0	35.0	41.0	33.0	41.0
23	37.420425221076904	39.0	35.0	41.0	33.0	41.0
24	37.33151831479592	39.0	35.0	41.0	33.0	41.0
25	37.24762717001481	39.0	35.0	41.0	33.0	41.0
26	37.220998027541754	39.0	35.0	41.0	33.0	41.0
27	37.18415522280747	39.0	35.0	41.0	32.0	41.0
28	37.168136528913294	39.0	35.0	41.0	33.0	41.0
29	37.12654137142737	39.0	35.0	41.0	32.0	41.0
30	37.05002471548509	39.0	35.0	41.0	32.0	41.0
31	36.950421788636234	39.0	35.0	41.0	32.0	41.0
32	36.782554883196624	39.0	35.0	41.0	31.0	41.0
33	36.62723742067859	39.0	35.0	41.0	31.0	41.0
34	36.44751951184766	39.0	35.0	41.0	30.0	41.0
35	36.23005861920274	39.0	35.0	41.0	30.0	41.0
36	36.08674465987764	39.0	35.0	41.0	30.0	41.0
37	36.03448837989756	39.0	35.0	41.0	29.0	41.0
38	35.880456409437585	39.0	35.0	41.0	29.0	41.0
39	35.792083969545935	39.0	35.0	41.0	28.0	41.0
40	35.65527826194207	39.0	35.0	41.0	27.0	41.0
41	35.59520433539248	38.0	35.0	41.0	27.0	41.0
42	35.57168829231774	38.0	35.0	41.0	27.0	41.0
43	35.49931972659032	38.0	35.0	41.0	26.0	41.0
44	35.36473363208634	38.0	35.0	41.0	26.0	41.0
45	35.27343357844848	38.0	34.0	40.0	26.0	41.0
46	35.239595354444916	38.0	34.0	40.0	26.0	41.0
47	35.16562577982853	38.0	34.0	40.0	25.0	41.0
48	35.035076388536986	38.0	34.0	40.0	24.0	41.0
49	34.97235068644033	38.0	34.0	40.0	24.0	41.0
50	34.88072698902288	38.0	34.0	40.0	24.0	41.0
51	34.542102852750205	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	1.0
12	1.0
13	10.0
14	15.0
15	31.0
16	80.0
17	250.0
18	528.0
19	1099.0
20	2087.0
21	3375.0
22	5543.0
23	8477.0
24	13146.0
25	21145.0
26	29860.0
27	34129.0
28	35015.0
29	36498.0
30	41450.0
31	49947.0
32	62297.0
33	81919.0
34	135595.0
35	219761.0
36	141756.0
37	187755.0
38	299026.0
39	680775.0
40	232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.008375537693265	24.04768893482474	31.033566210250857	14.91036931723114
2	31.196248600491632	25.50585474943661	30.643138034789075	12.65475861528268
3	27.00604166925614	24.80669813548675	35.40103623376164	12.786223961495475
4	24.721317368819097	27.489786337738774	34.15703941952552	13.631856873916606
5	22.924401211202188	31.345880067272013	32.394208640763054	13.335510080762747
6	21.34973319705556	40.00217993446811	28.559484005686954	10.08860286278938
7	88.499220290983	2.824210275713905	7.524885194898571	1.1516842384045176
8	90.12747836080402	1.818189640913163	7.123461735935359	0.9308702623474644
9	85.50625631631232	5.132885171951893	7.90876400583993	1.4520945058958623
10	40.548406496587155	40.19411933993879	12.435522223380179	6.821951940093871
11	26.771172852549423	24.47067271056685	32.69184618458882	16.066308252294906
12	28.647446273698424	22.482629842346757	32.079026448915435	16.790897435039387
13	23.150378189946867	27.856407334140933	31.664265233009182	17.328949242903022
14	15.538821477708737	32.96730193908996	32.81695338860296	18.676923194598352
15	14.979257158646645	30.24988933008128	39.607353645605755	15.163499865666319
16	20.918048805673184	27.431750363083385	36.912648687306564	14.737552143936867
17	20.134324119923168	26.700229371174956	30.975243402112817	22.19020310678906
18	20.523843989356568	28.936239785142597	33.90567767756666	16.634238547934178
19	23.301204796238277	28.807069106790973	30.619139633407688	17.272586463563062
20	25.12360132823025	29.035053919914176	30.26346611492653	15.577878636929047
21	23.102190164862325	27.534341138709806	33.80566840328405	15.55780029314382
22	22.006438455573797	23.68168941096832	34.06491806601568	20.2469540674422
23	18.183521798866625	28.23765683815803	33.97279671250584	19.606024650469497
24	17.886696949908355	28.875239864499864	36.51504967477864	16.723013510813146
25	18.708618294430746	30.36428808407663	32.75757885769522	18.169514763797405
26	18.186724772756175	33.442298186351884	29.959327012160784	18.411650028731156
27	16.886938846145387	32.68663537632075	33.99464386276739	16.431781914766475
28	15.392440790398346	30.614215658622264	36.6556936924361	17.337649858543287
29	16.667128787277598	28.633869488853176	36.02982303330232	18.669178690566905
30	18.6987703448599	30.116846399713932	34.100915668087765	17.083467587338404
31	22.564951051866185	29.590793792540993	30.54069067590398	17.303564479688845
32	22.786051861405884	29.682389284665977	31.014874228298417	16.51668462562972
33	22.406714580606423	29.661402634852372	30.36137194366973	17.570510840871474
34	18.84997939579483	30.03591155202729	31.42829688795232	19.685812164225556
35	18.643220260387437	29.590172319995254	31.975049311456228	19.79155810816108
36	22.55146987818182	29.545139463219822	30.97361801237782	16.92977264622054
37	19.330425479227042	30.967355481340046	31.679993268974272	18.022225770458636
38	19.091875632826373	31.85854711192147	29.446325328448243	19.603251926803917
39	18.62610586258955	30.929780295113407	30.760596345932655	19.683517496364384
40	21.27969802170947	29.318971262153376	29.841677478695445	19.55965323744171
41	18.464618611859798	28.833983648579263	31.163788611372183	21.537609128188752
42	19.64187883580026	28.927730391824095	30.363140750146044	21.067250022229594
43	19.666164070664298	29.613023387446063	29.52855092680679	21.192261615082852
44	19.244423240013653	30.847554696754862	30.30959850005211	19.598423563179377
45	18.687488227875818	33.34563530270015	28.104470491049362	19.862405978374667
46	20.736961266962613	32.35094459046393	28.60886717028252	18.30322697229093
47	19.561708877400676	30.102409114420748	30.64763175935053	19.688250248828044
48	19.71425648458796	30.338568681799366	31.469505298292482	18.477669535320196
49	20.12079514065836	29.164798265231095	30.75113084100533	19.96327575310521
50	18.491724375969856	30.90348722587085	31.189986069453862	19.414802328705434
51	17.290322333906683	32.47638643354116	29.746353151296056	20.4869380812561
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1235.0
1	2351.0
2	3467.0
3	21438.0
4	39409.0
5	26774.0
6	14139.0
7	13385.5
8	12632.0
9	13489.5
10	14347.0
11	15282.0
12	16217.0
13	16165.5
14	16114.0
15	15954.5
16	15795.0
17	15138.5
18	14482.0
19	14177.5
20	13873.0
21	14321.0
22	14769.0
23	16800.5
24	18832.0
25	21955.5
26	31070.5
27	37062.0
28	42910.0
29	48758.0
30	57279.5
31	65801.0
32	72028.0
33	78255.0
34	90101.0
35	101947.0
36	104989.0
37	108031.0
38	111659.5
39	115288.0
40	128931.5
41	142575.0
42	156512.0
43	170449.0
44	172970.5
45	175492.0
46	168854.0
47	162216.0
48	155358.0
49	148500.0
50	138939.5
51	129379.0
52	113372.0
53	97365.0
54	90034.0
55	82703.0
56	74322.0
57	65941.0
58	58660.5
59	51380.0
60	44741.5
61	38103.0
62	30663.0
63	23223.0
64	18253.0
65	13283.0
66	10526.0
67	7769.0
68	5775.5
69	3782.0
70	2859.0
71	1936.0
72	1387.0
73	838.0
74	752.5
75	519.0
76	371.0
77	252.5
78	134.0
79	105.5
80	77.0
81	64.0
82	51.0
83	35.5
84	20.0
85	14.5
86	9.0
87	8.5
88	8.0
89	4.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2091806.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.12532219655516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.12204615704871	15.923566717613143
2	7.681955206454044	3.0920364725878406
3	2.8615617095202013	1.7276955416825772
4	1.5052633548357124	1.2117564002694499
5	1.0241595105260766	1.030577006500176
6	0.7003421617441278	0.8456766991759512
7	0.5162707544490467	0.7273080691761976
8	0.38656823451706523	0.6223848216487547
9	0.3046196664886454	0.551751204194205
>10	3.4509390315561337	16.50254092703994
>50	1.294151721854791	18.886077507344783
>100	1.1463952872618302	36.55783187444971
>500	0.004295402807750868	0.5665118483798957
>1k	0.0011931674465974634	0.4427494684820497
>5k	0.0	0.0
>10k+	2.3863348931949267E-4	1.3115354414552873
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27309	1.3055225962637071	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2551	0.12195203570503192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024572068346682244	0.0
2	0.0	0.0	0.0	0.08136509791060931	0.0
3	0.0	0.0	0.0	0.11894028413724791	0.0
4	0.0	0.0	0.0	0.17511184115544176	0.0
5	0.0	0.0	0.0	0.32211400101156606	0.0
6	0.0	0.0	0.0	0.47112399524621307	0.0
7	0.0	0.0	0.0	0.5671654063522144	0.0
8	0.0	0.0	0.0	0.7618297299080317	0.0
9	0.0	0.0	0.0	0.8481188026040656	0.0
10	4.780558044101604E-5	0.0	0.0	1.007693830116177	0.0
11	4.780558044101604E-5	0.0	0.0	1.2760265531316002	0.0
12	4.780558044101604E-5	0.0	0.0	1.4558233411702615	0.0
13	4.780558044101604E-5	0.0	0.0	1.5290614904058981	0.0
14	4.780558044101604E-5	0.0	0.0	1.5655849538628344	0.0
15	4.780558044101604E-5	0.0	0.0	1.618171092347952	0.0
16	4.780558044101604E-5	0.0	0.0	1.7151208094823325	0.0
17	4.780558044101604E-5	0.0	0.0	1.8586809675467038	0.0
18	4.780558044101604E-5	0.0	0.0	2.0072607115573815	0.0
19	4.780558044101604E-5	0.0	0.0	2.11209834946453	0.0
20	4.780558044101604E-5	0.0	0.0	2.219947738939462	0.0
21	4.780558044101604E-5	0.0	0.0	2.3469671661712415	0.0
22	4.780558044101604E-5	0.0	0.0	2.500853329610872	0.0
23	4.780558044101604E-5	0.0	0.0	2.6572731888138765	0.0
24	4.780558044101604E-5	0.0	0.0	2.774874916698776	0.0
25	4.780558044101604E-5	0.0	0.0	2.8751232188835867	0.0
26	4.780558044101604E-5	0.0	0.0	2.9824467469736677	0.0
27	4.780558044101604E-5	0.0	0.0	3.1085578681770683	0.0
28	4.780558044101604E-5	0.0	0.0	3.222478566368009	0.0
29	4.780558044101604E-5	0.0	0.0	3.3604932771012224	0.0
30	4.780558044101604E-5	0.0	0.0	3.5095988824967517	0.0
31	4.780558044101604E-5	0.0	0.0	3.640920811968223	0.0
32	4.780558044101604E-5	0.0	0.0	3.7659802104019207	0.0
33	4.780558044101604E-5	0.0	0.0	3.895915778040602	0.0
34	4.780558044101604E-5	0.0	0.0	4.034886600382636	0.0
35	4.780558044101604E-5	0.0	0.0	4.189203014046235	0.0
36	4.780558044101604E-5	0.0	0.0	4.336300785063242	0.0
37	4.780558044101604E-5	0.0	0.0	4.493867978196831	0.0
38	4.780558044101604E-5	0.0	0.0	4.665059761756109	0.0
39	4.780558044101604E-5	0.0	0.0	4.933918346156384	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGC	35	1.2125747E-7	45.000004	13
CGCGTCA	35	1.2125747E-7	45.000004	39
CCGCTTA	20	7.0346147E-4	45.0	40
CCCGTCG	20	7.0346147E-4	45.0	20
AGCGCGA	55	1.8189894E-12	45.0	36
GCGCTAA	25	3.891907E-5	45.0	34
CCCGACG	20	7.0346147E-4	45.0	10
CGTATTA	30	2.16636E-6	44.999996	38
TACGCAT	30	2.16636E-6	44.999996	45
CGACGGT	390	0.0	43.846153	28
CGTTTTT	10705	0.0	43.528725	1
TCACGAC	405	0.0	42.222225	25
CTCGTCC	310	0.0	42.09677	37
GTAAGCG	70	0.0	41.785717	1
AAGCGAC	65	0.0	41.53846	15
TATCGTG	130	0.0	41.53846	1
CGGGTCC	60	3.6379788E-12	41.249996	6
GCGCGAC	60	3.6379788E-12	41.249996	9
CTATCGA	55	6.184564E-11	40.909092	41
CGTAAGG	220	0.0	40.909092	2
>>END_MODULE